J Appl Biomed 17:39, 2019 | DOI: 10.32725/jab.2018.001

A cardiac troponin I study in a minimally invasive myocardial infarction canine model

Reyaj Mikrani¹, Cuilan Liang¹, Muhammad Naveed¹, Asghar Ali Kamboh², Muhammad Abbas¹, Birendra Chaurasiya³, Li Xue¹, Zhou Xiaohui^1,4,5,*

¹ China Pharmaceutical University, School of Basic Medicine and Clinical Pharmacy, Department of Clinical Pharmacy, Nanjing, China

² Sindh Agriculture University, Faculty of Animal Husbandry and Vet. Sciences, Department of Veterinary Microbiology, Tandojam, Pakistan

³ China Pharmaceutical University, Center for Research Development and Evaluation of Pharmaceutical Excipients and Generic Drugs, Nanjing, China

⁴ Zhongda Hospital affiliated to Southeast University, Department of Surgery, Nanjing, China

⁵ Nanjing Shuiximen Hospital, Department of Surgery, Nanjing, China

Cardiac troponin I (cTnI) is an important biomarker of acute myocardial infarction (MI) in animals and human beings. Nevertheless, no immunohistochemical study has been reported about the pattern of myocardial cTnI egression in a minimally invasive model. The present study intended to establish a minimally invasive model of MI and to evaluate the distribution of cTnI. Twelve Mongrel dogs were divided into 2 groups (n = 6): experimental and sham-operated group. Three incisions were made on the left thoracic wall, left anterior descending (LAD) of coronary artery was identified and titanium nips were clamped by video-assisted thoracoscopy surgery (VATS). Series of electrocardiograms (ECG) and biochemical analyses of blood samples - oxidatively modified proteins (OMP), creatine kinase (CK), and cTnI were performed. Furthermore, Masson's trichrome staining was used to observe the histopathology of cardiac myocytes, while immunohistochemistry was done to observe cTnI egression from myocardium. ECG showed elevated ST-segment, whereas OMP, CK and cTnI level increased remarkably and declined to baseline subsequently in the model group throughout study period. Masson's trichrome staining of model group showed a large amount of collagen deposition in the fibrotic area as compared to control group. In immunohistochemical staining, no loss of cTnI staining was observed in non-necrotic myocardium, meanwhile, a great loss was observed in necrotic myocardium. An exception was the myocardium of cardiac apex, where loss of cTnI was visible even in non-necrotic myocardium. All these results revealed that loss of cTnI occurs not only in the necrotic myocardium but also in so-called non-necrotic myocardium of minimally invasive MI model through VATS.

Keywords: Cardiac troponin I; Heart failure; Histopathology; Immunohistochemistry; Myocardial infarction; Video-assisted thoracoscopic surgery

Grants and funding:

This work was supported by the National Nature Science Foundation of China (Grant #. 30973003 and 30901993) and Administration of TCM (Grant #. LZ11093) Jiangsu Province, and this work was sponsored by Qing Lan Project and National Found for Fostering Talents of Basic Science (NFFTBS) (Grant #. J1030830).

Conflicts of interest:

All authors declare no conflict of interests regarding the content of the paper.

Received: March 19, 2018; Accepted: September 18, 2018; Prepublished online: November 23, 2018; Published: March 19, 2019  Show citation