J Appl Biomed 19:228-233, 2021 | DOI: 10.32725/jab.2021.022

Genetic studies of nephrotic syndrome in Egyptian children

Rehab Elmougy
Mansoura University, Faculty of Science, Chemistry Department, Mansoura, Egypt

Introduction: Nephrotic syndrome (NS) might be caused by a kidney disorder or it can be a secondary disease. Untreated or resistant to treatment, NS stimulates glomerular damage that reduces the kidney function. This reduction leads to the end stage of renal failure. Therefore, it is very important to diagnose NS early, with the aim of inhibiting or lessening its associated morbidity and mortality.

Methods: Gene polymorphism analysis for the three genes eNOS 27 bp VNTR, GSTP1 and IL-10(1082 G/A) were checked in 98 children with NS and 101 control subjects.

Results: eNOS 27 bp VNTR genotypes and alleles are significantly different in the group of 98 children with NS compared to the 101 control subjects. The frequencies of ab and bb genotypes are significantly lower in patients than in the control group (ab: 17.2% vs. 22.8%; OR: 0.19; 95% CI: 0.06-0.58; p = 0.0026 & bb: 54.7% vs. 70.3%; OR: 0.19; 95% CI: 0.07-0.5; p = 0.0004). However, neither GSTP1 nor IL-10(1082 G/A) genotypes showed any significant difference in both groups.

Conclusions: eNOS 27 bp VNTR gene might be considered as an independent risk factor in the early prediction of nephrotic syndrome incidence, which may help prevent/reduce the occurrence of other complications associated with the late diagnosis and treatment of the disease.

Keywords: eNOS 27 bp VNTR; GSTP1 and IL-10(1082 G, A); Nephrotic syndrome
Conflicts of interest:

The author has no conflict of interests to declare.

Received: September 27, 2020; Revised: July 11, 2021; Accepted: September 22, 2021; Prepublished online: October 12, 2021; Published: December 6, 2021  Show citation

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Elmougy R. Genetic studies of nephrotic syndrome in Egyptian children. J Appl Biomed. 2021;19(4):228-233. doi: 10.32725/jab.2021.022. PubMed PMID: 34907742.
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    References

    1. Agrawal S, Tripathi G, Khan F, Sharma R, Baburaj VP (2007). Relationship between GSTs Gene Polymorphism and Susceptibility to End Stage Renal Disease among North Indians. Ren Fail 29(8): 947-953. DOI: 10.1080/08860220701641314. Go to original source... Go to PubMed...
    2. Ahluwalia TS, Ahuja M, Rai TS, Kohli HS, Sud K, Bhansali A, Khullar M (2008). Endothelial nitric oxide synthase gene haplotypes and diabetic nephropathy among Asian Indians. Mol Cell Biochem 314(1-2): 9-17. DOI: 10.1007/s11010-008-9759-8. Go to original source... Go to PubMed...
    3. Buraczynska M, Ksiazek P, Zaluska W, Nowicka T, Ksiazek A (2004). Endothelial nitric oxide synthase gene intron 4 polymorphism in patients with end-stage renal disease. Nephrol Dial Transplant 19(9): 2302-2306. DOI: 10.1093/ndt/gfh077. Go to original source... Go to PubMed...
    4. Channon KM, Qian HS, George SE (2000). Nitric Oxide Synthase in Atherosclerosis and Vascular Injury Insights: From Experimental Gene Therapy. Arterioscler Thromb Vasc Biol 20(8): 1873-1881. DOI: 10.1161/01.atv.20.8.1873. Go to original source... Go to PubMed...
    5. Dudnyk V, Zvenigorodska G, Zborovska O, Vyzhga I, Moskaliuk O (2019). Evaluation of gene polymorphism of IL-1B and IL-10 in children with Nephrotic Syndrome. Georgian Med News 294: 68-71.
    6. Dumas De La Roque C, Prezelin-Reydit M, Vermorel A, Lepreux S, Deminière C, Combe C, Rigothier C (2018). Idiopathic Nephrotic Syndrome: Characteristics and Identification of Prognostic Factors. J Clin Med 7(9): 265. DOI: 10.3390/jcm7090265. Go to original source... Go to PubMed...
    7. Dursun H, Noyan A, Matyar S, Buyukcelik M, Soran M, Cengiz N, et al. (2013). Association of eNOS gene intron 4 a/b VNTR polymorphisms in children with nephrotic syndrome. Gene 522(2): 192-195. DOI: 10.1016/j.gene.2013.03.080. Go to original source... Go to PubMed...
    8. Elsaid A, Abdel-Aziz AF, Elmougy R, Elwaseef AM (2014). Association of polymorphisms G (-174) C in IL-6 gene and G (-1082) A in IL-10 gene with traditional cardiovascular risk factors in patients with coronary artery disease. Indian J Biochem Biophys 51(4): 282-292.
    9. Gsur A, Haidinger G, Hinteregger S, Bernhofer G, Schatzl G, Maderbacher S, et al. (2001). Polymorphism of glutathione S-transferases genes (GSTP1, GSTM1 and GSTT1) and prostate cancer risk. Int J Cancer 95(3): 152-155. DOI: 10.1002/1097-0215(20010520)95:33.0.co;2-s. Go to original source...
    10. Gusti AMT, Qusti SY, Bahijri SM, Toraih EA, Bokhari S, Attallah SM, et al. (2021). Glutathione S-Transferase (GSTT1 rs17856199) and Nitric Oxide Synthase (NOS2 rs2297518) Genotype Combination as Potential Oxidative Stress-Related Molecular Markers for Type 2 Diabetes Mellitus. Diabetes Metab Syndr Obes 14: 1385-1403. DOI: 10.2147/DMSO.S300525. Go to original source... Go to PubMed...
    11. Hill AJ, Stone DE, Elliott JP, Gerkin RD, Ingersoll M, Cook CR (2016). Management of Nephrotic Syndrome in the Pregnant Patient. J Reprod Med 61(11-12): 557-561. Go to PubMed...
    12. Jafar T, Agrawal S, Mahdi AA, Sharma RK, Awasthi S, Agarwa GG (2011). Cytokine Gene Polymorphism in Idiopathic Nephrotic Syndrome Children. Indian J Clin Biochem 26(3): 296-302. DOI: 10.1007/s12291-011-0126-2. Go to original source... Go to PubMed...
    13. Kodner C (2016). Diagnosis and Management of Nephrotic Syndrome in Adults. Am Fam Physician 93(6): 479-485. Go to PubMed...
    14. Krawiec P, Paw³owska-Kamieniak A, Pac-Ko¿uchowska E (2021). Interleukin 10 and interleukin 10 receptor in paediatric inflammatory bowel disease: from bench to bedside lesson. J Inflamm 18(1): 13. DOI: 10.1186/s12950-021-00279-3. Go to original source... Go to PubMed...
    15. Mehmetoğlu I, Yilmaz G, Kurban S, Acar H, Düzenli MA (2010). Investigation of eNOS gene intron 4 A/B VNTR and intron 23 polymorphisms in patients with essential hypertension. Turk J Med Sci 40(3): 479-484. DOI: 10.3906/sag-0809-31. Go to original source...
    16. Murata M, Shiraishi T, Fukutome K, Watanabe M, Nagao M, Kubota Y, et al. (1998). Cytochrome P4501A1 and glutathione Stransferases M1 genotypes as risk factors for prostate cancer in Japan. Jpn J Clin Oncol 28(11): 657-660. DOI: 10.1093/jjco/28.11.657. Go to original source... Go to PubMed...
    17. Naing C, Htet NH, Basavaraj AK, Nalliah S (2018). An association between IL-10 promoter polymorphisms and diabetic nephropathy: a meta-analysis of case-control studies. J Diabetes Metab Disord 17(2): 333-343. DOI: 10.1007/s40200-018-0349-3. Go to original source... Go to PubMed...
    18. Polina ER, da Silva Pereira BL, Crispimb D, Sbruzzi RC, Canani LH, Dos Santos KG (2017). Association of -1082A>G Polymorphism in the Interleukin-10 Gene with Estimated Glomerular Filtration Rate in Type 2 Diabetes. Kidney Blood Press Res 42(6): 1164-1174. DOI: 10.1159/000485863. Go to original source... Go to PubMed...
    19. Raina R, Krishnappa V (2019). An update on LDL apheresis for nephrotic syndrome. Pediatr Nephrol 34(10): 1655-1669. DOI: 10.1007/s00467-018-4061-9. Go to original source... Go to PubMed...
    20. Salimi S, Nakhaee A, Jafari M, Jahantigh D, Sandooghi M, Zakeri Z, et al. (2015). Combination Effect of GSTM1, GSTT1 and GSTP1 Polymorphisms and Risk of Systemic Lupus Erythematosus. Iran J Public Health 44(6): 814-821.
    21. Sharda SV, Gulati S, Tripathi G, Jafar T, Kumar A, Sharma RK, Agrawal S (2008). Do glutathione-S-transferase polymorphisms influence response to intravenous cyclophosphamide therapy in idiopathic nephrotic syndrome? Pediatr Nephrol 23(11): 2001-2006. DOI: 10.1007/s00467-008-0883-1. Go to original source... Go to PubMed...
    22. Shoukry A, Shalaby SM, Abdelazim S, Abdelazim M, Ramadan A, Ismail MI, Fouad M (2012). Endothelial Nitric Oxide Synthase Gene Polymorphisms and the Risk of Diabetic Nephropathy in Type 2 Diabetes Mellitus. Genet Test Mol Biomarkers 16(6): 574-579. DOI: 10.1089/gtmb.2011.0218. Go to original source... Go to PubMed...
    23. Steinhoff C, Franke KH, Golka K, Their R, Römer HC, Rötzel C, et al. (2000). Glutathione transferases isoenzyme genotypes in patients with prostate and bladder carcinoma. Arch Toxicol 74(9): 521-526. DOI: 10.1007/s002040000161. Go to original source... Go to PubMed...
    24. Tapia C, Bashir K (2020). Nephrotic Syndrome. [online] [cit. 2020-06-26]. StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing LLC. Available from: https://www.ncbi.nlm.nih.gov/books/NBK470444/
    25. Tumlin JA, Campbell KN (2018). Proteinuria in Nephrotic Syndrome: Mechanistic and Clinical Considerations in Optimizing Management. Am J Nephrol 47(Suppl. 1): 1-2. DOI: 10.1159/000481632. Go to original source... Go to PubMed...
    26. Turner DM, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, Hutchinson IV (1997). An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet 24(1): 1-8. DOI: 10.1111/j.1365-2370.1997.tb00001.x. Go to original source... Go to PubMed...
    27. Vasudevan A, Thergaonkar R, Mantan M, Sharma J, Khandelwal P, Hari P, et al. (2021). Consensus Guidelines on Management of Steroid-Resistant Nephrotic Syndrome. Indian Pediatr 58(7): 650-666. Go to original source... Go to PubMed...
    28. Wang XL, Sim AS, Badenhop RF, McCredie RM, Wilcken DE (1996). A smoking dependent risk of coronary artery disease associated with a polymorphism of the endothelial nitric oxide synthase gene. Nat Med 2(1): 41-45. DOI: 10.1038/nm0196-41. Go to original source... Go to PubMed...

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