Journal of Applied Biomedicine, 2019 (vol. 17), issue 2
14-3-3ζ protein protects against brain ischemia/reperfusion injury and induces BDNF transcription after MCAO in rat
Naeemeh Khalesi, Mojgan Bandehpour, Mohammad Reza Bigdeli, Hassan Niknejad, Ali Dabbagh, Bahram Kazemi
J Appl Biomed 17:99-106, 2019 | DOI: 10.32725/jab.2019.007 
Brain ischemia is a leading cause of death and disability worldwide that occurs when blood supply of the brain is disrupted. Brain-derived neurotrophic factor (BDNF) is a protective factor in neurodegenerative conditions. Nevertheless, there are some problems when exogenous BDNF is to be used in the clinic. 14-3-3ζ is a pro-survival highly-expressed protein in the brain that protects neurons against death. This study evaluates 14-3-3ζ effects on BDNF transcription at early time point after ischemia and its possible protective effects against ischemia damage. Human 14-3-3ζ protein was purified after expression. Rats were assigned into...
Methylene blue elicits non-genotoxic H2O2 production and protects brain mitochondria from rotenone toxicity
Artem P. Gureev, Ekaterina A. Shaforostova, Denis A. Laver, Victoria G. Khorolskaya, Mikhail Yu. Syromyatnikov, Vasily N. Popov
J Appl Biomed 17:107-114, 2019 | DOI: 10.32725/jab.2019.008
Methylene blue (MB) is a promising compound with a broad range of neuroprotective activity. One of therapeutic effects is the activation of mitochondrial biogenesis via Nrf2/ARE signaling cascade. Probably, mild oxidative stress caused by MB-depended H2O2 production is a trigger for activation of this signaling cascade. So mechanistically, MB can be regarded as prooxidant. We investigated the dose-dependent H2O2 production in intact brain mitochondria and showed the increase in the H2O2 production after adding as little as 50 nM MB. We have not found genotoxic effect of therapeutic concentration of MB to mitochondrial genome. 100 μM MB selectively...
Versatile biomedical potential of biosynthesized silver nanoparticles from Acacia nilotica bark
Geeta Arya, R. Mankamna Kumari, Richa Pundir, Sreemoyee Chatterjee, Nidhi Gupta, Ajeet Kumar, Ramesh Chandra, Surendra Nimesh
J Appl Biomed 17:115-124, 2019 | DOI: 10.32725/jab.2019.010
The present study reports the development of potent silver nanoparticles (AgNPs) using bark extract of Acacia nilotica and evaluation of its wound healing, anti-biofilm, anti-cancer and anti-microbial activity. Stable, small sized nanoparticles with spherical morphology were obtained after significant optimization studies that was evaluated through UV-visible spectroscopy. Thereafter, physicochemical characterization of biosynthesized AgNPs was carried out through DLS and FESEM for evaluation of size. EDAX and FTIR were carried out for the evaluation of composition and possible functional groups involved in the reduction and capping of AgNPs....
Cross talk between redox signalling and metabolic activity of osteoblasts and fibroblasts in the presence of hydroxyapatite-based biomaterials influences bone regeneration
Ewa Ambrożewicz, Grażyna Tokajuk, Marta Muszyńska, Ilona Zaręba, Elżbieta Skrzydlewska
J Appl Biomed 17:125-135, 2019 | DOI: 10.32725/jab.2019.004
Regeneration of bone tissue defects that result from metabolic disorders, including periodontal diseases, can be supported by biomaterials based on hydroxyapatite. Despite of good biocompatibility of biomaterials they can cause oxidative stress and inflammatory processes as a result of mechanical interaction with surrounding tissues. Because osteoblasts are responsible for bone regeneration process in which gingival fibroblasts may also participate, the aim of the work was to investigate the influence of hydroxyapatite-based biomaterials (allogeneic and xenogeneic) and biomaterials combined with enamel matrix derivative (Emdogain) on osteoblast and...
Screening of the chemoprotective effect of 13 compounds and their mixtures with sodium 2-mercaptoethanesulfonate against 2-chloroethyl ethyl sulfide
Petr Jost, Jaroslav Pejchal *, Tomas Kucera, Lubica Muckova, Rudolf Stetina
J Appl Biomed 17:136-145, 2019 | DOI: 10.32725/jab.2019.009
2-chloroethyl ethyl sulfide (CEES) is a vesicant agent, commonly referred to half mustard due to its ability to form monofunctional adducts with DNA. In this study, we evaluated the chemoprotective potential of 13 compounds and their mixtures with sodium 2-mercaptoethanesulfonate (MESNA) against CEES-induced geno- and cytotoxicity in human lung cell line A-549. MESNA, L-glutathione (GSH), thiourea, sodium thiosulfate, hexamethylenetetramine, 4-acetamidophenol, asoxime dichloride (HI-6), N-acetyl-L-cysteine (NAC), sodium pyruvate, myo-inositol, 3-aminobenzamide (3-AB), nicotinamide, and Nω-nitro-L-arginine methyl ester hydrochloride...
Review Article
MR compatible ergometers for dynamic 31P MRS
Petr Sedivy, Monika Dezortova, Jan Rydlo, Miloslav Drobny, Martin Krąąák, Ladislav Valkovič, Milan Hajek
J Appl Biomed 17:91-98, 2019 | DOI: 10.32725/jab.2019.006
Magnetic Resonance (MR) compatible ergometers are specialized ergometers used inside the MR scanners for the characterization of tissue metabolism changes during physical stress. They are most commonly used for dynamic phosphorous magnetic resonance spectroscopy (31P MRS), but can also be used for lactate production measurements, perfusion studies using arterial spin labelling or muscle oxygenation measurements by blood oxygen dependent contrast sequences. We will primarily discuss the importance of ergometers in the context of dynamic 31P MRS. Dynamic 31P MRS can monitor muscle fatigue and energy reserve during muscle contractions as well as the dynamics...