J Appl Biomed 13:123-129, 2015 | DOI: 10.1016/j.jab.2014.08.001

A new gold nanoparticle based rapid immunochromatographic assay for screening EBV-VCA specific IgA in nasopharyngeal carcinomas

Saeid Goudarzia, Anita Ahmadib,c, Mohammad Farhadia, Seyed Kamran Kamravaa, Fariborz Mobarrezd, Kobra Omidfarb,c,*
a Clinical Nanomedicine Laboratory, ENT-Head & Neck Research Center, Hazrate Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran
b Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran
c Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran
d Karolinska Institutet, Department of Medicine Solna, Unit of Rheumatology, Karolinska University Hospital, Stockholm, Sweden

Large screening programs of nasopharyngeal carcinoma (NPC) are still challenging due to the scarcity of the resources in higher prevalence parts of the world. In the present study, the development of a new low cost immunochromatography (ICG) strip test for screening of nasopharyngeal carcinoma is reported. A conjugate of gold nanoparticle-anti viral capsid antigen (VCA) immunoglobulin A (IgA) antibody was used as the detection probe. The test is based on a non-competitive immunoassay for rapid and reliable detection the serum specific IgA of Epstein-Barr virus (EBV) VCA. Patient serum samples were analyzed with the strip and compared with enzyme-linked immunosorbent assay (ELISA) and pathological evaluation. The obtained results from the ICG strips were well correlated with the two reference methods. An evaluation of stability, sensitivity and specificity suggested the strip suitable for NPC screening programs in high-risk peoples and also for the recurrence monitoring.

Keywords: Cancer biomarker; EBV-VCA IgA; Gold nanoparticle; Nasopharyngeal carcinoma; Immunochromatography; Screening test

Received: June 28, 2014; Revised: August 13, 2014; Accepted: August 18, 2014; Published: May 1, 2015  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Goudarzi S, Ahmadi A, Farhadi M, Kamran Kamrava S, Mobarrez F, Omidfar K. A new gold nanoparticle based rapid immunochromatographic assay for screening EBV-VCA specific IgA in nasopharyngeal carcinomas. J Appl Biomed. 2015;13(2):123-129. doi: 10.1016/j.jab.2014.08.001.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Cao, S.M., Liu1, Z., Jia, W.H., et al., 2011. Fluctuations of Epstein- Barr virus serological antibodies and risk for nasopharyngeal carcinoma: a prospective screening study with a 20-year follow-up. PLoS One 22, e19100. Go to original source...
    2. Chang, E.T., Adami, H.O., 2006. The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer Epidemiol. Biomarkers Prev. 15, 1765-1777. Go to original source... Go to PubMed...
    3. Chen, J.Y., Chen, C.J., Liu, M.Y., et al., 1989. Antibody to Epstein- Barr virus-specific DNase as a marker for field survey of patients with nasopharyngeal carcinoma in Taiwan. J. Med. Virol. 27, 269-273. Go to original source... Go to PubMed...
    4. Chien, Y.C., Chen, J.Y., Liu, M.Y., et al., 2001. Serologic markers of Epstein-Barr virus infection and nasopharyngeal carcinoma in Taiwanese men. N. Engl. J. Med. 345, 1877-1882. Go to original source... Go to PubMed...
    5. Chi-shing Cho, W., 2007. Nasopharyngeal carcinoma: molecular biomarker discovery and progress. Mol. Cancer 6, 1. Go to original source... Go to PubMed...
    6. Deng, H., Zhao, Z., Zhang, Z., 1995. Serologic screening on nasopharyngeal cancer in 338,868 persons in 21 cities and counties of Guangxi Region, China. Zhonghua Yu Fang Yi Xue Za Zhi 29, 342-343. Go to PubMed...
    7. Fachiroh, J., Paramita, D.K., Hariwiyanto, B., et al., 2006. Singleassay combination of Epstein-Barr virus (EBV) EBNA-1 and viral capsid antigen-p18-derived synthetic peptides for measuring anti-EBV immunoglobulin G (IgG) and IgA antibody levels in sera from nasopharyngeal carcinoma patients: options for field screening. J. Clin. Microbiol. 44, 1459-1467. Go to original source... Go to PubMed...
    8. Gui, W.J., Wang, S.T., Guo, Y.R., Zhu, G.N., 2008. Development of a one-step strip for the detection of triazophos residues in environmental samples. Anal. Biochem. 377, 202-208. Go to original source... Go to PubMed...
    9. Henle, G., Henle, W., 1976. Epstein-Barr virus-specific IgA serum antibodies as an outstanding feature of nasopharyngeal carcinoma. Int. J. Cancer 17, 1-7. Go to original source... Go to PubMed...
    10. Henle, W., Ho, H.C., Henle, G., Kwan, H.C., 1973. Antibodies to Epstein-Barr virus-related antigens in nasopharyngeal carcinoma. Comparison of active cases with long-term survivors. J. Natl. Cancer Inst. 51, 361-369. Go to original source... Go to PubMed...
    11. Ho, D.W., Yang, Z.F., Wong, B.Y., et al., 2006. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry serum protein profiling to identify nasopharyngeal carcinoma. Cancer 107, 99-107. Go to original source... Go to PubMed...
    12. Jemal, A., Bray, F., Center, M., Ferlay, J., Ward, E., Forman, D., 2011. Global cancer Statistics. CA Cancer J. Clin. 61, 69-90. Go to original source... Go to PubMed...
    13. Jiang, L.N., Dai, L.C., He, J.F., Chen, Y.W., Ma, Z.H., 2006. Significance of detection of serum sialic acid and Epstein-Barr virus VCA-IgA in diagnosis and monitoring radiotherapy effectiveness in nasopharyngeal carcinoma patients. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 20, 30-32.
    14. Kao, C.H., Shiau, Y.U.C., Shen, Y.Y., Yen, R.F., 2002. Detection of recurrent or persistent nasopharyngeal carcinomas after radiotherapy with technetium-99m methoxyisobutylisonitrile single photon emission computed tomography and computed tomography: comparison with 18-fluoro-2-deoxyglucose positron emission tomography. Chin. J. Cancer 94, 1981-1986. Go to original source... Go to PubMed...
    15. King, A.D., Vlantis, A.C., Bhatia, K.S.S., et al., 2011. Primary nasopharyngeal carcinoma: diagnostic accuracy of MR imaging versus that of endoscopy and endoscopic biopsy. Radiology 258, 531-537. Go to original source... Go to PubMed...
    16. Lee, E.Y., Kang, J.H., Kim, K.A., et al., 2006. Development of a rapid immunochromatographic strip test for serum asialo alpha1acid glycoprotein in patients with hepatic disease. J. Immunol. Methods 308, 116-123. Go to original source... Go to PubMed...
    17. Lo, Y.M., Chan, L.Y., Lo, K.W., et al., 1999. Quantitative analysis of cell-free Epstein-Barr virus DNA in plasma of patients with nasopharyngeal cancer. Cancer Res. 59, 1188-1191.
    18. Low, W.K., Leong, J.L., Goh, Y.H., Fong, K.W., 2000. Diagnostic value of Epstein-Barr viral serology in nasopharyngeal carcinoma. Otolaryngol. Head Neck Surg. 123, 505-507. Go to original source... Go to PubMed...
    19. Lynn, T.C., Hsieh, R.P., Chuang, C.Y., Huang, S.C., Hsieh, T., Tu, S. M., 1984. Epstein-Barr virus-associated antibodies and serum biochemistry in nasopharyngeal carcinoma. Laryngoscope 94, 1485-1488. Go to original source... Go to PubMed...
    20. Molinelli, A., Grossalber, K., Führer, M., Baumgartner, S., Sulyok, M., Krska, R., 2008. Development of qualitative and semiquantitative immunoassay-based rapid strip tests for the detection of T-2 toxin in wheat and oat. J. Agric. Food Chem. 56, 2589-2594. Go to original source... Go to PubMed...
    21. Mousavi, S.M., 2006. National Cancer Registry Report 2005-2006. Ministry of Health, Deputy for Health Directory, CDC Cancer Office, Tehran, Iran (Persian).
    22. Nagatani, N., Yuhi, T., Chikae, M., et al., 2006. A sensitive immunochromatographic assay using gold nanoparticles for the semiquantitative detection of prostate-specific antigen in serum. Nanobiotechnology 2, 79-86. Go to original source...
    23. Neel III, H.B., Pearson, G.R., Weiland, L.H., et al., 1983. Application of Epstein-Barr virus serology to the diagnosis and staging of North American patients with nasopharyngeal carcinoma. Otolaryngol. Head Neck Surg. 91, 255-262. Go to original source... Go to PubMed...
    24. Neel III, H.B., Pearson, G.R., Taylor, W.F., 1984. Antibodies to Epstein-Barr virus in patients with nasopharyngeal carcinoma and in comparison groups. Ann. Otol. Rhinol. Laryngol. 93, 477-482. Go to original source... Go to PubMed...
    25. Omidfar, K., Kia, S., Kashanian, S., et al., 2010. Colloidal nanogold-based immunochromatographic strip test for the detection of digoxin toxicity. Appl. Biochem. Biotechnol. 160, 843-855. Go to original source... Go to PubMed...
    26. Omidfar, K., Kia, S., Larijani, B., 2011. Development of a colloidal gold-based immunochromatographic test strip for screening of microalbuminuria. Hybridoma 30, 117-124. Go to original source... Go to PubMed...
    27. Omidfar, K., Khorsand, B., Larijani, B., 2012a. Development of a new sensitive immunostrip assay based on mesoporous silica and colloidal Au nanoparticles. Mol. Biol. Rep. 39, 1253-1259. Go to original source... Go to PubMed...
    28. Omidfar, K., Zarei, H., Gholizadeh, F., Larijani, B., 2012b. A highsensitivity electrochemical immunosensor based on mobile crystalline material-41-polyvinyl alcohol nanocomposite and colloidal gold nanoparticles. Anal. Biochem. 421, 649-656. Go to original source... Go to PubMed...
    29. Omidfar, K., Khorsand, F., Darziani Azizi, M., 2013. New analytical applications of gold nanoparticles as label in antibody based sensors. Biosens. Bioelectron. 26, 336-347. Go to original source... Go to PubMed...
    30. Parkin, D.M., Bray, F., Ferlay, J., Pisani, P., 2005. Global cancer statistics, 2002. CA Cancer J. Clin. 55, 74-108. Go to original source... Go to PubMed...
    31. Raah-Trauh, N., 1992. Epstein-Barr virus aiid nasopharyngeal carcinoma. Semin. Cancer Biol. 3, 297-307.
    32. Sacmundsen, A.K., Aiheck, H., Hansen, J.P.H., 1982. Epstein-Barr virus in nasopharyngeal attd salivary gland carcinomas in Greenland Eskimos. Br. J. Cancer 46, 721-728. Go to original source... Go to PubMed...
    33. Saika, K., Sobue, T., 2008. Comparison of time trends in cancer incidence (1973-2002) in Asia, from cancer incidence in five continents Vols IV-IX. Jpn. J. Clin. Oncol. 38, 872-873. Go to original source... Go to PubMed...
    34. Shanmugaratnam, K., Chan, S.H., de-The, G., et al., 1979. Histopathology of nasopharyngeal carcinoma. Correlations with epidemiology, survival rates and other biological characteristics. Cancer 44, 1029-1044. Go to original source...
    35. Shimakage, M., Ikegami, N., Chatani, M., Yoshino, K., Sato, T., 1987. Serological follow-up study on the antibody levels to Epstein-Barr virus-determined nuclear antigen (EBNA) patients with nasopharyngeal carcinoma (NPC) after radiation therapy. Biken J. 30, 45-51. Go to PubMed...
    36. Xiulan, S., Xiaolian, Z., Jian, T., Zhou, J., Chu, F., 2005. Preparation of gold-labeled antibody probe and its use in immunochromatography assay for detection of aflatoxin B1. Int. J. Food Microbiol. 99, 185-194. Go to original source... Go to PubMed...
    37. Yen, R.F., Hong, R.L., Tzen, K.Y., Pan, M.H., Chen, T.H.H., 2005. Whole-body 18F-FDG PET in recurrent or metastatic nasopharyngeal carcinoma. J. Nucl. Med. 46, 770-774. Go to PubMed...
    38. Yen, R.F., Hung, R.L., Pan, M.H., et al., 2003. 18-Fluoro-2deoxyglucose positron emission tomography in detecting residual/recurrent nasopharyngeal carcinomas and comparison with magnetic resonance imaging. Chin. J. Cancer 98, 283-287. Go to original source... Go to PubMed...
    39. Zeng, Y., Zhang, L.G., Li, H.Y., et al., 1982. Serological mass survey for early detection of nasopharyngeal carcinoma in Wuzhou City, China. Int. J. Cancer 29, 139-141. Go to original source... Go to PubMed...

    Return to the content