J Appl Biomed 16:208-213, 2018 | DOI: 10.1016/j.jab.2017.12.004

NTPDase, 5'-nucleotidase and adenosine deaminase activities and purine levels in serum of sickle cell anemia patients

Lívia G. Castilhosa, Stephen A. Adefeghab,c, Pedro H. Doleskib, Tatiana M.D. Bertoldoa, Cesar Eduardo J. Moritzd, Emerson A. Casalie, Daniela B.R. Leala,b,*
a Federal University of Santa Maria, Post-Graduate Program in Pharmaceutical Sciences, Santa Maria, RS, Brazil
b Federal University of Santa Maria, Post-Graduate Program in Toxicological Biochemistry, Santa Maria, RS, Brazil
c Federal University of Technology, Department of Biochemistry, Akure, Nigeria
d Federal University of Rio Grande do Sul, Post-Graduate Program in Science of Human Movement, Porto Alegre, RS, Brazil
e Federal University of Rio Grande do sul, Departament of Morphological Sciences, Porto Alegre, RS, Brazil

Sickle cell anemia (SCA) is a hereditary disorder that is characterized by tendency of hemoglobin molecules within the erythrocytes to polymerize under hypoxia conditions, deform the red cells, and promote vaso-occlusion and endothelial damage. This disease may be associated with the extracellular release of nucleotides, particularly ATP, ADP and adenosine into the circulation. The aim of this study was to investigate the possible changes in adenine nucleotides and nucleoside metabolizing enzymes as well as their levels in serum of SCA patients. NTPDase, 5'-nucleotidase and adenosine deaminase activities were evaluated in serum obtained from blood samples of 15 SCA treated patients and 15 healthy subjects (control group). The results revealed that there were significant (P < 0.001) elevations in NTPDase, 5'-nucleotidase and ADA activities in serum of SCA treated patients when compared to the control group. Furthermore, no significant (P > 0.05) alteration was observed in ATP, ADP and adenosine levels of both SCA treated and control groups. However, inosine level was significantly (P < 0.05) decreased and hypoxanthine level was higher in SCA treated patients (P < 0.001) when compared to the control group. The results suggest the involvement of purinergic signaling enzymes in the maintenance of the levels of extracellular nucleotides and nucleosides, thus preventing some pathophysiological conditions associated with SCA.

Keywords: NTPDase; 5'-nucleotidase; Adenosine deaminase; Serum; Sickle cell anemia

Received: May 31, 2016; Revised: November 25, 2017; Accepted: December 12, 2017; Published: August 1, 2018  Show citation

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Castilhos LG, Adefegha SA, Doleski PH, Bertoldo TMD, Moritz CEJ, Casali EA, Leal DBR. NTPDase, 5'-nucleotidase and adenosine deaminase activities and purine levels in serum of sickle cell anemia patients. J Appl Biomed. 2018;16(3):208-213. doi: 10.1016/j.jab.2017.12.004.
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