J Appl Biomed 17:175-183, 2019 | DOI: 10.32725/jab.2019.016

Correlation of adenosine deaminase operating under nitro-oxidative stress with tumor and vascularization in patients with advanced gallbladder carcinoma

Nabila Tounsi1,*, Bahia Djerdjouri1, Chafik Bouzid2, Kamel Bentabak2
1 University of Sciences and Technology Houari Boumediene, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, Algiers, Algeria
2 Mustapha Pacha Hospital, Public Health Center Pierre and Marie Curie, Department of Oncological Surgery A, Algiers, Algeria

This study investigates serum redox status and adenosine catabolism markers in relation to tumor and angiogenesis, in patients with gallbladder carcinoma (GBC). The level of adenosine deaminase (ADA) and xanthine oxidase (XO) activities, nitrites (NO2-), glutathione (GSH) and malondialdehyde (MDA) were measured in sera of 40 GBC patients and 40 healthy donors. In parallel, 15 tumors at TNM stage IV were scored for CD34 expression and microvessel density (MVD). The results showed that XO and ADA activities, nitrites and MDA levels enhanced by 1.26 (p < 0.01), 2.69, 2.0, and 3.2-fold (p < 0.001), respectively, while those of GSH decreased by 44.6% (p < 0.001). According to receiver operating characteristic (ROC) curve, the optimal cut-off for XO, ADA, MDA, GSH and nitrites were 5.41U/l, 17.02 U/l, 3.72 μM, 36.91 μM and 21.21 μM, respectively. Spearman correlation revealed that ADA activity correlated to nitrites levels (r = 0.3419, p < 0.05) and XO activity (r = 0.5487, p < 0.001). Multivariate binary logistic regression analysis revealed that MDA (OR = 5.78, p < 0.05), ADA (OR = 1.28, p < 0.001) and XO (OR = 2.81, p < 0.05) correlated positively to GBC. CD34 was up expressed in 73.3% of tumors at intermediate to high levels. Multiple regression analysis showed that ADA affected MVD (r = 0.604, p < 0.01). The results suggest that high MDA/GSH ratio is a potential biomarker of GBC. In addition, the oxidative adenosine catabolism indicated that active purine salvage pathway could support tumor progression by sustaining angiogenesis.

Keywords: Adenosine deaminase; Gallbladder adenocarcinoma; Microvessel density; Nitro-oxidative stress; Xanthine oxidase
Conflicts of interest:

The authors have no conflict of interests to declare.

Received: March 14, 2019; Accepted: August 20, 2019; Prepublished online: September 17, 2019; Published: September 18, 2019  Show citation

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Tounsi N, Djerdjouri B, Bouzid C, Bentabak K. Correlation of adenosine deaminase operating under nitro-oxidative stress with tumor and vascularization in patients with advanced gallbladder carcinoma. J Appl Biomed. 2019;17(3):175-183. doi: 10.32725/jab.2019.016. PubMed PMID: 34907699.
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