Journal of Applied Biomedicine, 2015 (vol. 13), issue 2

Contents

J Appl Biomed 13:v, 2015 | DOI: 10.1016/S1214-021X(15)00017-4  

Up-regulation of orexigenic and down-regulation of anorexigenic neuropeptide gene expression in rat hypothalamus after partial lipectomy

Agnieszka Dettlaff-Pokora, Tomasz Sledzinski, Julian Swierczynski

J Appl Biomed 13:105-112, 2015 | DOI: 10.1016/j.jab.2015.01.003  

Leptin down-regulates orexigenic and up-regulates anorexigenic neuropeptide gene expression in hypothalamus. Surgical removal of adipose tissue leads to decrease in circulating leptin concentrations in rats. In the present study, we tested: (a) regulation of neuropeptide gene expression in hypothalamus, (b) food intake, and (c) standard growth rate after removal of adipose tissue in rats. Partial lipectomy caused an approximately 10-fold reduction of subcutaneous, retroperitoneal and epididymal adipose tissue weight (at the end of experiments adipose tissue weight was 1.5 ± 0.9 in lipectomy and 15 ± 3.9 g in control rats; statistically...

Herbal product silibinin-induced programmed cell death is enhanced by metformin in cervical cancer cells at the dose without influence on nonmalignant cells

Wen-Shiung Liou, Li-Jen Chen, Ho-Shan Niu, Ting-Ting Yang, Juei-Tang Cheng, Kao-Chang Lin

J Appl Biomed 13:113-121, 2015 | DOI: 10.1016/j.jab.2014.11.001  

Silibinin is known to display high efficacy against cancer cells and for hepatic protection. Metformin, a well-known antidiabetic agent, has recently been reported to inhibit cancer. In the present study, we investigated the effect of metformin on silibinin-induced programmed cell death in cervical cancer cells (C-33A). MTT assay and Western blot assays were performed to quantify cell viability and the expression of signaling proteins, respectively. Combined treatment with metformin and silibinin decreased cell survival in synergistic manner in C-33A cells at a dose that did not affect nonmalignant cells (HUVECs). Silibinin and metformin increased...

A new gold nanoparticle based rapid immunochromatographic assay for screening EBV-VCA specific IgA in nasopharyngeal carcinomas

Saeid Goudarzi, Anita Ahmadi, Mohammad Farhadi, Seyed Kamran Kamrava, Fariborz Mobarrez, Kobra Omidfar

J Appl Biomed 13:123-129, 2015 | DOI: 10.1016/j.jab.2014.08.001  

Large screening programs of nasopharyngeal carcinoma (NPC) are still challenging due to the scarcity of the resources in higher prevalence parts of the world. In the present study, the development of a new low cost immunochromatography (ICG) strip test for screening of nasopharyngeal carcinoma is reported. A conjugate of gold nanoparticle-anti viral capsid antigen (VCA) immunoglobulin A (IgA) antibody was used as the detection probe. The test is based on a non-competitive immunoassay for rapid and reliable detection the serum specific IgA of Epstein-Barr virus (EBV) VCA. Patient serum samples were analyzed with the strip and compared with enzyme-linked...

Free radicals in ultraviolet irradiated melanins and melanin complexes with Cd(II) and Cu(II) - EPR examination

Magdalena Zdybel, Ewa Chodurek, Barbara Pilawa

J Appl Biomed 13:131-141, 2015 | DOI: 10.1016/j.jab.2015.01.002  

Effect of UV irradiation on free radicals in different types of melanins and melanin complexes with diamagnetic Cd(II) and paramagnetic Cu(II) was examined by the use of electron paramagnetic resonance (EPR) spectroscopy. The aim of this studies was to compare o-semiquinone free radicals formation in two model eumelanins synthesized from 3,4-dihydroxyphenylalanine (DOPA) and tyrosine in the presence of tyrosinase, and in synthetic pheomelanin, under exposition on ultraviolet, because of the important role of free radicals and melanins in human organism. UV may change free radical concentrations in melanin. Changes in EPR spectra of DOPA-melanin-Cd(II)...

Estimation of the spinal twisting angle using inertial measurement units during a rod derotation surgery in idiopathic scoliosis patients

Hyun Mu Heo, Seung Eel Oh, Seung Woo Suh, Jae Hyuk Yang, Su Hyun Youn, Taeyong Sim, Joung Hwan Mun

J Appl Biomed 13:143-159, 2015 | DOI: 10.1016/j.jab.2015.01.001  

This study proposes a method to measure the twisting angle of a rod during a spinal correction surgery in real-time without performing an alignment procedure by using the six-axis data (tri-axis acceleration, tri-axis rate gyro) of the IMU and a Tait-Bryan Euler angle algorithm. To determine whether the twisting angle calculation algorithm offered an improvement, typical procedures described in existing studies were implemented using the same experimental data and were then validated using a three-dimensional motion analysis system (Vicon 460 motion analysis system). The correlation coefficients and the RMSE of the proposed method were 0.904 and 0.680°...

Systemic oxidative stress in victims of Bothrops snakebites

Juliana de Ornellas Strapazzon, Eduardo Benedetti Parisotto, Ana Maria Moratelli, Thais Regina Garlet, Juliana Bastos, Ivan Ricardo Zimermann, Marlene Zanin, Rodrigo Fagundez, Manuel Rosa de Oliveira Lino, Tânia Silvia Fröde, Danilo Wilhelm Filho

J Appl Biomed 13:161-167, 2015 | DOI: 10.1016/j.jab.2014.11.002  

The genus Bothrops is responsible for approximately 90% of snakebites in Brazil. In the present study biomarkers of oxidative stress (OS) were evaluated in the blood of victims of snakebites from Bothrops jararaca and Bothrops jararacussu. Patient monitoring started from the emergency entrance at the hospital up to 30 days, groups divided as follows: time 0 (t₀), 24 hours (t_24h), 7 days (t_7d) and 30 days (t_30d). The activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), and myeloperoxidase (MPO),...

Multinuclear magnetic resonance evaluation of tumor tissue

Dorota Bartusik, David Aebisher

J Appl Biomed 13:69-78, 2015 | DOI: 10.1016/j.jab.2014.10.002  

Current biomedical research requires quantitative and qualitative studies of tissue metabolites. In this review, we discuss recent advances in multinuclear magnetic resonance (MR) technology that enhance biomedical research. The advances presented herein clearly show that MR is a diagnostic tool for the study of tumor tissue metabolism associated with changes in small molecule concentrations. Biomedical MR offers a non-invasive view of cancer cell metabolism by detection of resonance nuclei such as hydrogen-1, carbon-13, phosphorus-31 and fluorine-19. Due to current progress in MR technology, it is now possible to monitor changes in cancer cell metabolism...

Coordination compounds in cancer: Past, present and perspectives

Federica Trudu, Filippo Amato, Petr Vaňhara, Tiziana Pivetta, E.M. Peña-Méndez, Josef Havel

J Appl Biomed 13:79-103, 2015 | DOI: 10.1016/j.jab.2015.03.003  

Metal-based coordination compounds have been used throughout the history of human medicine to treat various diseases, including cancer. Since the discovery of cisplatin in 1965, a great number of metal coordination complexes, such as platinum, ruthenium, gold or copper have been designed, synthesized and tested in order to develop clinically effective and safe drugs. Currently, many reviews cover applications of cytostatic metal complexes pointing out the most promising examples of platinum- and non-platinum-based compounds in preclinical and clinical trials. However, recent comprehensive reviews covering chemical and biological aspects of metal-based...