Journal of Applied Biomedicine, 2016 (vol. 14), issue 3

Contents

J Appl Biomed 14:v, 2016 | DOI: 10.1016/S1214-021X(16)30139-9  

Biotherapies of rabbit serum modulate the immune response and decrease parasite load in mice infected with Trypanosoma cruzi

Fabiana Nabarro Ferraz, Franciele Karina da Veiga, Denise Lessa Aleixo, Larissa Ciupa, Benício Alves de Abreu Filho, Suelen Santos da Silva, Ivete Conchon-Costa, Wander Rogério Pavanelli, Silvana Marques de Araújo

J Appl Biomed 14:187-197, 2016 | DOI: 10.1016/j.jab.2015.11.003  

The use of biotherapies as intervention in murine infection with Trypanosoma cruzi is a possible means to understand the effects of these highly diluted medications. This study evaluated the effects of biotherapies that were prepared from rabbit serum uninfected (BSNI_13c group) and chronically infected with Y strain of T. cruzi (BSI_13c group), dynamization 13c, in mice experimentally infected. Parasitological, histopathological, and immunological parameters were evaluated. BSNI_13c group exhibited the best outcome, including decreases in parasitemia and parasite load/inflammation in the heart, with pronounced...

Phenazine-1-carboxylic acid-induced programmed cell death in human prostate cancer cells is mediated by reactive oxygen species generation and mitochondrial-related apoptotic pathway

Valliappan Karuppiah, Kumarappan Alagappan, Kannan Sivakumar, Lakshmanan Kannan

J Appl Biomed 14:199-209, 2016 | DOI: 10.1016/j.jab.2016.01.003  

Phenazine-1-carboxylic acid has extensive pharmacological activity, including antibiotic and immunomodulatory, but the anticancer activity remains unknown. Treatment of prostate cancer cell line (DU145) with phenazine-1-carboxylic acid stimulated inhibition of cell proliferation in concentration- and time-dependent manner. Dual staining confirmed phenazine-1-carboxylic acid stimulated prostate cancer programmed cell death in time-dependent manner. To investigate the exact mechanism, phenazine-1-carboxylic acid-stimulated oxidative stress and mitochondrial-related apoptotic pathway in human prostate cancer cells were examined in this study. Phenazine-1-carboxylic...

Caspase dependent apoptotic activity of Rubus fairholmianus Gard. on MCF-7 human breast cancer cell lines

Blassan P. George, Heidi Abrahamse, Thangaraj Parimelazhagan

J Appl Biomed 14:211-219, 2016 | DOI: 10.1016/j.jab.2016.02.001  

Developing plant derived chemopreventive agents that can divert the carcinogenic process and inhibit tumour progression may greatly reduce serious health consequences of cancer. This study investigated the antiproliferative and apoptosis inducing ability of Rubus fairholmianus root acetone (RFRA) on MCF-7 cells. RFRA treatments showed an increase in the in vivo antioxidant levels such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione (GSH) in mice. The extract showed significant antitumour activity with 76.57% increase in life span of ascites tumour bearing mice and 1.94 cm³ reductions...

Effect of bergenin on hepatic glucose metabolism and insulin signaling in C57BL/6J mice with high fat-diet induced type 2 diabetes

Sagadevan Ambika, Ramaligam Saravanan

J Appl Biomed 14:221-227, 2016 | DOI: 10.1016/j.jab.2016.04.002  

In this study, the therapeutic efficacy of bergenin was examined against high-fat diet (HFD) induced type 2 diabetes in C57BL/6J mice. These mice were fed continuously HFD for 16 weeks and subjected to intragastric administration of various doses (10, 20 and 40 mg/kg body weight (BW)) of bergenin daily for subsequent 8 weeks. Bergenin supplementation to HFD-fed mice lower body weight gain, plasma glucose and insulin in diabetic mice. It further restored the alterations of biochemical parameters to near normal levels in diabetic mice. As compared to other two doses of 10 mg and 20 mg, bergenin 40 mg/kg BW were showed significant protective effect on...

Antitumoral activity of novel 1,4-naphthoquinone derivative involves L-type calcium channel activation in human colorectal cancer cell line

Juan Carlos Ramos Gonçalves, Tangbadioa Herve Coulidiati, André Luís Monteiro, Laís Campos Teixeira de Carvalho-Gonçalves, Wagner de Oliveira Valença, Ronaldo Nascimento de Oliveira, Celso de Amorim Câmara, Demetrius Antônio Machado de Araújo

J Appl Biomed 14:229-234, 2016 | DOI: 10.1016/j.jab.2016.03.002  

Colorectal cancer (CRC) is an important public health problem estimated as the third most commonly diagnosed cancer worldwide. Naphthoquinones are compounds present in different families of plants and interesting for medicinal chemistry due to their activities as potent inhibitors of human cancer growth. In this way, our study aimed to evaluate the cytotoxicity and selectiveness of four 2,3-triazole-1,4-naphthoquinone derivatives (N1-N4) towards the CRC cell line HT-29 and normal human cells. MTT assay showed that N1, N2, N3 and N4 elicited distinct cytotoxic potency, exhibiting EC₅₀ values of 40.6 ± 1.0, 100.1 ± 1.0, 241.9...

Plant-mediated gold nanoparticles by Dracocephalum kotschyi as anticholinesterase agent: Synthesis, characterization, and evaluation of anticancer and antibacterial activity

Niloufar Dorosti, Fatemeh Jamshidi

J Appl Biomed 14:235-245, 2016 | DOI: 10.1016/j.jab.2016.03.001  

Biogenic metal nanoparticles owing to elimination of hazardous chemicals are beneficial for human healthy and biomedical applications. Considerable properties of these particles have motivated researchers to develop of the biosynthesized nanomaterials. In this study, the gold nanoparticles using Dracocephalum kotschyi leaf extract (d-GNPs) were synthesized and characterized by TEM-SEAD, SEM-EDAX, XRD, Zeta potential, DLS, and FT-IR analysis. TEM photograph showed spherical morphologies with an average size of 11 nm. SEAD pattern authorized fcc structure of these particles. The average zeta value of -29.3 mV revealed the surface charge of green...

Fluoride, aluminum, and aluminofluoride complexes in pathogenesis of the autism spectrum disorders: A possible role of immunoexcitotoxicity

Anna Strunecka, Russell L. Blaylock, Otakar Strunecky

J Appl Biomed 14:171-176, 2016 | DOI: 10.1016/j.jab.2016.04.001  

Autism spectrum disorders (ASD) are characterized by impairments in social interaction and communication along with stereotyped patterns of behaviors. Over the past several decades, the prevalence of ASD has increased dramatically. ASD are highly multifactorial, with many risk factors acting together. Our review suggests that most risk factors are connected to immunoexcitotoxicity. Fluoride exposure is common as a result of the artificial fluoridation of drinking water and a dramatic increase in the volume of man-made industrial fluoride compounds released into the environment. Human exposure to environmental aluminum is extensive and appears to be...

Resolving the problems and controversies surrounding temporo-mandibular mechanics

Graham Scarr, Helen Harrison

J Appl Biomed 14:177-185, 2016 | DOI: 10.1016/j.jab.2016.03.003  

The temporo-mandibular joint causes more problems than any other in the body and is the least understood with the high incidence of associated symptomatology remaining a major cause for concern. This lack of knowledge is partly due to the difficulties in acquiring information as it is not easy to access and practical and ethical constraints have ensured the almost complete absence of reliable in vivo data on joint loading and muscle forces. Whilst the issue of joint compression was debated throughout much of the twentieth-century, it is now considered that short-comings in the underlying biomechanical theory and analysis have contributed to...