Journal of Applied Biomedicine, 2017 (vol. 15), issue 3

Contents

J Appl Biomed 15:v, 2017 | DOI: 10.1016/S1214-021X(17)30231-4  

Maturation rates of oocytes and levels of FSHR, LHR and GnRHR of COCs response to FSH concentrations in IVM media for sheep

Wei Suocheng, Gong Zhuandi, Sheng Li, Liang Haoqin, Lai Luju, Deng Yingying

J Appl Biomed 15:180-186, 2017 | DOI: 10.1016/j.jab.2017.01.001  

This study investigated the FSH influence on maturation rates of oocytes in vitro maturation (IVM), and expression levels of the follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR) and gonadotropin releasing hormone receptor (GnRHR) of cumulus-oocyte complexes (COCs) response to FSH treatment. 1686 COCs were harvested from 1063 ovaries of sheep. COCs were cultured 26 h at 38.5 °C and 5.0% CO₂ in IVM media supplemented with 0, 5, 10, 20 and 30 IU/ml FSH. They were allocated in to FSH-1 (basal line), FSH-2, FSH-3, FSH-4 and FSH-5 groups. The apoptosis of COCs was assessed by Tunel assay....

Monoterpene alpha-terpinene induced hepatic oxidative, cytotoxic and genotoxic damage is associated to caspase activation in rats

Matheus D. Baldissera, Carine F. Souza, Geisa S. Dolci, Thirssa H. Grando, Michele R. Sagrillo, Rodrigo A. Vaucher, Sônia C.A. da Luz, Sérgio O. Silveira, Marta M.M.F. Duarte, Thiago Duarte, Aleksandro S. da Silva, Silvia G. Monteiro

J Appl Biomed 15:187-195, 2017 | DOI: 10.1016/j.jab.2017.01.002  

The aim of this study was to investigate the occurrence of toxic effects in liver tissue of rats treated with α-terpinene. All treatments were intraperitoneally administered at doses of 0.5, 0.75 and 1.0 ml kg^-1 during 10 days. Liver samples were collected and assessed by histopathological analysis, caspases -1, -3, -8 assay, biomarkers of hepatic damage and determination of oxidant/antioxidant status (thiobarbituric acid-reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione S-transferase (GST) and glutathione peroxidase (GPx)). Additionally, the cytotoxic and genotoxic...

Alpha 2,3- and alpha 2,6-sialylation of human skim milk glycoproteins during milk maturation

Jolanta Lis-Kuberka, Marta Berghausen-Mazur, Magdalena Orczyk-Pawiłowicz

J Appl Biomed 15:196-203, 2017 | DOI: 10.1016/j.jab.2017.02.001  

Human milk is a source of glycoconjugates, sialylated forms of which enrich the newborn immature immune system and are crucial for their proper development and well-being. Here, we analyzed the expression of α2,3-/α2,6-sialylated glycotopes on skim milk glycoproteins over lactation. Milk samples were analyzed by lectin-blotting using α2,3- and α2,6- sialic acid specific Maackia amurensis (MAA) and Sambucus nigra (SNA) lectins and sialyl- and asialyl-T antigen specific Artocarpus integrifolia (Jacalin) and Arachis hypogaea (PNA) lectins. The reactivities of MAA, SNA, Jacalin and PNA with milk glycoproteins...

Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function

Dragana Totoskovic, Violeta Dopsaj, Jelena Martinovic

J Appl Biomed 15:204-209, 2017 | DOI: 10.1016/j.jab.2017.02.002  

The purpose of the present study was to investigate neutrophil and monocyte cell population data as novel markers of low cobalamin/folate concentrations and influence of renal function on their usefulness. The study included 284 patients older than 60 years or with dyspepsia symptoms with mean corpuscular volume 80-100 fL and C-reactive protein ≤ 50 mg/l. Subjects were divided according to renal function and further classified based on cobalamin and folate levels. Neutrophil and monocyte volume (NeV, MoV), conductivity (NeC, MoC), light scatter (NeS, MoS) and standard deviations (NeV-SD, MoV-SD, NeC-SD, MoC-SD, NeS-SD, MoS-SD), obtained by Coulter...

β-Cyclodextrin modified mesoporous silica nanoparticles as a nano-carrier: Response surface methodology to investigate and optimize loading and release processes for curcumin delivery

Behnaz Abdous, S. Maryam Sajjadi, Leila Ma'mani

J Appl Biomed 15:210-218, 2017 | DOI: 10.1016/j.jab.2017.02.004  

β-Cyclodextrin functionalized PEGylated porous silica nanoparticles KIT-6 (denoted as [β-CD@PEGylated KIT-6] NPs) is synthesized and evaluated as an efficient and reliable pH-sensitive nano-carrier. Curcumin (CUR), an anticancer drug, has low solubility and stability and these properties diminished its bioavailability. One way to overcome this problem is employing nano-carrier for delivery of CUR. In this study, the novel [β-CD@PEGylated KIT-6] NPs nano-carrier was employed for CUR delivery successfully. The nano-DDS was characterized using different techniques such as X-ray powder diffraction (XRD), transmission and scanning electron...

Anticancer effect and enhancement of therapeutic potential of Vincristine by extract from aerial parts of Juniperus excelsa on pre-B acute lymphoblastic leukemia cell lines

Mina Darvishi, Somayeh Esmaeili, Nasrin Dehghan-Nayeri, Pargol Mashati, Ahmad Gharehbaghian

J Appl Biomed 15:219-226, 2017 | DOI: 10.1016/j.jab.2017.04.002  

The natural products and conventional chemotherapeutic drugs combination can increase the efficacy of anticancer treatment through their potential synergistic effects and reduce its toxicity. The current study investigates the effect of methanolic extract from aerial parts of Juniperus excelsa on cell death activities induced by vincristine in acute lymphoblastic leukemia cells.Cytotoxic activity of J. excelsa extract and vincristine in Nalm-6 and Reh cells was determined using MTT assay and synergism was evaluated using the CompuSyn software. Apoptosis was assessed by caspase 3 activity assay and flow cytometry. The expression levels...

Impact of hormonal changes on the semen quality and assisted reproductive outcomes in infertile men

Fatemeh Ghasemian, Seyed Abolghasem Mirroshandel, Ziba Zahiri

J Appl Biomed 15:227-232, 2017 | DOI: 10.1016/j.jab.2017.04.003  

This study investigated the effect of hormonal changes on semen quality, chromatin status, and assisted reproductive outcomes (intracytoplasmic sperm injection), among infertile men. In this research, 219 infertile men undergoing assisted reproductive treatment were evaluated with reproductive hormone levels (including follicle-stimulating hormone, luteinizing hormone and testosterone), semen parameters, and sperm chromatin integrity and condensation, between 2012 and 2014. Finally, the assisted reproductive outcomes in these infertile men were studied. The low rate of total sperm count, motility and morphology, fertilization and the high percentage...

Design and drug-like properties of new 5-methoxysalicylaldehyde based hydrazones with anti-breast cancer activity

Boryana Nikolova-Mladenova, Georgi Momekov, Darvin Ivanov, Adriana Bakalova

J Appl Biomed 15:233-240, 2017 | DOI: 10.1016/j.jab.2017.04.004  

Novel benzoylhydrazones were designed and synthesized by condensation of 5-methoxysalicylaldehyde and benzhydrazides with different substituents at 4th position. The structures of the new derivatives were confirmed by elemental and thermal analysis, mass, IR, ¹H NMR and ¹³C NMR spectroscopy. The molecular properties of the compounds, important for drug pharmacokinetics and biodisposition in the human body, were assessed by the Lipinski's rule of five. In silico evaluation of the LogP value and the remaining parameters of drug similarity, as well as the topological polar surface area and absorption percentage, were used...

Hits and defeats of genome-wide association studies of atopy and asthma

Hanna Danielewicz

J Appl Biomed 15:161-168, 2017 | DOI: 10.1016/j.jab.2017.03.002  

Atopy and asthma are complex conditions, recognised as outcomes in which both genes and environment play crucial role. Large number of disease associated loci have been identified within GWAS approach over last years and the knowledge of pathobiology of asthma and allergy has widened substantially. However still the results achieved are difficult to interpret. Most markers have no clear function on and expound small portion of heritability. The "missed heritability" could be hidden in the gene-by environment interactions. The most know environmental factor which interacts with asthma and atopy is farming. The common link between genes and environment...

The role of keratinocytes in inflammation

Jana Juráňová, Jana Franková, Jitka Ulrichová

J Appl Biomed 15:169-179, 2017 | DOI: 10.1016/j.jab.2017.05.003  

The epidermis is the external layer of the skin and is composed mainly of keratinocytes. Therefore, keratinocytes play an indispensable role as inherent constituents of the skin barrier in physical defenses against environmental threats. Keratinocytes also exert an active protective role against invasion by pathogens. This competency is of particular importance when physical defenses fail as a consequence of skin injury. During the inflammatory phase of healing, keratinocytes act as immuno-modulators, managing inflammation via a rigorously coordinated network of inflammatory cascades, triggered by keratinocyte-receptor communication with the surroundings...