J Appl Biomed 16:221-231, 2018 | DOI: 10.1016/j.jab.2018.02.002

Fruit waste (peel) as bio-reductant to synthesize silver nanoparticles with antimicrobial, antioxidant and cytotoxic activities

Annua, Shakeel Ahmeda,d, Gurpreet Kaurb,c, Praveen Sharmac, Sandeep Singhc, Saiqa Ikrama,*
a Jamia Millia Islamia, Department of Chemistry, Bio/Polymers Research Laboratory, New Delhi, India
b Central University of Punjab, Centre for Environmental Science and Technology, Bathinda, India
c Central University of Punjab, Centre for Human Genetics and Molecular Medicine, Laboratory of Molecular Medicine, Bathinda, India
d Department of Chemistry, Government Degree College Mendhar, Jammu and Kashmir, India

Since last decade, biogenic synthesis of metal or metal-oxide nanoparticles is emerging as an alternative method, which is environment friendly, simple and safe to use. In this article, fruit waste (peel) extract (FWE) of three citrus fruits viz. Citrus limon, Citrus sinensis, and Citrus limetta were used as bio-reductant for green and sustainable synthesis of silver nanoparticles (AgNPs). As-synthesised AgNPs were characterized by using UV-vis spectroscopy, Dynamic light scattering, and High Resolution Transmission Electron Microscopy. TEM studies revealed 9-46 nm size range of synthesized AgNPs. The antimicrobial and antioxidant activities were also studied by using Agar well diffusion method and DPPH Assay, respectively. Nanoparticles showed good antimicrobial activity against both Gram positive (S. aureus) and Gram negative (E. coli) bacteria. Further, bioactivity assays revealed selective cytotoxicity (anticancer) of the nanoparticles against human lung cancer cell line A549. The nanoparticles are able to induce cancer cell specific apoptosis at G0/G1 phase of cell cycle. The results showed potential mechanism of action of nanoparticles via augmentation of antioxidant system in cancer cells. Over all, this study show multifaceted potential bioactivities of nanoparticles generated from fruit waste.

Keywords: Biosynthesis; Citrus fruits; Silver nanoparticles; Antimicrobial; Antioxidant; Anticancer

Received: March 21, 2017; Revised: December 8, 2017; Accepted: February 5, 2018; Published: August 1, 2018  Show citation

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Annu, Ahmed S, Kaur G, Sharma P, Singh S, Ikram S. Fruit waste (peel) as bio-reductant to synthesize silver nanoparticles with antimicrobial, antioxidant and cytotoxic activities. J Appl Biomed. 2018;16(3):221-231. doi: 10.1016/j.jab.2018.02.002.
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