Journal of Applied Biomedicine, 2018 (vol. 16), issue 2

Contents

J Appl Biomed 16:v, 2018 | DOI: 10.1016/S1214-021X(18)30086-3  

Antioxidative effects of aqueous extract of broccoli sprouts against Triazophos induced hepatic and renal toxicity in female Wistar rats

Dharmender Sharma, Gurinder Kaur Sangha

J Appl Biomed 16:100-110, 2018 | DOI: 10.1016/j.jab.2017.11.001  

Oxidative stress (OS) is a major cause of hepatic and renal disorders, so present investigation was designed to evaluate the antioxidative efficacy of aqueous broccoli extract (BE) via three different doses - 10, 20 and 30 mmol - of glucosinolates against toxic effects of triazophos (TZ), an organophosphorous pesticide, in female rats during 30 days experiment. Six groups of rats were made and were orally intubated with TZ and BE as per experimental design. TZ and BE induced OS biomarkers of hepatic and renal toxicity - ALT, AST, urea and creatinine - were noticed in plasma, while catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx),...

Effect of pramipexole alginate nanodispersion (PAND) on the transgenic Drosophila expressing human alpha synuclein in the brain

Yasir Hasan Siddique, Falaq Naz, Wasi Khan, Smita Jyoti, Braj Raj Singh, Alim Hussain Naqvi

J Appl Biomed 16:111-119, 2018 | DOI: 10.1016/j.jab.2017.11.002  

In the present study the effect of pramipexole alginate nanodispersion (PAND) was studied on the transgenic Drosophila exhibiting the PD symptoms. The PD flies were allowed to feed on the diet having PAND at final concentration of 1, 2 and 3 μM. A dose dependent significant delay in the loss of climbing ability and improvement in the activity was observed in PD flies. A dose dependent significant change in the oxidative stress markers and dopamine content was also observed in the PD flies exposed to various doses PAND. The improvement in the PD symptoms was more in PD flies exposed to PAND compared to PD flies exposed to pramipexole alone.

Antibacterial activity of green silver nanoparticles synthesized from Anogeissus acuminata against multidrug resistant urinary tract infecting bacteria in vitro and host-toxicity testing

Monali P. Mishra, Rabindra N. Padhy

J Appl Biomed 16:120-125, 2018 | DOI: 10.1016/j.jab.2017.11.003  

Silver nanoparticles (AgNPs) with aqueous leaf-extract of the timber-yielding plant Anogeissus acuminata were synthesized for in vitro control of pathogenic bacteria. Characterization of AgNPs with ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, X-Ray diffraction (XRD) study and Fourier transformed infrared spectroscopy (FTIR) study was done for a confirmation of the synthesis. The SEM analysis confirmed that the metal particles were below 100 nm size. The antibacterial activity of AgNPs was monitored by agar-well diffusion method against 11 multidrug resistant (MDR) urinary...

Inotodiol protects PC12 cells against injury induced by oxygen and glucose deprivation/restoration through inhibiting oxidative stress and apoptosis

Yan Li, Wenting Zhang, Chun Chen, Chunping Zhang, Jingyu Duan, Huankai Yao, Qunli Wei, Aiguo Meng, Jun Shi

J Appl Biomed 16:126-132, 2018 | DOI: 10.1016/j.jab.2017.11.004  

Ischemic stroke is a severe cause of disability and death all over the world. To search for effective therapy for ischemic stroke, PC12 cells damaged by oxygenation and glucose deprivation/restoration were employed to assess the protective effects of inotodiol. As a result, inotodiol can improve the cell viability and attenuate the leakage of lactate dehydrogenase. Meanwhile, inotodiol can prevent oxidative stress by reducing reactive oxygen species generation, decreasing the content of malonic dialdehyde, and increasing the activity of superoxide dismutase. In addition, the dysfunction of mitochondria induced by oxygenation and glucose deprivation/restoration...

Caveolin-1 rs4730751 gene polymorphism in kidney allograft recipients

Sylwia Słuczanowska-Głąbowska, Damian Malinowski, Krzysztof Safranow, Leszek Domański, Michał Czerewaty, Przemysław Ustianowski, Maria Laszczyńska, Andrzej Pawlik

J Appl Biomed 16:133-137, 2018 | DOI: 10.1016/j.jab.2017.11.007  

Caveolin-1 is a phosphoprotein that plays a crucial role in tissue remodeling and fibrotic pathways, controlling fibroblast function, proliferation, and apoptosis. Here we aimed to examine the association between the CAV1 rs4730751 gene polymorphism and kidney graft function, graft histology, and graft survival. This study enrolled 270 Caucasian deceased - donor renal transplant recipients. We found no statistically significant associations between CAV1 rs4730751 and delayed graft function, acute rejection, or chronic allograft dysfunction, as well as creatinine values at 1-60 months after transplantation, the risk of graft loss, dialysis...

In vivo assessment of time dependent changes of T2* in medial meniscus under loading at 3T: A preliminary study

Lenka Hornakova, Vladimir Juras, Petr Kubovy, Daniel Hadraba, David Gerych, Pavel Stursa, Xeni Deligianni, Oliver Bieri, Siegfried Trattnig, Karel Jelen

J Appl Biomed 16:138-144, 2018 | DOI: 10.1016/j.jab.2017.12.001  

Due to the internal structure of the knee joint, the ability to characterize and quantify the dynamic response of the meniscal tissue directly in vivo is highly problematic. The main purpose of this study was to investigate the behaviour of the meniscus under loading conditions. Four healthy young females were included. To obtain T2* values in the meniscus, the vTE sequence was used with 10 echoes ranging from 0.8 to 10.1 ms. Submilisecond first echo time is a great advantage of vTE sequence allowing for precise mapping of relatively short T2*. The two-parametric least squares fitting procedure was used to calculate T2* pixel-wise. A custom-made...

Nonlinear Heart Rate Variability based artificial intelligence in lung cancer prediction

Reema Shyamsunder Shukla, Yogender Aggarwal

J Appl Biomed 16:145-155, 2018 | DOI: 10.1016/j.jab.2017.12.002  

Lung cancer is uncontrolled growth of cells that occurs due to exposure to smoke, radiation and chemicals, which causes chronic stress and associated with impaired autonomic nervous system. Nonlinear heart rate variability (HRV) analysis has been suggested to uncover the performance status of lung cancer subjects and distinguish them from healthy controls. The present work obtained tachogram from recorded electrocardiogram of 104 lung cancer subjects and 30 healthy controls to extract HRV indices. The obtained results suggested lowered HRV (altered autonomic nervous system tone) values from Eastern Cooperative Oncology Group (ECOG) 1 to ECOG4. Subject...

Propolis: The future therapy against Helicobacter pylori-mediated gastrointestinal diseases

Ummay Mahfuza Shapla, Jinat Raihan, Asiful Islam, Fahmida Alam, Naeem Solayman, Siew Hua Gan, Sakib Hossen, Ibrahim Khalil

J Appl Biomed 16:81-99, 2018 | DOI: 10.1016/j.jab.2017.10.007  

Helicobacter pylori (H. pylori), which is found in the stomach of approximately 50% of humans, remains there for almost the entire lifetime of the infected individual, leading to various gastrointestinal tract-associated disorders following full-blown infection. Due to the emergence of antibiotic resistance, recurrence and high cost of therapy, most antibiotic-based treatment strategies are not very effective in eradicating H. pylori infections. The quest for an alternative treatment free of these inconveniences is currently in demand. One of the important alternatives is propolis, produced by the honeybee Apis mellifera, which...