J Appl Biomed 21:193-199, 2023 | DOI: 10.32725/jab.2023.021

Naringin inhibits P2X4 receptor expression on satellite glial cells in the neonatal rat dorsal root ganglion

Hongji Wang¹, Lisha Chen¹, Juping Xing¹, Xiangchao Shi¹, Changshui Xu^{1, 2, 3, 4, *}

¹ Basic Medical College of Nanchang University, Department of Physiology, Nanchang, 330006, P.R. China

² Jiangxi Provincial Key Laboratory of autonomic nervous function and disease, Nanchang, 330006, P.R. China

³ Jiangxi Medical College, School of Clinical Medicine, Shangrao, 334000, P.R. China

⁴ Jiangxi Medical College, The First Affiliated Hospital, Shangrao, 334000, P.R. China

Naringin inhibits inflammation and oxidative stress, the P2 purinoreceptor X4 receptor (P2X4R) is associated with glial cell activation and inflammation, the purpose of this study is to investigate the effects of naringin on P2X4 receptor expression on satellite glial cells (SGCs) and its possible mechanisms. ATP promoted the SGC activation and upregulated P2X4R expression; naringin inhibited SGC activation, decreased expression of P2X4R, P38 MAPK/ERK, and NF-κB, and reduced levels of Ca2+, TNF-α, and IL-1β in SGCs in an ATP-containing environment. These findings suggest that naringin attenuates the ATP-induced SGC activation and reduces P2X4R expression via the Ca2+-P38 MAPK/ERK-NF-κB pathway.

Keywords: Glial cell activation; Inflammation; Naringin; P2X4R; Satellite glial cells

Grants and funding:

This study was supported by Jiangxi Provincial Natural Science Foundation (grant Nos. 20232ACB206020 and 20202BAB206027), and the High-level and High-Skilled Leading Talent Training Project of Jiangxi Province in 2021 (grant No. 2022233 Jiangxi Province Human Resources and Social Security Department) also supported this work.

Conflicts of interest:

The authors have no conflict of interest to declare.

Received: March 4, 2023; Revised: August 30, 2023; Accepted: November 27, 2023; Prepublished online: December 4, 2023; Published: December 18, 2023  Show citation