J Appl Biomed 17:115-124, 2019 | DOI: 10.32725/jab.2019.010

Versatile biomedical potential of biosynthesized silver nanoparticles from Acacia nilotica bark

Geeta Arya1, R. Mankamna Kumari1, Richa Pundir2, Sreemoyee Chatterjee2, Nidhi Gupta2, Ajeet Kumar3, Ramesh Chandra4, Surendra Nimesh1,*
1 Central University of Rajasthan, School of Life Sciences, Department of Biotechnology, Ajmer, Rajasthan, India
2 The IIS University, Department of Biotechnology, Gurukul Marg, SFS, Mansarovar, Jaipur, Rajasthan, India
3 Clarkson University, School of Arts and Sciences, Department of Chemistry and Biomolecular Science, Potsdam, New York, USA
4 University of Delhi, Faculty of Science, Department of Chemistry, Delhi, India

The present study reports the development of potent silver nanoparticles (AgNPs) using bark extract of Acacia nilotica and evaluation of its wound healing, anti-biofilm, anti-cancer and anti-microbial activity. Stable, small sized nanoparticles with spherical morphology were obtained after significant optimization studies that was evaluated through UV-visible spectroscopy. Thereafter, physicochemical characterization of biosynthesized AgNPs was carried out through DLS and FESEM for evaluation of size. EDAX and FTIR were carried out for the evaluation of composition and possible functional groups involved in the reduction and capping of AgNPs. The antibacterial potential was investigated through disc diffusion assay against Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa). Further, the Congo Red Assay (CRA) successfully revealed the anti-biofilm activity against Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Proteus vulgaris (P. vulgaris), Pseudomonas aeruginosa (P. aeruginosa). Alamar blue assay was conducted in A549 cells to reveal the remarkable anticancer activity of biosynthesized AgNPs that resulted in a very appreciable manner. Further, the wound healing activity of AgNPs can heal the excised wound of mice up-to 100% within 15 days. All these studies suggested that our biosynthesized AgNPs possess versatile biomedical application.

Keywords: Acacia nilotica; antibacterial; antibiofilm; anticancer; silver nanoparticles; wound healing
Grants and funding:

Geeta Arya acknowledges for Senior Research Fellowship (SRF) from CSIR, Government of India and R. Mankamna Kumari acknowledges for SRF from ICMR, Government of India. Surendra Nimesh acknowledges the financial assistance from Department of Biotechnology (DBT) (grant no. 6242-P82/ RGCB/PMD/DBT/SNMH/2015), Government of India.

Conflicts of interest:

The authors declare that they have no competing interests.

Received: July 3, 2018; Accepted: June 3, 2019; Prepublished online: June 17, 2019; Published: June 28, 2019  Show citation

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Arya G, Mankamna Kumari R, Pundir R, Chatterjee S, Gupta N, Kumar A, et al.. Versatile biomedical potential of biosynthesized silver nanoparticles from Acacia nilotica bark. J Appl Biomed. 2019;17(2):115-124. doi: 10.32725/jab.2019.010. PubMed PMID: 34907733.
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