J Appl Biomed 16:358-361, 2018 | DOI: 10.1016/j.jab.2018.01.009

RIG-1 and MDA5 are the important intracellular sensors against bacteria in septicemia suffering patients

Asieh Asadpour-Behzadi, Ashraf Kariminik^*

Department of Microbiology,Kerman Branch, Islamic Azad University, Kerman, Iran

Background
MDA5 and RIG-1 are the important intracellular receptors which detect microbial associated molecular patterns. Septicemia is a condition in which infection enters the bloodstream of the patients. The main intracellular mechanisms against septicemia are yet to be clarified. Therefore, this research study was aimed to evaluate expression of MDA5 and RIG-1 in the patients suffering from septicemia in comparison to healthy controls.
Methods
MDA5 and RIG-1 expression levels in 40 patients suffering from septicemia and 40 healthy controls were evaluated using Real-Time PCR technique. The sources of bacteria in the bloodstream of the patients suffering from septicemia were determined using microbial cultures.
Results
The results showed that mRNA levels of MDA5 and RIG-1 were significantly increased in the patients when compared to healthy controls. The results also revealed that the patients were infected with four bacteria including Escherichia coli, Staphylococcus aureus, Acinetobacter baumannii and Pseudomonas aeruginosa. mRNA levels of MDA5 and RIG-1 did not differ among patients with various bacterial infections.
Conclusion
Based on the results it seems that MDA5 and RIG-1 are the main intracellular immunity against the bacteria during septicemia and could be considered for their roles in induction of immunity.

Keywords: Septicemia; MDA5; RIG-1; Innate immunity; Inflammation

Received: September 4, 2017; Revised: November 29, 2017; Accepted: January 31, 2018; Published: November 1, 2018  Show citation