Journal of Applied Biomedicine, 2008 (vol. 6), issue 1
Original Research Article
Detection of microsatellite instability in Czech HNPCC patients
Martina Sekowská, Anna Křepelová, Věra Kebrdlová
J Appl Biomed 6:15-18, 2008 | DOI: 10.32725/jab.2008.002 
The detection of microsatellite instability (MSI) is a standard part of mutational analysis in hereditary nonpolyposis colorectal cancers (HNPCC). A characteristic phenotypic feature of MSI indicates loss of mismatch repair (MMR) in tumour cells.We studied MSI in 205 tumours from 152 patients with HNPCC. Of these, 37 patients fulfilled the Amsterdam criteria, 72 patients were familial and 43 were sporadic cases. We used the method of fragmentation analysis (ABI Prism 310 Genetic Analyzer) with fluorescent labelled primers; three mononucleotide (BAT-RII, BAT-25, BAT-26) and five dinucleotide (D2S123, D3S1029, D5S346, D17S250, D18S58) repeat loci...
Antihyperglycaemic effect of Cardiospermum halicacabum Linn. leaf extract on STZ-induced diabetic rats
Chinnadurai Veeramani, Ganesan Pushpavalli, Kodukkur Viswanathan Pugalendi
J Appl Biomed 6:19-26, 2008 | DOI: 10.32725/jab.2008.003
The present study was designed to investigate the antihyperglycaemic effect of ethanolic extract of Cardiospermum halicacabum Linn. (Sapindaceae) leaves on normal and streptozotocin (STZ) diabetic rats. Diabetes was induced into male albino Wistar rats by intraperitonial administration of STZ. The Cardiospermum halicacabum leaf extract (CHE) was administered orally at three different doses to normal and STZ-diabetic rats for 45 days. The diabetic rats showed an increase in levels of blood glucose and glycosylated haemoglobin (HbA1c) and a decrease in the levels of insulin and haemoglobin (Hb). In addition, diabetic rats showed...
Optimization of acetylcholinesterase immobilization onto screen printed platinum electrode
Miroslav Pohanka, Kamil Kuča, Daniel Jun
J Appl Biomed 6:27-30, 2008 | DOI: 10.32725/jab.2008.004
This article is focused on an optimization of acetylcholinesterase immobilization on screen printed platinum electrodes. An acetylcholinesterase layer cross linked by glutaraldehyde and another non cross linked were compared according to several parameters including background current, current before and current after inhibition by paraoxon. The percentage of inhibition was also calculated. The results obtained confirmed the importance of glutaraldehyde cross linking in the design of the acetylcholinesterase based biosensor.
Effect of pterostilbene on lipids and lipid profiles in streptozotocin-nicotinamide induced type 2 diabetes mellitus
Marudamuthu Amarnath Satheesh, Leelavinothan Pari
J Appl Biomed 6:31-37, 2008 | DOI: 10.32725/jab.2008.005
Hyperlipidaemia is one of the major risk factors of cardiovascular complication in diabetes. A study was undertaken to evaluate the antihyperlipidaemic activity of pterostilbene. Oral administration of pterostilbene (40mg/kg bodyweight) to streptozotocin-nicotinamide induced diabetic rats for 6 weeks significantly reduced the elevated serum very low density lipoprotein (VLDL) and low density lipoprotein (LDL)-cholesterol levels and significantly increased the serum high-density lipoprotein (HDL)-cholesterol level. In addition, pterostilbene also significantly lowered the levels of triglycerides, phospholipids, free fatty acids and total cholesterol...
Steady-state bioequivalence studies of two memantine tablet and oral solution formulations in healthy volunteers
Jaroslav Chládek, Bořek Žaludek, Petr Sova, Aleš Franc, Luděk Šišpera, Jiřina Martínková, Stanislav Mičuda, Jolana Cermanová
J Appl Biomed 6:39-45, 2008 | DOI: 10.32725/jab.2008.006
The bioavailability of memantine was compared using two tablet (Memantine LACHEMA 10 tbl. obd. and Akatinol® Memantine 10 tbl. obd., Study A) and two oral solution formulations (Memantine LACHEMA gtt. and Akatinol® Memantine gtt., Study B) containing 10 mg memantine hydrochloride in two randomized, two-period, two-sequence, crossover studies with 24 healthy volunteers. In both study periods, memantine concentrations were determined by gas-chromatography with electron-capture detection in plasma samples taken at the steady state after 22 days of once-daily dosing. The arithmetic mean (SD) pharmacokinetic parameters in the studies A and B were:...
Reviews
Plaque imaging: clinical implications and use of invasive methods
Stanislav Tůma, David Tesař, Josef Veselka, Jiří Neuwirth
J Appl Biomed 6:1-14, 2008 | DOI: 10.32725/jab.2008.001
The composition of artherosclerotic plaque can be distingushed from the normal physical properties of tissues by the inflammatory mediators and the cells' remodelling arterial walls, and can therefore be demonstrated by imaging methods. Imaging of the vulnerable atherosclerotic plaque is now accepted as the main target in the diagnostics of imminent acute complications in atherosclerosis. Recent results of clinical trials (Ross's, CAPTIM, Prague) suggest facilitated intervention - primary angioplasty together with pre-hospital fibrinolysis during transportation - as the urgent therapeutical method of choice. This life-saving emergency treatment is...