J Appl Biomed 13:161-167, 2015 | DOI: 10.1016/j.jab.2014.11.002

Systemic oxidative stress in victims of Bothrops snakebites

Juliana de Ornellas Strapazzona, Eduardo Benedetti Parisottoa, Ana Maria Moratellia, Thais Regina Garleta, Juliana Bastosb, Ivan Ricardo Zimermannc, Marlene Zaninc, Rodrigo Fagundeza, Manuel Rosa de Oliveira Linod, Tânia Silvia Frödee, Danilo Wilhelm Filhoa,*
a Department of Ecology and Zoology, Federal University of Santa Catarina, Florianópolis, Brazil
b Department of Chemistry, Federal University of Santa Catarina, Florianópolis, Brazil
c Center for Toxicological Information, Federal University of Santa Catarina, Florianópolis, Brazil
d Department of Informatics and Statistics, Federal University of Santa Catarina, Florianópolis, Brazil
e Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil

The genus Bothrops is responsible for approximately 90% of snakebites in Brazil. In the present study biomarkers of oxidative stress (OS) were evaluated in the blood of victims of snakebites from Bothrops jararaca and Bothrops jararacussu. Patient monitoring started from the emergency entrance at the hospital up to 30 days, groups divided as follows: time 0 (t0), 24 hours (t24h), 7 days (t7d) and 30 days (t30d). The activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), and myeloperoxidase (MPO), as well as the contents of reduced glutathione (GSH), vitamin E, lipid peroxidation (TBARS), protein carbonyls (PC) were examined in blood. Initial determinations revealed increased CAT, GR and GPx activities and decreased SOD and GST activities together with the depletion of GSH contents, while markers of oxidative damage showed increased TBARS levels and decreased PC concentrations in victims of snakebite compared to controls (blood donors). Regarding the temporal effect, no statistical differences among the groups were detected for the distinct parameters analyzed. The responses obtained in OS biomarkers in victims of snakebite compared to healthy subjects indicate that Bothrops envenomation promoted a pronounced and persistent systemic OS in the blood of those subjects.

Keywords: Snakebites; Bothrops; Ophidic accidents; Inflammation; Oxidative stress; Antioxidants

Received: August 8, 2014; Revised: November 13, 2014; Accepted: November 19, 2014; Published: May 1, 2015  Show citation

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de Ornellas Strapazzon J, Benedetti Parisotto E, Moratelli AM, Garlet TR, Bastos J, Zimermann IR, et al.. Systemic oxidative stress in victims of Bothrops snakebites. J Appl Biomed. 2015;13(2):161-167. doi: 10.1016/j.jab.2014.11.002.
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