J Appl Biomed 16:198-207, 2018 | DOI: 10.1016/j.jab.2018.01.008

Induction of caspase-mediated apoptosis using Alnus japonica extracts in AGS human gastric carcinoma cells

Seong-Eun Kima, Yon-Suk Kima,c, Woen-Bin Shina,b, Jin-Su Parka,b, Sang-Ho Moonc,d, Byong-Tae Jeone, Pyo-Jam Parka,b,c,*
a Konkuk University, Department of Biotechnology, Chungju, South Korea
b Konkuk University, Department of Applied Life Science, Chungju, South Korea
c Konkuk University, Medicinal Biological Resources Research Institute, Chungju, South Korea
d Konkuk University, Division of Food Bio Science, Chungju, South Korea
e Hasung Corp., Seoul, South Korea

Alnus japonica has been used as a traditional oriental medicine for many diseases such as fever, haemorrhage and alcoholism. In this study, A. japonica extracts were evaluated for their in vitro antioxidant potentials and anticancer effects in AGS human gastric carcinoma cell line. The antioxidant properties of A. japonica extracts were evaluated using several biochemical assays, including FRAP (ferric reducing antioxidant power) assay, ABTS (2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), alkyl and hydroxyl radical scavenging activity assay. Our study showed that ethanol extract of A. japonica (AJE) has a more potent antioxidant activity than its water extract. In addition, AJE extract inhibited the cell growth and induced the cell death by increasing reactive oxygen species (ROS) production in AGS cells. Moreover, AJE extract specifically triggered the apoptosis mediated through the activation of caspase-8, 7, 3, and poly-ADP ribose polymerase (PARP). Thus, these results suggest that AJE extract could be potentially useful as a new promising strategy in the therapy for gastric carcinoma cancer.

Keywords: Alnus japonica; Antioxidant; Apoptosis; AGS human gastric carcinoma cells; Caspase-dependent pathway

Received: August 24, 2016; Revised: April 21, 2017; Accepted: January 31, 2018; Published: August 1, 2018  Show citation

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Seong-Eun K, Yon-Suk K, Shin W, Park J, Moon S, Jeon B, Park P. Induction of caspase-mediated apoptosis using Alnus japonica extracts in AGS human gastric carcinoma cells. J Appl Biomed. 2018;16(3):198-207. doi: 10.1016/j.jab.2018.01.008.
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