J Appl Biomed 7:201-206, 2009 | DOI: 10.32725/jab.2009.022

Inhibition of blood cholinesterases by nerve agents in vitro

Jiří Bajgar1,*, Daniel Jun1,2, Kamil Kuča1,2, Josef Fusek1, Jana ®ďárová Karasová1, Jiří Kassa1, Jiří Cabal1, Václav Bláha3
1 Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic
2 Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic
3 Department of Multidisciplinary Studies, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic

The bimolecular rate constants (ka) of inhibition were determined for cholinesterase in rat blood in vivo and three nerve agents in vitro. They were in the range of 105-106 M.min -1 with the highest value for VX (ka = 8.92 × 106), the next highest for soman (ka = 3.22 × 106) and the lowest for sarin (ka = 0.39 × 106), respectively. The inhibition rate is the same in vitro and in vivo; when the real inhibition in vivo and in vitro is compared, it is possible to assess the concentration of the nerve agent present in the blood stream. When administered intramuscularly it is about 70% of the dose administered.

Keywords: whole blood cholinesterases; rat; nerve agents; inhibition in vitro

Received: June 17, 2009; Revised: September 8, 2009; Published: July 31, 2009  Show citation

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Bajgar J, Jun D, Kuča K, Fusek J, ®ďárová Karasová J, Kassa J, et al.. Inhibition of blood cholinesterases by nerve agents in vitro. J Appl Biomed. 2009;7(4):201-206. doi: 10.32725/jab.2009.022.
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