Journal of Applied Biomedicine, 2011 (vol. 9), issue 2

Original Research Article

Square wave voltammetry on screen printed electrodes: comparison to ferric reducing antioxidant power in plasma from model laboratory animal (Grey Partridge) and comparison to standard antioxidants

Miroslav Pohanka, Hana Banďouchová, Kristina Vlčková, Jana Žďárová Karasová, Kamil Kuča, Veronika Damková, Lucie Pecková, František Vitula, Jiří Pikula

J Appl Biomed 9:103-109, 2011 | DOI: 10.2478/v10136-009-0032-6  

Low molecular weight antioxidants (LMWAs) were assayed by square wave voltammetry (SWV) using screen printed electrodes. Standard antioxidants, i.e. uric acid, ascorbic acid, trolox and glutathione, were assayed in order to estimate the sensitivity and standard redox potentials of individual LMWAs. In another experiment, plasma from Grey Partridges was used as model real samples. Ferric reducing antioxidant power (FRAP) was used as a reference method. Two peaks in plasma samples were found by SWV and correlated to FRAP. The SWV peaks were successfully correlated to FRAP. The practical importance of SWV carried out on screen printed electrodes is discussed.

Ectopic osteogenesis with immortalized human bone marrow stromal stem cells and heterologous bone

Yong Teng, Yunyu Hu, Xusheng Li, Yucheng Guan, Junhao Gui

J Appl Biomed 9:111-118, 2011 | DOI: 10.2478/v10136-009-0036-2  

To resolve the problem of the insufficient availability of seed cells and to provide seed cells for tissue engineering research, an immortalized human bone marrow stromal stem cell line (MSCxj cells) was established in our department to investigate the ectopic osteogenesis of MSCxj cells.MSCxjs were grown with a heterogeneous bone scaffold for 48 h. Three groups were included: group A: MSCxjs of 35 PDs were maintained with heterogeneous bone; group B: MSCxjs of 128 PDs were maintained with heterogeneous bone; and group C: heterogeneous bone alone. Tetracycline fluorescence staining, H&E staining, and ponceau staining, immunohistochemistry and...

Reviews

The age of biomedicine: current trends in traditional subjects

Josef Berger

J Appl Biomed 9:57-61, 2011 | DOI: 10.2478/v10136-011-0004-5  

The earliest scientific journals on biomedicine began publication in the 50s and their authors addressed the application of biology to medicine. More recently, biochemistry and biomedical engineering questions have figured more prominently. This trend is discussed in a survey of the topics appearing in the Journal of Applied Biomedicine. Pharmacological and toxicological articles have been popular over the long term and the neurosciences, chronomedicine, molecular and cell biomedicine have also been very important. The role of computational biomedicine and nanomedicine has received increasing attention as has the part which applied biomedicine can...

Congruent biospheric and solar-terrestrial cycles

Germaine Cornélissen, Patricia Grambsch, Robert Bruce Sothern, George Katinas, Kuniaki Otsuka, Franz Halberg

J Appl Biomed 9:63-102, 2011 | DOI: 10.2478/v10136-009-0023-7  

Selective congruence, namely a pairing of various biospheric cycles of certain frequencies with different environmental ones and further selectivity of phase behavior at the given frequency characterize an ultradian to infradian, prominently circadian transdisciplinary spectrum. Diseases documented among others to be influenced by the cosmos range from individuals' strokes to populations' crime and terrorism, conditions studied by chronomics as time structures (chronomes). Methods of investigation include the extended cosinor allowing for the added estimation of the period with a measure of uncertainty, as well as global and gliding spectral windows...