J Appl Biomed 9:111-118, 2011 | DOI: 10.2478/v10136-009-0036-2

Ectopic osteogenesis with immortalized human bone marrow stromal stem cells and heterologous bone

Yong Teng^1,*, Yunyu Hu², Xusheng Li¹, Yucheng Guan¹, Junhao Gui¹

¹ No. 1 Department of Orthopedics, Orthopedic Center of PLA, Urumqi General Hospital, Lanzhou Military Region, Xinjiang Uygur Autonomous Region 830000, China

² Orthopaedic Institute, Affiliated Xijing Hospital of the Fourth Military Medical University, Xi'an710032, China

To resolve the problem of the insufficient availability of seed cells and to provide seed cells for tissue engineering research, an immortalized human bone marrow stromal stem cell line (MSCxj cells) was established in our department to investigate the ectopic osteogenesis of MSCxj cells.
MSCxjs were grown with a heterogeneous bone scaffold for 48 h. Three groups were included: group A: MSCxjs of 35 PDs were maintained with heterogeneous bone; group B: MSCxjs of 128 PDs were maintained with heterogeneous bone; and group C: heterogeneous bone alone. Tetracycline fluorescence staining, H&E staining, and ponceau staining, immunohistochemistry and bone histomorphometry were performed. At the same time, scanning electron microscopy was conducted to detect the growth of MSCxjs and heterogeneous bone.
Scanning electron microscopy showed favorable adherence of MSCxjs to heterogeneous bone. A large number of newly generated filamentous extracellular matrix and fine granular materials were found to cover the cells. The results from staining showed that the osteogenesis was not obvious in group A/B 4 weeks after transplantation. Eight weeks after implantation, osteoid matrix deposition was noted in and around the heterogeneous bone in group A/B. Twelve weeks after implantation, osteogenesis was increased in group A/B. There were no significant differences in the time course for bone formation and the amount of newly generated bone between group A/B.
Like primary hBMSCs, MSCxj cells have favourable ectopic osteogenesis and can be applied as seeded cells in bone tissue engineering.

Keywords: tissue engineering; immortalization; human; bone marrow stromal cells; ectopic osteogenesis; xenografts

Received: October 18, 2010; Published: July 31, 2011  Show citation