J Appl Biomed 16:301-310, 2018 | DOI: 10.1016/j.jab.2018.06.001

Berbamine protects the heart from isoproterenol induced myocardial infarction by modulating eNOS and iNOS expressions in rats

Sithuraj Saranyaa, ViswanadhaVijaya Padmaa,b,c,*
a Bharathiar University, Department of Biotechnology, Translational Research Laboratory, Tamil Nadu, India
b China Medical University, Graduate Institute of Basic Medical Science, Taichung, Taiwan
c Asia University, Department of Health and Nutrition Biotechnology, Taichung, Taiwan

Aim
The current study was designed to investigate the effect of berbamine (BBM) on isoproterenol (ISO) induced changes in cardiac marker enzymes, myocardial oxidative stress, lipid profile and expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in male Wistar rats. Rats were pretreated with BBM (25 mg/kg) through intraperitoneal injection for 7 days followed by induction of myocardial infarction (MI) by subcutaneous injection of ISO (85 mg/kg) for last two days. Key findings: In the present study, the histopathological findings of the heart tissue showed that BBM treatment significantly minimized the damage induced by ISO. BBM pretreatment showed a significant decrease in heart weight, serum marker enzymes, lipid peroxidation and significant increase in cardiac endogenous enzymatic and non-enzymatic antioxidants compared to the ISO-treated group. In addition, we observed significantly upregulated eNOS expression and downregulated iNOS expression in BBM pretreated group. Thus, BBM protected the rat's heart from ISO-induced myocardial infarction by its antioxidant, and antilipidemic properties. Significance: The results of the present investigation suggested that BBM efficiently ameliorated the ISO-induced myocardial infarction in rats.

Keywords: Myocardial infarction; Isoproterenol; Berbamine; Antioxidant; iNOS; eNOS

Received: June 19, 2017; Revised: May 16, 2018; Accepted: June 4, 2018; Published: November 1, 2018  Show citation

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Sithuraj S, Viswanadha VP. Berbamine protects the heart from isoproterenol induced myocardial infarction by modulating eNOS and iNOS expressions in rats. J Appl Biomed. 2018;16(4):301-310. doi: 10.1016/j.jab.2018.06.001.
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