J Appl Biomed 16:100-110, 2018 | DOI: 10.1016/j.jab.2017.11.001

Antioxidative effects of aqueous extract of broccoli sprouts against Triazophos induced hepatic and renal toxicity in female Wistar rats

Dharmender Sharma, Gurinder Kaur Sangha^*

Punjab Agricultural University, College of Basic Sciences and Humanities, Department of Zoology, Ludhiana, India

Oxidative stress (OS) is a major cause of hepatic and renal disorders, so present investigation was designed to evaluate the antioxidative efficacy of aqueous broccoli extract (BE) via three different doses - 10, 20 and 30 mmol - of glucosinolates against toxic effects of triazophos (TZ), an organophosphorous pesticide, in female rats during 30 days experiment. Six groups of rats were made and were orally intubated with TZ and BE as per experimental design. TZ and BE induced OS biomarkers of hepatic and renal toxicity - ALT, AST, urea and creatinine - were noticed in plasma, while catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), lipid peroxidation (LPO) were estimated in liver and kidney along with histological and apoptotic observation. Plasma ALT, AST, urea and creatinine levels along with organ OS parameters as CAT, SOD, GST and GPx were subtly improved in all BE + TZ treated rats. Decreased LPO and reduced apoptosis along with improved histoarchitecture was observed in all BE + TZ treated rats. Present study suggest that the administration of broccoli extract and TZ combination in rats can prevent severe alterations of hepatic and renal biochemical markers and disruptions of histological structure by antioxidative potential of BE from sprouts.

Keywords: Antioxidant enzymes; Extract; Liver; Kidney; Oxidative stress; Triazophos

Received: April 19, 2017; Revised: August 21, 2017; Accepted: November 2, 2017; Published: May 1, 2018  Show citation