J Appl Biomed 22:23-32, 2024 | DOI: 10.32725/jab.2024.005

Dictyophora indusiata polysaccharide mediates priming of the NLRP3 inflammasome activation via TLR4/ NF-κB signaling pathway to exert immunostimulatory effects

Youyi Liu1, Huanxiao Zhang1, Yuxuan Li1, Hanqian Zha1, Yujie Gao1, Hui Chen2, Yalin Wang1, Tongxin Zhou2, *, Chao Deng1, *
1 Jiangnan University, Wuxi School of Medicine, Wuxi, Jiangsu 214122, P.R. China
2 Yixing Hospital of Traditional Chinese Medicine, Wuxi, Jiangsu 214200, P.R. China

Dictyophora indusiata, commonly known as bamboo fungus, is a type of edible mushroom that is highly popular worldwide for its rich flavor and nutritional value. It is also recognized for its pharmaceutical efficacy, with medicinal benefits attributed to its consumption. One of the most important components of Dictyophora indusiata is polysaccharide, which has been acknowledged as a promising regulator of biological response due to its immunostimulatory and anti-inflammatory properties. However, the specific roles of polysaccharide in modulating the NOD-like receptor protein 3 (NLRP3) inflammasome activation within macrophages remain relatively under-researched. To investigate this further, the mechanism by which Dictyophora indusiata polysaccharide (DIP) exerts its immunostimulatory activity in RAW 264.7 macrophages was analyzed. Results indicated that DIP has the potential to facilitate the priming of NLRP3 inflammasome activation by enhancing TLR4 expression, phosphorylation of IκB-α, and nuclear translocation of NF-κB p65 subunit. It was noted that DIP was unable to mediate the second step of NLRP3 inflammasome activation. The findings of this study provide compelling evidence that DIP has immunomodulatory effects by modulating the NLRP3 inflammasome in RAW264.7 macrophages.

Keywords: Dictyophora indusiata polysaccharide; Immunostimulatory activity; Macrophage; NLRP3 inflammasome
Grants and funding:

This work was supported by grants from the Postdoctoral Science Foundation of China (2021M691278), and the Research Project Plan of the Wuxi Municipal Health Commission (Q202243). We would like to thank Prof. Minchen Wu at the Wuxi School of Medicine for the technical assistance.

Conflicts of interest:

The authors have no conflict of interest to declare.

Received: October 9, 2023; Revised: February 5, 2024; Accepted: March 13, 2024; Prepublished online: March 13, 2024; Published: March 15, 2024  Show citation

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Liu Y, Zhang H, Li Y, Zha H, Gao Y, Chen H, et al.. Dictyophora indusiata polysaccharide mediates priming of the NLRP3 inflammasome activation via TLR4/ NF-κB signaling pathway to exert immunostimulatory effects. J Appl Biomed. 2024;22(1):23-32. doi: 10.32725/jab.2024.005. PubMed PMID: 38505967.
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