Journal of Applied Biomedicine, 2007 (vol. 5), issue 3
Original Research Article
The role of a mixture of green tea, turmeric and chitosan in the treatment of obesity-related testicular disorders
Mohamed El-Sweedy, Nabil Abdel-Hamid, Mohamed El-Moselhy
J Appl Biomed 5:131-138, 2007 | DOI: 10.32725/jab.2007.018 
In the present investigation, we studied the effect of aqueous green tea extract (GTE), alcoholic turmeric extract (ATE), and water-soluble Chitosan (WSC), individually/or in mixture, on the testicular tissue content of total cholesterol (TC), triglycerides (TG), phospholipids (PL), and thiobarbituric acid reactive substance (TBARS), in addition to nitric oxide (NO) in obese rats.The testicular weight of the obese rats was increased more significantly than control; TC, TG, PL, TBARS and NO were significantly higher in the obese group. GTE reduced testicular weight and significantly reduced other estimated parameter. ATE significantly increased...
Chondrogenic differentiation of human bone marrow and adipose tissue-derived mesenchymal stem cells
Ľuboš Danišovič, Petr Lesný, Vojtěch Havlas, Petr Teyssler, Zdeňka Syrová, Martin Kopáni, Gabriela Fujeríková, Tomáš Trč, Eva Syková, Pavla Jendelová
J Appl Biomed 5:139-150, 2007 | DOI: 10.32725/jab.2007.019
Congenital abnormalities, various diseases and injuries may result in the degeneration of articular cartilage. Recently, stem cell therapy has offered new treatment possibilities for this condition. The aim of our study was to verify the chondrogenic differentiation potential of human bone marrow mesenchymal stem cells (BMSCs) and adipose tissue-derived mesenchymal stem cells (AMSCs) in vitro in the presence or absence of transforming growth factor beta (TGF-β1). Human BMSCs and AMSCs from healthy donors were collected during orthopaedic surgeries and expanded in vitro to obtain a sufficient quantity of cells; their chondrogenic...
Interaction of aqueous extract of Pleurotus pulmonarius (Fr.) Quel.-Champ with acarbose in alloxan induced diabetic mice
Sachin L. Badole, Subhash L. Bodhankar
J Appl Biomed 5:157-166, 2007 | DOI: 10.32725/jab.2007.021
Mushrooms are a low calorie food with very little fat and are highly suitable for obese persons. The objective of the present investigation was to study the interaction of aqueous extract of P. pulmonarius (called PP-aqu) with acarbose on serum glucose levels, and on the oral glucose tolerance test (OGTT) in alloxan induced diabetic mice. PP-aqu (500 mg/kg), acarbose (50 mg/kg) and their combination were administered orally in alloxan (70 mg/kg i.v.) induced diabetic mice. In the acute study, the serum glucose level was estimated at 0, 2, 4, 6 and 24 h after drug administration. The subacute study involved repeated administration of the drugs...
Kinetics of decompositition of organophosphate Fenitrothion by decontaminating foam-making blends
Jiří Cabal, Kamil Kuča, Jitka Míčová
J Appl Biomed 5:167-170, 2007 | DOI: 10.32725/jab.2007.022
The hydrolytic efficacy of foam-making blends on the basis of cationic and nonionic surfactants and hydrogen peroxide was tested against the organophosphate Fenitrothion. The length of alkyl chains in both classes of surfactants and their mutual ratios are not parameters which significantly influenced the reactivity of blends. On the other hand, an increase in the hydrogen peroxide concentration induced a significant increase in the velocity of hydrolysis of Fenitrothion.
Reviews
Development of cardiac conduction system in mammals with a focus on the anatomical, functional and medical/genetical aspects
David Sedmera
J Appl Biomed 5:115-123, 2007 | DOI: 10.32725/jab.2007.016
Recent progress in search of mechanisms regulating the spatio-temporal formation of specialized conduction tissues pushes us further in our understanding of the developmental molecular mechanisms of myocardial patterning. It is not clear which mechanisms direct the fates of chamber myocytes to differentiate into ventricular cardiac conduction system (CCS). Similarly, the formation and nature of connections between its most distal component, the Purkinje fibers, and the working myocytes, is unresolved. The process of remodelling of originally ring-like AV junction into AV node and accompanying fibrous insulation between the atria and ventricles is poorly...
Phenylhydrazine haematotoxicity
Josef Berger
J Appl Biomed 5:125-130, 2007 | DOI: 10.32725/jab.2007.017
Phenylhydrazine (PHZ) and its derivatives were first given a medical application at the end of the 19th century but with very little benefit. However, this compound seems to be very useful in models studying mechanisms of haemolytic anaemia. Phenylhydrazine induces a reactive oxygen species formation, peroxidation of lipids and oxidative degradation of spectrin in the membrane skeleton. PHZ-induced haemolytic injury seems to be derived from oxidative alternations to red blood cell proteins. This compound can modulate immune reactions.
New methods for space reconstruction of inside cell structures
Dalibor Martišek, Karel Martišek, Jana Procházková
J Appl Biomed 5:151-156, 2007 | DOI: 10.32725/jab.2007.020
This paper discusses new methods for 3-D processing of confocal microscope outputs - single optical cuts through an explored object. The method can model cell illumination by an external light source and is a next important step in the realistic displaying of cells. The computer program based on these methods can be run on a common PC.