J Appl Biomed 7:143-149, 2009 | DOI: 10.32725/jab.2009.016

Could oxime HI-6 really be considered as "broad-spectrum" antidote?

Kamil Kuča1,2,*, Kamil Musílek2, Daniel Jun1,2,3, Miroslav Pohanka1, Jana ®ďárová Karasová2, Ladislav Novotný1, Lucie Musilová4
1 Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic
2 Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic
3 Department of Water Resources and Environmental Modeling, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Prague, Czech Republic
4 Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Hradec Králové, Czech Republic

The broad-spectrum reactivator is a valuable oxime able to reactivate acetylcholinesterase (AChE) inhibited by nerve agents and pesticides. At present there are many AChE reactivators (oximes) which are suitable candidates as broad-spectrum reactivators and among them is the oxime HI-6, highly enough thought of to have been recommended by many armies for use as a universal antidote. In this study, we wanted to establish whether the designation "broad-spectrum" is a an accurate description or if there are some lacks in reactivation of nerve agents or pesticides. For this purpose, the general in vitro test for the evaluation of AChE reactivators was used. Tabun, sarin, cyclosarin, soman, VX agent, Russian VX were used as nerve agents for testing, and chlorpyrifos, paraoxon, methyl-chlorpyrifos and dichlorvos (DDVP) were used as typical examples of organophoshorus pesticides. The results obtained showed that oxime HI-6 did not reactivate tabun- and DDVP-inhibited AChE, and, in the case of the other pesticides, only a high dose of oxime HI-6 was able to reactivate pesticide-inhibited AChE.

Keywords: acetylcholinesterase; reactivator; oxime; nerve agent; pesticide; HI-6; broad-spectrum

Received: June 16, 2009; Revised: July 16, 2009; Published: July 31, 2009  Show citation

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Kuča K, Musílek K, Jun D, Pohanka M, ®ďárová Karasová J, Novotný L, Musilová L. Could oxime HI-6 really be considered as "broad-spectrum" antidote? J Appl Biomed. 2009;7(3):143-149. doi: 10.32725/jab.2009.016.
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