J Appl Biomed 18:70-79, 2020 | DOI: 10.32725/jab.2020.010

Cichorium intybus attenuates Streptozotocin-induced pancreatic β-cell damage by inhibiting NF-κB activation and oxidative stress

Ramya Devi KT *, Nageswaran Sivalingam
SRM Institute of Science and Technology, Faculty of Engineering and Technology, School of Bioengineering, Department of Biotechnology, Tamil Nadu, India

The aqueous extract of Cichorium intybus (CIE) leaves have shown the properties of protecting against pancreatic β-cell damage by streptozotocin (STZ), but the molecular mechanisms of its protection are not completely elucidated yet. Our current study focuses on elucidating the mechanisms of these preventive effects of CIE in MIN6 cells and an in-vivo model of Wistar rats. CIE offers protection against STZ in MIN6 cells by reducing the pro-oxidants and increasing the activity of the antioxidant enzymes. In vitro results also indicated that CIE inhibited cytotoxicity, reduced Reactive oxygen species (ROS), maintained glucose-stimulated insulin secretion and reduced NF-κB p65 translocation into the nucleus. The group administered with a 250 mg/kg dose of CIE in vivo has shown an ability to maintain blood glucose level and also to preserve the number and morphology of pancreatic islets when compared to the diabetic group treated with STZ. Probably, active compounds like quercetin, rutin, and catechin present in CIE, preserve the integrity of pancreatic islets thereby protecting β-cells from the adverse effects of STZ.

Keywords: Cichorium intybus; Cytotoxicity; MIN6; NF-κB; pancreatic β-cell; Streptozotocin
Grants and funding:

Financial assistance and infrastructure support was provided by SRM Institute of Science and Technology.

Conflicts of interest:

The authors declare no conflict of interests

Received: January 21, 2019; Revised: July 11, 2020; Accepted: July 11, 2020; Prepublished online: August 12, 2020; Published: August 27, 2020  Show citation

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Devi KT R, Sivalingam N. Cichorium intybus attenuates Streptozotocin-induced pancreatic β-cell damage by inhibiting NF-κB activation and oxidative stress. J Appl Biomed. 2020;18(2-3):70-79. doi: 10.32725/jab.2020.010. PubMed PMID: 34907728.
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