Journal of Applied Biomedicine, 2006 (vol. 4), issue 3
Original Research Article
Improvement of the interleukin 2 and tumour necrosis factor α release by blood leukocytes as well as of plasma cortisol and antioxidant levels after acupuncture treatment in women suffering anxiety
Lorena Arranz, León Siboni, Mónica De la Fuente
J Appl Biomed 4:115-122, 2006 | DOI: 10.32725/jab.2006.012 
In the context of psychoneuroimmunology, which studies the complex interactions between the nervous system and the immune system, anxiety-causing situations affect the immune function leading to its impairment, whereas acupuncture provides the possibility of improving it via its action on the nervous system. Therefore, the aim of the present work was to study, in anxious adult women, the effect of an acupuncture treatment specifically designed to relieve their symptoms, on several parameters known to be altered in disturbed emotional situations. These include the release by blood leukocytes of the cytokines interleukin-2 (IL-2) and tumour necrosis...
Comparison of the neuroprotective effects of the newly developed oximes (K027, K048) with trimedoxime in tabun-poisoned rats
Jiří Kassa, Gabriela Kunešová
J Appl Biomed 4:123-134, 2006 | DOI: 10.32725/jab.2006.013
Tabun (O-ethyl-N,N-dimethyl phosphoramidocyanidate) is one of the highly toxic organophosphorus compounds misused as chemical warfare agents for military as well as terroristic purposes. It differs from other highly toxic organophosphates in its chemical structure and by the fact that the commonly used antidotes (atropine in combination with an oxime) are not able to sufficiently eliminate its acute toxic effects.The neuroprotective effects of the newly developed oximes (K027, K048) or trimedoxime in combination with atropine (atropine, K027/atropine, K048/atropine and trimedoxime/atropine mixtures) on rats poisoned with tabun at a lethal dose...
Parthenolide has apoptotic and cytotoxic selective effect on B-chronic lymphocytic leukemia cells
Gustavo Horacio Marin, Eduardo Mansilla
J Appl Biomed 4:135-139, 2006 | DOI: 10.32725/jab.2006.014
B-chronic lymphocytic leukemia (B-CLL) is the most common form of leukemia in the western world. It results from a relentless accumulation of small mature monoclonal lymphocytes. Following a recent demonstration of a significant increase in the proliferative pool of CLL cells in vivo, the gradual accumulation of malignant B-CLL cells seems to be primarily the consequence of their selective survival advantages relative to their normal B-cell counterparts. As the disease is mainly caused by defective apoptosis it is thus a good candidate for treatment by proapoptotic agents. Even though a large amount of research has been done during the last past years,...
Effects of α-ketoglutarate on antioxidants and lipid peroxidation products in rats treated with sodium valproate
Murugesan Vidya, Perumal Subramanian
J Appl Biomed 4:141-146, 2006 | DOI: 10.32725/jab.2006.015
Oxidative stress may cause free radical reactions to produce deleterious modifications in membranes, proteins, enzymes and DNA. Valproic acid is a major anti-epileptic drug with a broad spectrum of anti-epileptic activity. Chronic treatment with valproic acid can lead to elevated serum ammonia levels and specific oxidative metabolites of valproic acid have been associated with the drug's toxicity. The influence of sodium valproate treatment on lipid peroxidation and lipid profiles and the detoxifying effects of α-ketoglutarate on sodium valproate induced toxicity were studied in rats. The levels of thiobarbituric acid reactive substances, hydroperoxides...
Reactivation of acetycholinesterase inhibited by the pesticide chlorpyrifos
Veronika Račáková, Daniel Jun, Veronika Opletalová, Kamil Kuča
J Appl Biomed 4:147-151, 2006 | DOI: 10.32725/jab.2006.016
Organophosphorus pesticides such as parathion or chlorpyrifos are substances used worldwide for agricultural purposes. These compounds are able to inhibit an enzyme acetylcholinesterase (AChE; EC 3.1.1.7) by phosphorylation in its active site. AChE reactivators and anticholinergics are generally used as antidotes in the case of intoxication by these agents. In this work, the reactivation potency of nine structurally different AChE reactivators was tested in vitro. Chlorpyrifos was chosen as an appropriate member of the pesticide family. The result is that bisquaternary reactivators with two oxime groups in position four at the pyridinium rings...
Enantiospecific pharmacokinetic studies on ketoprofen in tablet formulation using indirect chiral HPLC analysis
Kannappan Valliappan, Kamarajan Kannan, Thanikachalam Sivakumar, Rajappan Manavalan
J Appl Biomed 4:153-161, 2006 | DOI: 10.32725/jab.2006.017
The present study was undertaken to evaluate the possibility of chiral discrimination in the release of enantiomers of ketoprofen (KT) in tablet form and, in turn, the bioavailability of the individual enantiomers, using the rabbit as a model. The enantiomeric concentration of KT in plasma was determined using a customized chiral HPLC analysis. First the plasma concentration-time profile was established for pure (±) KT in order to assess the extent of chiral discrimination. Subsequently the tablet formulation (Rhofenid-100mg) was administered, at a dose equivalent to 10 mg/kg, to assess the enantiospecific bioavailability of KT enantiomers in...
Reviews
Current progress in chronohaematology
Josef Berger
J Appl Biomed 4:111-114, 2006 | DOI: 10.32725/jab.2006.011
Cyclic changes play an important part of the space structure of an organism. Knowledge of the rhythms in haematological characteristics is important for both laboratory medicine and comparative pathology. New findings in chronohaematology published during the last two years are discussed. It seems that the haemato-immune circadian system reflects a circadian clock which is partially independent on the circadian clock in suprachiasmatic nuclei.