J Appl Biomed 15:187-195, 2017 | DOI: 10.1016/j.jab.2017.01.002

Monoterpene alpha-terpinene induced hepatic oxidative, cytotoxic and genotoxic damage is associated to caspase activation in rats

Matheus D. Baldisseraa,*, Carine F. Souzab, Geisa S. Dolcib, Thirssa H. Grandoa, Michele R. Sagrilloc, Rodrigo A. Vaucherc, Sônia C.A. da Luza, Sérgio O. Silveirad, Marta M.M.F. Duarted, Thiago Duarted, Aleksandro S. da Silvae, Silvia G. Monteiroa,*
a Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
b Department of Physiology and Pharmacology, UFSM, Santa Maria, RS, Brazil
c Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil
d Department of Biochemistry, UFSM, Santa Maria, RS, Brazil
e Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil

The aim of this study was to investigate the occurrence of toxic effects in liver tissue of rats treated with α-terpinene. All treatments were intraperitoneally administered at doses of 0.5, 0.75 and 1.0 ml kg-1 during 10 days. Liver samples were collected and assessed by histopathological analysis, caspases -1, -3, -8 assay, biomarkers of hepatic damage and determination of oxidant/antioxidant status (thiobarbituric acid-reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione S-transferase (GST) and glutathione peroxidase (GPx)). Additionally, the cytotoxic and genotoxic effects were evaluated by comet assay. An increase was observed on TBARS levels and GPx activity on the hepatic tissue. Instead, CAT and SOD activities decreased in rats treated with a dose of 1.0 ml kg-1 of α-terpinene. Concomitantly, ROS levels increased and GST levels decreased in rats treated with α-terpinene at doses of 0.5, 0.75 and 1.0 ml kg-1. Also, there was an increase in frequency of damage, damage index and caspases, while cell viability decreased in rats treated with α-terpinene. Alanine aminotransferase and aspartate aminotransferase increased in rats treated with 1.0 ml kg-1 of α-terpinene. Therefore, α-terpinene induces oxidative stress, cytotoxic and genotoxic effects in liver tissue involving the caspases activation.

Keywords: Apoptosis; Cell viability; DNA; Monoterpenes

Received: December 15, 2016; Accepted: January 12, 2017; Published: July 1, 2017  Show citation

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Baldissera MD, Souza CF, Dolci GS, Grando TH, Sagrillo MR, Vaucher RA, et al.. Monoterpene alpha-terpinene induced hepatic oxidative, cytotoxic and genotoxic damage is associated to caspase activation in rats. J Appl Biomed. 2017;15(3):187-195. doi: 10.1016/j.jab.2017.01.002.
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