Journal of Applied Biomedicine, 2021 (vol. 19), issue 4
Original Research Article
Does GD2 synthase (GD2S) detect cancer stem cells in blood samples of breast carcinomas?
Maryam Mansoori, Isa Abdi Rad, Alireza Mirzaei, Kevin J. Tam, Seyed Mohsen Hosseini, Rahim Mahmodlu, Fatemeh Mansouri, Leili Saeednejad Zanjani, Zahra Madjd
J Appl Biomed 19:181-189, 2021 | DOI: 10.32725/jab.2021.019 
Introduction: Cancer stem cells (CSCs) are a theorized subset of cells within the tumor that is thought to drive disease recurrence and metastatic spread. The aim of this study is to investigate mRNA and protein levels of ganglioside GD2 synthase (GD2S), in breast cancer (BC) patients. Methods: 65 PBMCs of preoperative BC patients without chemotherapy were compared to PBMCs after chemotherapy and controls. Results: GD2S were significantly higher in BC patients after chemotherapy compared to pre-chemotherapy at both mRNA and protein. GD2S was higher in pre-chemotherapy blood samples compared to control samples. Conclusions: Higher expression of GD2S...
The NF-κB pathway is critically implicated in the oncogenic phenotype of human osteosarcoma cells
Bingyi Tan, Zenong Yuan, Qingyu Zhang, Xu Xiqiang, Jun Dong
J Appl Biomed 19:190-201, 2021 | DOI: 10.32725/jab.2021.021
NF-κB is activated in a variety of human cancers. However, its role in osteosarcoma (OS) remains unknown. Here, we have elucidated the implication of NF-κB in the oncogenic phenotype of OS tumor cells. We reported that activation of NF-κB was a common event in the human OS. Inhibition of NF-κB using inhibitor Bay 11-7085 repressed proliferation, survival, migration, and invasion but increased apoptosis in 143B and MG63 OS cells, indicating that NF-κB is critically implicated in the oncogenesis of OS. Notably, Bay 11-7085 not only inactivated NF-κB but also reduced the phosphorylation of AKT via its induction of PTEN, suggesting...
Rosmarinic acid suppresses inflammation, angiogenesis, and improves paclitaxel induced apoptosis in a breast cancer model via NF3 κB-p53-caspase-3 pathways modulation
Marwa A. Mahmoud, Tark M. Okda, Gamal A. Omran, Mohammad M. Abd-Alhaseeb
J Appl Biomed 19:202-209, 2021 | DOI: 10.32725/jab.2021.024
Rosmarinic acid is a natural polyphenolic compound that is found in different plant species and used for different medicinal purposes. This study aimed to investigate the chemo-preventive effect of rosmarinic acid and evaluate its antitumor efficacy alone or in combination with Paclitaxel in breast cancer mice model. Ehrlich induced mice mammary solid tumor model was used in the study. Mice were treated with oral rosmarinic acid and intraperitoneal Paclitaxel. Inflammation, angiogenesis, and apoptosis were checked. Enzyme linked immunosorbent assay (ELISA), quantitative real time PCR, and immunohistochemical methods were performed. Rosmarinic acid...
Insulin degludec and glutamine dipeptide modify glucose homeostasis and liver metabolism in diabetic mice undergoing insulin-induced hypoglycemia
Camila Bataglini, Isabela Ramos Mariano, Sílvia Carla Ferreira Azevedo, Valder Nogueira Freire, Maria Raquel Marçal Natali, Maria Montserrat Dias Pedrosa, Rosane Marina Peralta, Anacharis B. Sá-Nakanishi, Lívia Bracht, Vilma A. Ferreira Godoy, Adelar Bracht, Jurandir Fernando Comar
J Appl Biomed 19:210-219, 2021 | DOI: 10.32725/jab.2021.025
This study investigated whether a 30-day co-treatment with 1 g/kg glutamine dipeptide (GdiP) and 1 U/kg regular (rapid acting) or 5 U/kg degludec (long acting) insulins modifies glucose homeostasis and liver metabolism of alloxan-induced type 1 diabetic (T1D) male Swiss mice undergoing insulin-induced hypoglycemia (IIH). Glycemic curves were measured in fasted mice after IIH with 1 U/kg regular insulin. One hour after IIH, the lipid profile and AST and ALT activities were assayed in the serum. Morphometric analysis was assessed in the liver sections stained with hematoxylin-eosin and glycolysis, glycogenolysis, gluconeogenesis and ureagenesis were...
Cholesterol and glucose profiles according to different fasting C-peptide levels: a cross-sectional analysis in a healthy cohort from the Czech Republic
Vladimír Kron, Miroslav Verner, Ladislav Pešl, Pavel Smetana, Jaromír Kadlec, Daniel Martiník
J Appl Biomed 19:220-227, 2021 | DOI: 10.32725/jab.2021.023
The relationship between glycaemia and lipoprotein metabolism has not been completely clarified, and slight differences may be found between local authors, trials and evaluated parameters. Therefore this cross-sectional study investigated fasting cholesterol and glucose levels along with the determination of atherogenic index in a cohort of healthy individuals from the Czech Republic in relation to their fasting C-peptide levels. Data were collected between 2009 and 2018 and a total of 3189 individuals were stratified by C-peptide reference range (260-1730 pmol/l) into three groups - below (n = 111), within (n = 2952) and above (n...
Genetic studies of nephrotic syndrome in Egyptian children
Rehab Elmougy
J Appl Biomed 19:228-233, 2021 | DOI: 10.32725/jab.2021.022
Introduction: Nephrotic syndrome (NS) might be caused by a kidney disorder or it can be a secondary disease. Untreated or resistant to treatment, NS stimulates glomerular damage that reduces the kidney function. This reduction leads to the end stage of renal failure. Therefore, it is very important to diagnose NS early, with the aim of inhibiting or lessening its associated morbidity and mortality. Methods: Gene polymorphism analysis for the three genes eNOS 27 bp VNTR, GSTP1 and IL-10(1082 G/A) were checked in 98 children with NS and 101 control subjects. Results: eNOS 27 bp VNTR genotypes and alleles are significantly different in the group of 98...
Finite element analysis of the tibial component alignment in a transverse plane in total knee arthroplasty
Roman Popescu, Stefan Cristea, Valentin Oleksik, Adrian Marius Pascu, Emil George Haritinian
J Appl Biomed 19:234-239, 2021 | DOI: 10.32725/jab.2021.020
The research aims to analyze the tibial component rotation using the finite element method by resecting the tibia in a transverse plane at an angle between 1.5° (external rotation) and -1.5° (internal rotation). We used a three-dimensional scanner to obtain the tibia's geometrical model of a cadaveric specimen. We then exported the surfaces of the tibial geometrical model through the Computer-Aided Three-dimensional Interactive Application (CATIA), which is a Computer-Aided Design (CAD) program. The CAD program three-dimensionally shaped the tibial component, polyethylene, and cement. Our analysis determined that the maximum equivalent stress is obtained...