J Appl Biomed 15:39-47, 2017 | DOI: 10.1016/j.jab.2016.09.007

Zinc supplementation modifies trace element status in exercised rats

Andrey A. Skalny^a,b, Yulia S. Medvedeva^c, Irina B. Alchinova^c, Eugenia R. Gatiatulina^d,e, Ivan V. Radysh^b, Mikhail Yu. Karganov^c, Anatoly V. Skalny^b,f,g,h, Alexandr A. Nikonorov^d,h, Alexey A. Tinkov^b,d,f,h,*

^a Ryazan State Medical University named after academician IP Pavlov, Visokovoltnaya St. 9, Ryazan 390026, Russia

^b RUDN University, Miklukho-Maklai St. 6, Moscow 117198, Russia

^c Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Baltiyskaya St. 8, Moscow 125315, Russia

^d Department of Biochemistry, Orenburg State Medical University, Sovetskaya St. 6, Orenburg 460000, Russia

^e Department of Biochemistry, South Ural State Medical University, Vorovskogo St. 64, Chelyabinsk, 454048, Russia

^f Laboratory of Biotechnology and Applied Bioelementology, Yaroslavl State University, Sovetskaya St. 14, Yaroslavl, 150000, Russia

^g All-Russian Research Institute of Medicinal and Aromatic Plants (VILAR), Grina St. 7, Moscow, 117216, Russia

^h Institute of Bioelementology (Russian Satellite Centre of Trace Element-Institute for UNESCO), Orenburg State University, Pobedy Ave. 13, Orenburg 460352, Russia

Purpose
Investigation of the effect of exercise and zinc (Zn) supplementation on trace element status in rats.
Methods
24 male Wistar were divided into four groups: control, exercised, Zn-supplemented (15 mg/kg weight Zn asparaginate), exercised Zn-supplemented. Zn was supplemented as Zn asparaginate. Serum lactate and creatinine levels, and creatine kinase activity were assessed. Tissue trace elements were estimated using inductively coupled plasma mass spectrometry.
Results
Exercise significantly increased lactate, and creatinine levels. Exercise significantly decreased muscle, kidney, and hair Zn; liver, muscle and serum Co; serum Fe; myocardial and hair Cu; liver, heart, skeletal muscle and kidney Se levels. Oppositely, exercise results in elevation of liver Zn; heart and skeletal muscle, kidney and hair Fe; kidney Cu; liver and hair Mn; serum and hair Se content. Zn supplementation reduced exercise-induced increase in lactate and creatinine levels, and elevated liver, kidney, heart, and hair Zn content in exercised rats. Supplementation with Zn reversed exercise-induced decrease in Co levels and increased Fe and Se stores in animals with high physical activity.
Conclusions
Beneficial effect of zinc supplementation in exercised organism may be associated not only with modulation of zinc status but regulation of other essential trace elements status and their biological effects.

Keywords: Zinc; Cobalt; Iron; Selenium; Running; Physical activity

Received: May 16, 2016; Accepted: September 29, 2016; Published: January 1, 2017  Show citation