J Appl Biomed 6:27-30, 2008 | DOI: 10.32725/jab.2008.004

Optimization of acetylcholinesterase immobilization onto screen printed platinum electrode

Miroslav Pohanka1,*, Kamil Kuča2,3, Daniel Jun2,3
1 Centre of Biological Defense, Central Military Institute of Health, Těchonín, Czech Republic
2 Centre of Advanced Studies, Faculty of Military Medicine, University of Defense, Hradec Králové, Czech Republic
3 Department of Toxicology, Faculty of Military Medicine, University of Defense, Hradec Králové, Czech Republic

This article is focused on an optimization of acetylcholinesterase immobilization on screen printed platinum electrodes. An acetylcholinesterase layer cross linked by glutaraldehyde and another non cross linked were compared according to several parameters including background current, current before and current after inhibition by paraoxon. The percentage of inhibition was also calculated. The results obtained confirmed the importance of glutaraldehyde cross linking in the design of the acetylcholinesterase based biosensor.

Keywords: acetylcholinesterase; immobilization; cross linking; biosensor; electrochemical

Received: October 8, 2007; Revised: November 1, 2007; Published: March 31, 2008  Show citation

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Pohanka M, Kuča K, Jun D. Optimization of acetylcholinesterase immobilization onto screen printed platinum electrode. J Appl Biomed. 2008;6(1):27-30. doi: 10.32725/jab.2008.004.
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