J Appl Biomed 21:180-192, 2023 | DOI: 10.32725/jab.2023.024

Ginsenoside Rb2 improves heart failure by down-regulating miR-216a-5p to promote autophagy and inhibit apoptosis and oxidative stress

You Peng1, 2, 3, *, Bin Liao1, Yan Zhou1, Wei Zeng1
1 The First Affiliated Hospital of Hunan Normal University, Hunan Provincial People's Hospital, Center of Geriatric, Changsha, Hunan, China
2 Hunan Research Institute of Geriatrics, Changsha, Hunan, China
3 Major Chronic Disease Research Center of Hunan Provincial Geriatric Institute, Changsha, Hunan, China

Background: Ginsenoside Rb2 is beneficial in cardiovascular disease treatment, yet its role in heart failure (HF) is obscure. This study aimed to investigate the effect and mechanism of ginsenoside Rb2 on HF.

Methods: The left anterior descending branch-ligated HF rat model and oxygen-glucose deprivation/reoxygenation (OGD/R) H9c2 cell model were constructed. Ginsenoside Rb2 were applied for intervention. Heart function indexes, miR-216a-5p expression, autophagy, oxidative stress, apoptosis, cell morphology, and proliferation were detected to explore the effect of ginsenoside Rb2 on HF. Overexpression of miR-216a-5p was employed to explore the specific mechanism of ginsenoside Rb2 on HF.

Results: Ginsenoside Rb2 improved the heart function of HF rats, including the reduction of heart rate, LVEDP, and heart weight/body weight ratio, and the increase of LVSP, +dP/dtmax, -dP/dtmax, LVEF, and LVFS. It also down-regulated miR-216a-5p expression and enhanced OGD/R-induced cardiomyocyte viability. Ginsenoside Rb2 up-regulated Bcl2, LC3B II/I, and Beclin1, and down-regulated Bax, Caspase-3, and p62 in the myocardium of HF rats and OGD/R-induced H9c2 cells. Moreover, ginsenoside Rb2 increased the levels of SOD and CAT, but decreased the levels of MDA and ROS in the myocardium of HF rats and OGD/R-induced H9c2 cells. However, overexpression of miR-216a-5p promoted the apoptosis and oxidative stress of cardiomyocytes and inhibited autophagy, thus reversing the therapeutic effect of ginsenoside Rb2 on HF in vivo and in vitro.

Conclusion: Ginsenoside Rb2 demonstrated potential as a therapeutic intervention for HF by enhancing autophagy and reducing apoptosis and oxidative stress through miR-216a-5p downregulation. Further research could explore its application in clinical trials and investigate the complex mechanism networks underlying its effects.

Keywords: Apoptosis; Autophagy; Ginsenoside Rb2; Heart failure; miR-216a-5p; Oxidative stress
Grants and funding:

This study was supported by Hunan Traditional Chinese Medicine Research Program (No. E2022025) and Key Scientific Research Project of Hunan Provincial Department of Education (No. 21A0044).

Conflicts of interest:

The authors have no conflict of interest to declare regarding the present study.

Received: March 2, 2023; Revised: November 21, 2023; Accepted: December 5, 2023; Prepublished online: December 14, 2023; Published: December 18, 2023  Show citation

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Peng Y, Liao B, Zhou Y, Zeng W. Ginsenoside Rb2 improves heart failure by down-regulating miR-216a-5p to promote autophagy and inhibit apoptosis and oxidative stress. J Appl Biomed. 2023;21(4):180-192. doi: 10.32725/jab.2023.024. PubMed PMID: 38112457.
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