Journal of Applied Biomedicine, 2023 (vol. 21), issue 4
Original Research Article
Olfactory event-related potentials (OERPs) and trigeminal event-related potentials (TERPs) - a pilot study in Czech participants with normal sense of smell
Richard Holý, Karla Janoušková, Libor Vašina, Eva Maute, David Kalfeřt, Kristýna Mamiňák, Eva Augste, Jiří Hložek, Helene Schulz, David Funda, Jaromír Astl
J Appl Biomed 21:167-173, 2023 | DOI: 10.32725/jab.2023.020 
Introduction: In recent years, the evaluation of potential events related to olfactory events (OERPs) and trigeminal events (TERPs) has become increasingly important in the diagnosis of olfactory disorders. This technique is increasingly used in basic research and clinical practice to evaluate people suffering from olfactory disorders. Purpose of the study: In a pilot project of the first investigations of OERPs and TERPs in the Czech Republic, we analyse the event-related potentials of the data of normosmic participants. Methods: In the prospective study, 21 normosmic participants were enrolled for a 2-year period (5/2021-5/2023). OERPs/TERPs were...
False aneurysms of the thoracic aorta: anastomosis investigation using the inflation-extension test
Sandra Rečičárová, Hynek Chlup, Michael Jonák, Ivan Netuka
J Appl Biomed 21:174-179, 2023 | DOI: 10.32725/jab.2023.023
Introduction: False aneurysms in the thoracic aorta are dangerous complications that can occur after cardiac surgery. They often result in high mortality rates. These aneurysms are caused by damage to all layers of the aortic wall. This study aimed to pinpoint the area of the experimental specimen (native vessel, anastomosis, or prosthetic graft) with the greatest deformation, to determine whether a false aneurysm is likely to develop in the anastomotic portion. Methods: We conducted the inflation-extension test by performing eight cycles ranging from 0 to 20. The pressure sampling frequency was 100 Hz, and each cycle lasted approximately 34 seconds,...
Ginsenoside Rb2 improves heart failure by down-regulating miR-216a-5p to promote autophagy and inhibit apoptosis and oxidative stress
You Peng, Bin Liao, Yan Zhou, Wei Zeng
J Appl Biomed 21:180-192, 2023 | DOI: 10.32725/jab.2023.024
Background: Ginsenoside Rb2 is beneficial in cardiovascular disease treatment, yet its role in heart failure (HF) is obscure. This study aimed to investigate the effect and mechanism of ginsenoside Rb2 on HF. Methods: The left anterior descending branch-ligated HF rat model and oxygen-glucose deprivation/reoxygenation (OGD/R) H9c2 cell model were constructed. Ginsenoside Rb2 were applied for intervention. Heart function indexes, miR-216a-5p expression, autophagy, oxidative stress, apoptosis, cell morphology, and proliferation were detected to explore the effect of ginsenoside Rb2 on HF. Overexpression of miR-216a-5p was employed to explore the specific...
Naringin inhibits P2X4 receptor expression on satellite glial cells in the neonatal rat dorsal root ganglion
Hongji Wang, Lisha Chen, Juping Xing, Xiangchao Shi, Changshui Xu
J Appl Biomed 21:193-199, 2023 | DOI: 10.32725/jab.2023.021
Naringin inhibits inflammation and oxidative stress, the P2 purinoreceptor X4 receptor (P2X4R) is associated with glial cell activation and inflammation, the purpose of this study is to investigate the effects of naringin on P2X4 receptor expression on satellite glial cells (SGCs) and its possible mechanisms. ATP promoted the SGC activation and upregulated P2X4R expression; naringin inhibited SGC activation, decreased expression of P2X4R, P38 MAPK/ERK, and NF-κB, and reduced levels of Ca2+, TNF-α, and IL-1β in SGCs in an ATP-containing environment. These findings suggest that naringin attenuates the ATP-induced SGC activation and reduces...
Effect of Usnea longissima ethyl acetate extract on acute oxidative and inflammatory lung damage from Staphylococcus aureus infection in rats
Bulent Dabanlioglu, Bahadir Suleyman, Renad Mammadov, Bulent Yavuzer, Sumeyye Akyuz, Onder Akkas, Behzad Mokhtare, Emine Akyuz Turumtay, Durdu Altuner, Taha Abdulkadir Coban, Halis Suleyman
J Appl Biomed 21:200-207, 2023 | DOI: 10.32725/jab.2023.022
The role of oxidants and proinflammatory cytokines in the pathogenesis of pneumonia caused by Staphylococcus aureus (S. aureus) has been demonstrated. The present study aims to investigate the protective effect of ethyl acetate extract (EtOAc) obtained from Usnea longissima (UL) against acute oxidative and inflammatory lung damage due to S. aureus infection in rats. Albino Wistar-type male rats were divided into three groups: Healthy (HG), S. aureus inoculated (SaG), and S. aureus inoculated + ULEtOAc administered (SUL). SaG (n = 6) and SUL (n = 6) group rats' left nostrils (excluding HG) were...
Shenhuang plaster enhances intestinal anastomotic healing in rabbits through activation of the TGF-β and Hippo/YAP signaling pathways
Fengchun Xiao, Chenchen Zhu, Xing Wei, Guiping Chen *, Xiaohong Xu *
J Appl Biomed 21:208-217, 2023 | DOI: 10.32725/jab.2023.018
Although many efforts have been made to improve management strategies and diagnostic methods in the past several decades, the prevention of anastomotic complications, such as anastomotic leaks and strictures, remain a major clinical challenge. Therefore, new molecular pathways need to be identified that regulate anastomotic healing, and to design new treatments for patients after anastomosis to reduce the occurrence of complications. Rabbits were treated with a MST1/2 inhibitor XMU-XP-1, a Chinese medicine formula Shenhuang plaster (SHP) or a control vehicle immediately after surgery. The anastomotic burst pressure, collagen deposition, and hydroxyproline...
Homospisulosine induced apoptosis in cervical carcinoma cells is associated with phosphorylation of Bcl-2 and up-regulation of p27/Kip1
Martina Bago Pilátová, Natália Nosálová, Gabriela Očkajáková, Martin Kello, Klaudia Kotorová, Peter Takáč, Peter Petik, Peter Bohuš, Kvetoslava Stanková, Miroslava Martinková, Roman Mezencev
J Appl Biomed 21:218-227, 2023 | DOI: 10.32725/jab.2023.019
Spisulosine (1-deoxysphinganine) is a sphingoid amino alcohol isolated from the sea clams that showed potent antiproliferative activity against a broad spectrum of solid tumors but failed in clinical trials due to neurotoxicity. However, its structural similarity to other bioactive sphingoids, interesting mode of action, and appreciable potency against cancer cells make it a suitable lead for future anticancer drug development. The present study was conducted to elucidate mechanisms of the antiproliferative/cytotoxic effects of newly synthesized spisulosine analog homospisulosine (KP7). The evaluation was performed on cervical carcinoma cells, representing...