J Appl Biomed 16:387-393, 2018 | DOI: 10.1016/j.jab.2018.02.004

Genetic association of Tumour necrosis factor alpha, Interleukin-18 and Interleukin 1 beta with the risk of coronary artery disease: A case-control study outcome from Kashmir

Nuzhat Shaheen Khana, Mohammad Sultan Allaib, Bushra Nissara, Niyaz Ahmad Naykooc, Iqra Hameeda, Misbah Majida, Aaliya Bhata, Falaque ul Afshana, Bashir Ahmad Ganaid,*
a University of Kashmir, School of Biological Sciences, Department of Biochemistry, Srinagar, J&K, India
b Sher-I-Kashmir Institute of Medical Sciences, Department of Cardiology, Srinagar, J&K, India
c Government of Jammu and Kashmir, Department of Higher Education, Srinagar, J&K, India
d University of Kashmir, Centre of Research for Development, Srinagar, J&K, India

Coronary artery disease (CAD) is a clinical manifestation of atherosclerosis in the arteries supplying myocardium. Inflammation is the cornerstone in the development and progression of atherosclerosis. Amongst the various biomolecules tumour necrosis factor-α (TNF-α), interleukin-18 (IL-18) and interleukin-1β (IL-1β) build an inflammatory bionetwork in developing the disease. In this study we investigated the association of TNF-α SNPs [-308G/A (rs1800629), -1031T/C (rs1799964), -863C/A (rs1800630)]; IL-18 [-137G/C (rs187238)] and IL-1β SNPs [+3954C/T (rs1143634), -31C/T (rs1143627), and -511C/T (rs16944)] with coronary artery disease risk in Kashmiri population. A total of 200 cases and 260 controls were recruited in the study. Logistic regression analysis was done to investigate the association between SNPs and CAD risk. In case of TNF-α, the -308G/A-A/A and -863A/A showed an association with disease while -1031T/C was found to have an inverse relation. The IL-18-137G/C showed no statistically significant difference between controls and cases. For IL-1β the +3954C/T and -31C/T SNP variants showed no disease association while -511T/T showed significant association. Haplotypic analysis revealed the haplotype ATCGCC and GTACCTC to be associated with CAD risk and GTCGTTT, in particular, showing a profound association. Overall, our study suggests that TNF-α and IL-1β promoter polymorphisms may act as genetic risk factors in developing the coronary artery disease.

Keywords: Inflammation; Coronary artery disease; Kashmiri; Polymorphism; Biomarkers

Received: June 4, 2017; Revised: January 27, 2018; Accepted: February 20, 2018; Published: November 1, 2018  Show citation

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Khan NS, Sultan Allai M, Nissar B, Naykoo NA, Hameed I, Majid M, et al.. Genetic association of Tumour necrosis factor alpha, Interleukin-18 and Interleukin 1 beta with the risk of coronary artery disease: A case-control study outcome from Kashmir. J Appl Biomed. 2018;16(4):387-393. doi: 10.1016/j.jab.2018.02.004.
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