J Appl Biomed 14:265-272, 2016 | DOI: 10.1016/j.jab.2016.04.004

Combination of the essential oil constituents α-pinene and β-caryophyllene as a potentiator of trypanocidal action on Trypanosoma evansi

Renata G. Amarala, Matheus D. Baldisseraa,*, Thirssa H. Grandoa, Jéssica C.M. Coutoa, Christopher P. Posserb, Andiara P. Ramosb, Michele R. Sagrillob, Rodrigo A. Vaucherb, Aleksandro S. Da Silvac, Ana P. Beckerb, Silvia G. Monteiroa,*
a Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
b Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil
c Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil

The effect of the combination of α-pinene and β-caryophyllene on the proliferation of the Trypanosoma evansi was analyzed. These compounds were added individually and in combination at different concentrations to T. evansi cultures, and the in vivo study used mice of 1.0 and 1.5 mL kg-1 of α-pinene and β-caryophyllene individually and in combination against T. evansi. In order to evaluate the in vitro safety, lymphocytes and VERO cells were assessed using tetrazolium salt (MTT) assay. The trypanocidal effect of α-pinene and β-caryophyllene was observed in vitro when applied independently or combined at 0.5, 1 and 2% concentrations. The combination effect showed a faster trypanocidal effect when compared to chemotherapy (diminazene aceturate - D.A.). Concerning the in vivo evaluation, α-pinene + β-caryophyllene at 1.0 mL kg-1 was able to extend animal longevity and showed 83.33% curative efficacy, a superior result when compared to D.A. (16.66%). The MTT assay showed that the assessed compounds do not present toxicity to used cells. Also, in vivo safety evaluation of α-pinene + β-caryophyllene at 1.0 mL kg-1 not altered the aminotransferases levels in serum. Combination between α-pinene and β-caryophyllene shows in vitro and in vivo trypanocidal activity against T. evansi, in a safe dose.

Keywords: Achyrocline satureioides; β-Caryophyllene; α-Pinene; MTT assay; "Surra"

Received: January 10, 2016; Accepted: April 27, 2016; Published: November 1, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Amaral RG, Baldissera MD, Grando TH, Couto JCM, Posser CP, Ramos AP, et al.. Combination of the essential oil constituents α-pinene and β-caryophyllene as a potentiator of trypanocidal action on Trypanosoma evansi. J Appl Biomed. 2016;14(4):265-272. doi: 10.1016/j.jab.2016.04.004.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Alaniz, F.S., Mazzarini, L.A., Demo, M.S., Sabini, L.I., Maldonado, A.M., 2010. Derived products from Achyrocline satureioides and Arnica montana, with immunomodulating effects. Mol. Med. Chem. 20, 121-124.
    2. Andrews, K.T., Fisher, G., Skinner-Adams, T.S., 2014. Drug repurposing and human parasitic protozoan diseases. Int. J. Parasitol.: Drug Drug Resist. 4, 95-111. Go to original source... Go to PubMed...
    3. Arredondo, M.F., Blasina, F., Echeverri, C., Morquio, A., Ferreira, M., Abin-Carriquiry, J.A., Lafon, L., Dajas, F., 2004. Cytoprotection by Achyrocline satureioides (Lam) D.C. and some of its main flavonoids against oxidative stress. J. Ethnopharmacol. 91, 13-20. Go to original source... Go to PubMed...
    4. Azeredo, C.M.O., Soares, M.J., 2013. Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth. Ver. Bras. Farmacogn. 23, 762-768. Go to original source...
    5. Baldissera, M.D., Da Silva, A.S., Oliveira, C.B., Zimmermann, C.E. P., Vaucher, R.A., Santos, R.C.V., Rech, V.C., Tonin, A.A., Giongo, J.L., Mattos, C.B., Koester, L., Santurio, J.M., Monteiro, S.G., 2013. Trypanocidal activity of the essential oils in their conventional and nanoemulsion forms: in vitro tests. Exp. Parasitol. 134, 356-361. Go to original source... Go to PubMed...
    6. Baldissera, M.D., Oliveira, C.B., Rech, V.C., Rezer, J.F.P., Sagrillo, M.R., Alves, M.P., Da Silva, A.P.T., Leal, D.B.R., Boligon, A.A., Athayde, M.L., Da Silva, A.S., Mendes, R.E., Monteiro, S.G., 2014. Treatment with essential oil of Achyrocline satureioides in rats infected with Trypanosoma evansi: relationship between protective effect and tissue damage. Pathol. Res. Pract. 210, 1068-1074. Go to original source... Go to PubMed...
    7. Baltz, T., Baltz, D., Giroud, C., Crockett, J., 1985. Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiensi and T. gambiense. EMBO J. 4, 1273-1277. Go to original source... Go to PubMed...
    8. Carney, J.R., Krenisky, J.M., Williamson, R.T., Luo, J., 2002. Achyrofuran, a new antihyperglycemic dibenzofuran from the South American medicinal plant Achyrocline satureioides. J. Nat. Prod. 65, 203-205. Go to original source... Go to PubMed...
    9. Colpo, C.B., Monteiro, S.G., Stainki, D.R., Colpo, E.T.B., Henriques, G.B., 2005. Natural infection by Trypanosoma evansi in dogs. Ciênc. Rural. 35, 717-719. Go to original source...
    10. Dalla Rosa, L., Da Silva, A., Gressler, L.T., Oliveira, C.B., Dambrós, M.G., Milleti, L.C., França, R.T., Lopes, S.T., Samara, Y.N., da Veiga, M.L., Monteiro, S.G., 2013. Cordycepin (30 deoxyadenosine)pentostatin(deoxycoformycin) combination treatment of mice experimentally infected with Trypanosoma evansi. Parasitology 140, 663-671. Go to original source... Go to PubMed...
    11. Da Silva, A.S., Doyle, R.L., Monteiro, S.G., 2006. Métodos de contenção e confecção de esfregaço sanguíneo para pesquisa de hemoparasitas em ratos e camundongos. Facul. Med. Vet. Zoot. Agro. 13, 83-87.
    12. Da Silva, A.S., Tochetto, C., Zanette, R.A., Pierezan, F., Rissi, D.R., Santurio, J.M., Monteiro, S.G., 2008. Aceturato de diminazeno e dipropionato de imidocarb no controle de infecção por Trypanosoma evansi em Rattus norvegicus infectados experimentalmente. Ciênc. Rural. 38, 1357-1362. Go to original source...
    13. De Souza, K.C.B., Bassani, V.L., Schapoval, E.E.S., 2007. Influence of excipients and technological process on antiinflammatory activity of quercetin and Achyrocline satureioides (Lam.) D.C. extracts by oral route. Phytomedicine 14, 102-108. Go to original source... Go to PubMed...
    14. Gantt, K.R., Goldman, T.L., McCormick, M.L., Miller, M.A., Jeronimo, S.M.B., Nascimento, E.T., Britigan, B.E., Wilson, M. E., 2001. Oxidative responses of human and murine macrophages during phagocytosis of Leishmania chagasi. J. Immunol. 167, 893-901. Go to original source... Go to PubMed...
    15. Izumi, E., Ueda-Nakamura, T., Veiga-Júnior, V.F., Pinto, A.C., Nakamura, C.V., 2012. Terpenes from Copaifera demonstrated in vitro antiparasitic and synergic activity. J. Med. Chem. 55, 2994-3001. Go to original source... Go to PubMed...
    16. Joray, M.B., Rollán, M., Del, R., Ruiz, G.M., Palacios, S.M., Carpinella, M.C., 2011. Antibacterial activity of extracts from plants of central Argentina - isolation of an active principle from Achyrocline satureioides. Planta Med. 77, 95-100. Go to original source... Go to PubMed...
    17. Kadarian, C., Broussalis, A.M., Miño, J., López, P., Gorzalczany, S., Ferraro, G., Acevedo, C., 2002. Hepatoprotective activity of Achyrocline satureioides (Lam.) DC. Pharmacol. Res. 45, 57-61. Go to original source... Go to PubMed...
    18. Kolodziej, H., Kiderlen, A.F., 2005. Antileishmanial activity and immune modulatory effects of tannins and related compounds on Leishmania parasitized RAW 264.7 cells. Phytochemistry 66, 2056-2071. Go to original source... Go to PubMed...
    19. Leal, S.M., Pino, N., Stashenko, E.E., Martínez, J.R., Escobar, P., 2013. Antiprotozoal activity of essential oils derived from Piper spp. grown in Colombia. J. Essent. Oil Res. 25, 512-519. Go to original source...
    20. Masocha, W., Rottenberg, M.E., Kristensson, K., 2007. Migration of African trypanosomes across the blood-brain barrier. Physiol. Behav. 92, 110-114. Go to original source... Go to PubMed...
    21. Mendoza-Martínez, C., Galindo-Sevilla, N., Correa-Basurto, J., Ugalde-Salvidar, V.M., Rodríguez-Delgado, R.G., HernandezPineda, J., Padierna, M., Flores-Alamo, M., Hernandez-Luis, F., 2015. Antileishmanial activity of quinazoline derivates: synthesis, docking screens, molecular dynamics simulations and electrochemical studies. Eur. J. Med. Chem. 92, 314-331. Go to original source... Go to PubMed...
    22. Molander, D.W., Wroblewski, F., Ladue, J.S., 1955. Serum glutamim oxalacetic transaminase as an index of hepatocellular integrity. J. Lab. Clin. Med. 46, 831-839.
    23. Mosman, T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63. Go to original source... Go to PubMed...
    24. Peregrine, A.S., Mamman, M., 1993. Pharmacology of diminazine: a review. Acta Trop. 54, 183-203. Go to original source... Go to PubMed...
    25. Pink, R., Hudson, A., Mouries, M.A., Bendig, M., 2005. Opportunities and challenges in antiparasitic drug discovery. Nat. Rev. Drug Discov. 4, 727-740. Go to original source... Go to PubMed...
    26. Raza, H., 2011. Dual localization of glutathiones S-transferase in the cytosol and mitochondria: implications in oxidative stress, toxicity and disease. FEBS J. 278, 4243-4251. Go to original source... Go to PubMed...
    27. Retta, D., Dellacasa, E., Villamil, J., Suárez, S.A., Bandoni, A.L., 2012. Marcela, a promising medicinal and aromatic plant from Latin America: a review. Ind. Crop. Prod. 38, 27-38. Go to original source...
    28. Rodrigues, K.A.D.F., Amorim, L.V., Dias, C.N., Moraes, D.F.C., Carneiro, S.M.P., Carvalho, F.A.D.A., 2015. Syzygium cumini (L.) Skeels essential oil and its major constituent a-pinene exhibit anti-Leishmania activity through immunomodulation in vitro. J. Ethnopharmacol. 160, 32-40. Go to original source... Go to PubMed...
    29. Rojas de Arias, A., Ferro, E., Inchausti, A., Ascurra, M., Acosta, N., Rodríguez, E., Fournet, A., 1995. Mutagenicity, insecticidal and trypanocidal activity of some Paraguayan Asteraceae. J. Ethnopharmacol. 45, 35-41. Go to original source... Go to PubMed...
    30. Sagrillo, M.R., Garcia, L.F.M., de Souza Filho, O.C., Duarte, M.M.M. F., Ribeiro, E.E., Cadoná, F.C., da Cruz, I.B.M., 2015. Tucumã fruit extracts (Astrocaryum aculeatum Meyer) decrease cytotoxic effects of hydrogen peroxide on human lymphocytes. Food Chem. 173, 741-748. Go to original source... Go to PubMed...
    31. Spinosa, H.S., Górniak, S.L., Bernardi, M.M., 1999. Farmacologia aplicada a medicina veterinária. Guanabara Koogan, Rio de Janeiro692.
    32. Vogt, V., Tonn, C., Sabini, L., Rosas, S., 2010. Fungitoxic effects of Achyrocline satureioides (marcela) on plant pathogens. Mol. Med. Chem. 21, 109-112.
    33. Wilms, L.C., Hollman, P.C.H., Boots, A.W., Kleinjans, J.C.S., 2005. Protection by quercetin and quercetin-rich fruit juice against induction of oxidative DNA damage and formation of BPDE- DNA adducts in human lymphocytes. Mutat. Res. 582, 155- 162. Go to original source... Go to PubMed...
    34. Wuyts, N., Chokesajjawatee, N., Sarataphan, N., Panyim, S., 1995. PCR amplification of crude blood on microscope slides in the diagnosis of Trypanosoma evansi infection in dairy cattle. Ann. Soc. Bel. 75, 229-237.

    Return to the content