J Appl Biomed 14:187-197, 2016 | DOI: 10.1016/j.jab.2015.11.003
Biotherapies of rabbit serum modulate the immune response and decrease parasite load in mice infected with Trypanosoma cruzi
- a Department of Basic Health Science, Laboratory of Parasitology, Universidade Estadual de Maringá (UEM), Maringá, PR, Brazil
- b Department of Basic Health Science, Laboratory of Microbiology, Universidade Estadual de Maringá (UEM), Maringá, PR, Brazil
- c Department of Pathological Sciences, Laboratory of Experimental Immunoparasitology, Universidade Estadual de Londrina (UEL), Londrina, PR, Brazil
The use of biotherapies as intervention in murine infection with Trypanosoma cruzi is a possible means to understand the effects of these highly diluted medications. This study evaluated the effects of biotherapies that were prepared from rabbit serum uninfected (BSNI13c group) and chronically infected with Y strain of T. cruzi (BSI13c group), dynamization 13c, in mice experimentally infected. Parasitological, histopathological, and immunological parameters were evaluated. BSNI13c group exhibited the best outcome, including decreases in parasitemia and parasite load/inflammation in the heart, with pronounced Th1 response on days 8 and 12 after infection (a.i.) that was attributable to decrease in IL-4 concentrations, with no increases in TNF-α and IFN-γ, associated to decrease in IL-17A compared to control. In contrast, BSI13c group did not exhibit alterations in parasitemia but a significant decrease in parasite load/inflammation in the heart, with pronounced Th2 response on day 12 a.i. that was attributable to increase in IL-4 concentrations, with no changes in TNF-α and IFN-γ, associated to decrease in IL-17A compared to control. This study suggest that biotherapies that were prepared from rabbit serum uninfected and chronically infected with T. cruzi differentially modulate the immune system in mice infected with this protozoan, providing evidence of the actions of these medications.
Keywords: Trypanosoma cruzi; Animal model; Homeopathy; Biotherapy; Parasitemia; Tissue parasitism; Cytokines
Received: June 11, 2015; Revised: November 18, 2015; Accepted: November 19, 2015; Published: August 1, 2016 Show citation
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