J Appl Biomed 16:120-125, 2018 | DOI: 10.1016/j.jab.2017.11.003

Antibacterial activity of green silver nanoparticles synthesized from Anogeissus acuminata against multidrug resistant urinary tract infecting bacteria in vitro and host-toxicity testing

Monali P. Mishra, Rabindra N. Padhy^*

Siksha 'O' Anusandhan University, IMS and Sum Hospital, Central Research Laboratory, Kalinga Nagar, Bhubaneswar, Odisha, India

Silver nanoparticles (AgNPs) with aqueous leaf-extract of the timber-yielding plant Anogeissus acuminata were synthesized for in vitro control of pathogenic bacteria. Characterization of AgNPs with ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, X-Ray diffraction (XRD) study and Fourier transformed infrared spectroscopy (FTIR) study was done for a confirmation of the synthesis. The SEM analysis confirmed that the metal particles were below 100 nm size. The antibacterial activity of AgNPs was monitored by agar-well diffusion method against 11 multidrug resistant (MDR) urinary tract infection (UTI) causing pathogenic bacteria, isolated from clinical samples. At 15 μg/ml AgNPs, values of the zone of inhibition (ZI) ranged from 19 to 13 mm, while against the standard antibiotic, gentamicin 30 μg/ml ZI ranged from 28 to 20 mm. Host toxicity testing of AgNPs with cultured lymphocytes from human umbilical cord blood in vitro was done; at 3000 mg/l AgNPs, 25% of cell death occurred. Thus, the synthesized AgNPs with aqueous leaf extract of A. acuminata could control most MDR UTI bacteria without any toxicity to human lymphocytes.

Keywords: Silver nanoparticles; Anogeissus acuminate; Multidrug resistant bacteria; Urinary tract infection; Scanning electron microscopy; X-Ray diffraction

Received: January 14, 2017; Revised: October 6, 2017; Accepted: November 23, 2017; Published: May 1, 2018  Show citation