J Appl Biomed 5:139-150, 2007 | DOI: 10.32725/jab.2007.019

Chondrogenic differentiation of human bone marrow and adipose tissue-derived mesenchymal stem cells

Ľuboš Danišovič1,2,3, Petr Lesný1,2, Vojtěch Havlas4, Petr Teyssler4, Zdeňka Syrová1,2, Martin Kopáni5, Gabriela Fujeríková5, Tomáš Trč4, Eva Syková1,2, Pavla Jendelová1,2,*
1 Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2 Center for Cell Therapy and Tissue Repair, Second Medical Faculty, Charles University, Prague, Czech Republic
3 Institute of Medical Biology and Genetics, Comenius University, Faculty of Medicine, Bratislava, Slovak Republic
4 Department of Orthopaedic Surgery, Charles University, Second Medical Faculty, Prague, Czech Republic
5 Institute of Pathological Anatomy, Comenius University, Faculty of Medicine, Bratislava, Slovak Republic

Congenital abnormalities, various diseases and injuries may result in the degeneration of articular cartilage. Recently, stem cell therapy has offered new treatment possibilities for this condition. The aim of our study was to verify the chondrogenic differentiation potential of human bone marrow mesenchymal stem cells (BMSCs) and adipose tissue-derived mesenchymal stem cells (AMSCs) in vitro in the presence or absence of transforming growth factor beta (TGF-β1). Human BMSCs and AMSCs from healthy donors were collected during orthopaedic surgeries and expanded in vitro to obtain a sufficient quantity of cells; their chondrogenic differentiation was studied in the pellet culture system. Spontaneous chondrogenesis occurred in both BMSC and AMSC pellet cultures and was similar in both TGF-β1 treated and untreated pellet cultures. BMSC pellets contained more cells with a chondrogenic phenotype. The presence of TGF-β1 led to a decrease in the levels of collagen type I mRNA and to increased levels of collagen type II mRNA only in the BMSC pellet culture. Our results demonstrate that although both mesenchymal cell types can be used in cartilage tissue engineering, the chondrogenic potential of human BMSCs is higher than that of AMSCs.

Keywords: cartilage; collagen mRNA; mesenchymal stem cell; pellet cell culture; TGF-β1

Received: March 21, 2007; Revised: April 23, 2007; Published: July 31, 2007  Show citation

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Danišovič Ľ, Lesný P, Havlas V, Teyssler P, Syrová Z, Kopáni M, et al.. Chondrogenic differentiation of human bone marrow and adipose tissue-derived mesenchymal stem cells. J Appl Biomed. 2007;5(3):139-150. doi: 10.32725/jab.2007.019.
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