J Appl Biomed 9:129-141, 2011 | DOI: 10.2478/v10136-011-0001-8

Effects of SDF-1α/CXCR4 on vascular smooth muscle cells and bone marrow mesenchymal cells in a rat carotid artery balloon injury model

Wen-Wei Cai1, Ning-Yuan Fang2,*, Jing Sheng1, Shao-Jun Ma1, Zhi-Hui Chen1
1 Department of Geriatrics, 9th Hospital, Shanghai Jiao-Tong University School of Medicine (SJTU-SM), Shanghai 200011, China
2 Department of Geriatrics, Ren-Ji Hospital, SJTU-SM, Shanghai 200001, China

Bone marrow mensenchyme cells (BMSCs) can differentiate into endothelial progenitor cells which then migrate to injured sites for the repair of neointima, and stromal cell-derived factor-1α (SDF-1α) can mediate the migration of CXCR4 expressing stem/progenitor cells to injured sites for pair. Protein and mRNA expression of SDF-1α and CXCR4 were determined by RT-PCR, Western blot and ELISA. Immediately after common carotid artery balloon injury, the mRNA expression of SDF-1α in vascular smooth muscle cells (VSMCs) first increased and then decreased 7 days later. VSMCs transfected with SDF-1α siRNA did not express SDF-1α mRNA, but after transfection with SDF-1α siRNA, the SDF-1α content in injured VSMCs gradually returned to the baseline level. Normal BMSCs rarely expressed CXCR4 mRNA, but the CXCR4 mRNA expression on BMSCs increased significantly 4 days after common carotid artery injury and was maintained. The migration of BMSCs after artery injury was enhanced when compared with normal BMSCs, but SDF-1α siRNA transfection of VSMCs and AMD3100 treatment remarkably decreased the chemotaxis of BMSCs to VSMCs and SDF-1α, respectively. We conclude that the SDF-1α/CXCR4 axis plays an important role in the migration of BMSCs after balloon injury and can ultimately cause abnormal proliferation of the intima.

Keywords: bone marrow mensenchyme cells; vascular smooth muscle cells; stromal cell-derived factor-1α

Received: December 20, 2010; Revised: February 10, 2011; Published: July 31, 2011  Show citation

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Cai W, Fang N, Sheng J, Ma S, Chen Z. Effects of SDF-1α/CXCR4 on vascular smooth muscle cells and bone marrow mesenchymal cells in a rat carotid artery balloon injury model. J Appl Biomed. 2011;9(3):129-141. doi: 10.2478/v10136-011-0001-8.
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