Journal of Applied Biomedicine, 2024 (vol. 22), issue 1
Original Research Article
Prenatal exposure to polycyclic aromatic hydrocarbons and growth parameters
Radim J. ©rám, Ivo Solanský, Anna Pastorková, Miloą Velemínský, Jr., Miloą Velemínský, Kateřina Hoňková, Hana Baroąová, Jana Schmuczerová, Kateřina Urbancová, Darina Dvořáková, Jana Pulkrabová
J Appl Biomed 22:12-22, 2024 | DOI: 10.32725/jab.2024.004 
Background and objectives: It has long been known that airborne polycyclic aromatic hydrocarbons (PAHs) can negatively affect pregnancy and birth outcomes, such as birth weight, fetal development, and placental growth factors. However, similar studies yield divergent results. Our goal was to estimate the amount of monohydroxylated PAH (OH-PAH) metabolites in the urine of pregnant women/mothers and their newborns in relation to birth outcomes, such as placenta weight, Apgar 5', and the growth parameters of children up to the age of two. Methods: Two cohorts of children born in 2013 and 2014 during the summer and winter seasons in the Czech Republic...
Dictyophora indusiata polysaccharide mediates priming of the NLRP3 inflammasome activation via TLR4/ NF-κB signaling pathway to exert immunostimulatory effects
Youyi Liu, Huanxiao Zhang, Yuxuan Li, Hanqian Zha, Yujie Gao, Hui Chen, Yalin Wang, Tongxin Zhou, Chao Deng
J Appl Biomed 22:23-32, 2024 | DOI: 10.32725/jab.2024.005
Dictyophora indusiata, commonly known as bamboo fungus, is a type of edible mushroom that is highly popular worldwide for its rich flavor and nutritional value. It is also recognized for its pharmaceutical efficacy, with medicinal benefits attributed to its consumption. One of the most important components of Dictyophora indusiata is polysaccharide, which has been acknowledged as a promising regulator of biological response due to its immunostimulatory and anti-inflammatory properties. However, the specific roles of polysaccharide in modulating the NOD-like receptor protein 3 (NLRP3) inflammasome activation within macrophages remain relatively...
Anti-inflammatory effect of luteoloside against methylglyoxal induced human dental pulp cells
Ji-Eun Kim, Pradhan Paras Man, Sungil Jang, Ho-Keun Yi *
J Appl Biomed 22:33-39, 2024 | DOI: 10.32725/jab.2024.002
Purpose: The aim of this study was to investigate whether luteoloside, a flavonoid, could protect human dental pulp cells (HDPCs) against inflammation and oxidative stress induced by methylglyoxal (MGO), one of the advanced glycated end products (AGE) substances. Methods: HDPCs were stimulated with MGO and treated with luteoloside. MTT assay was used to determine cell viability. Protein expression was measured via western blotting. Reactive oxygen species (ROS) were measured with a Muse Cell Analyzer. Alkaline phosphatase activity (ALP) and Alizarin red staining were used for mineralization assay. Results: Luteoloside down-regulated the expression...
Endoplasmic reticulum stress-related protein GRP78 and CHOP levels in synovial fluid correlate with disease progression of primary knee osteoarthritis: A cross-sectional study
Dan-Dan Liu, Yuan-Chuang Zhao, Hai-Hong Li, Lian-Jun Yin, Jun-Qi Chen, Gang Liu
J Appl Biomed 22:40-48, 2024 | DOI: 10.32725/jab.2024.001
Background: Endoplasmic reticulum (ER) stress has been shown to play an important role in osteoarthritis (OA). Objective: This study was aimed at assessing the relationship of endoplasmic reticulum (ER) stress-related glucose-regulated protein 78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP) concentrations in the serum/synovial fluid (SF) with disease severity of primary knee osteoarthritis (pkOA). Methods: Patients with pkOA together with healthy individuals were consecutively recruited from our hospital. The levels of GRP78 and CHOP in serum / SF were detected using enzyme-linked immunosorbent assay. The levels of IL-6 and...
Amelioration of obesity induction by a high-fat diet and related inflammation by Phasa fish (Setipinna phasa) oil in BALB/c mice
Titli Panchali, Ananya Dutta, Pipika Das, Amina Khatun, Riya Kar, Subhadeep Mondal, Keshab Chandra Mondal, Sudipta Chakrabarti, Kuntal Ghosh, Shrabani Pradhan
J Appl Biomed 22:49-58, 2024 | DOI: 10.32725/jab.2024.003
We have extracted and characterized Phasa fish (Setipinna phasa) oil for the first time to evaluate the anti-obesity and related anti-inflammatory effects on obese mice. Inbred male albino BALB/c mice were segregated into three categories: control (C), Obese control group (OC), and Phasa fish oil treated group (TX). To establish the potentiality of Setipinna phasa oil for its anti-obesity and anti-inflammatory properties, it was extracted and characterized using GC-MS method. To evaluate the anti-obesity effect, different parameters were considered, such as body weight, lipid composition, obesity, and obesity associated inflammation....
Rapid triage and transfer system for patients with proven Covid-19 at emergency department
Martin Jakl, Jana Berková, Tomáą Veleta, Vladimír Palička, Petra Polcarová, Jan Smetana, Petr Grenar, Martina Čermáková, Jan Vaněk, Jan M. Horáček, Jaromír Kočí
J Appl Biomed 22:59-65, 2024 | DOI: 10.32725/jab.2024.006
Background: COVID-19 is a viral disease notorious for frequent worldwide outbreaks. It is difficult to control, thereby resulting in overload of the healthcare system. A possible solution to prevent overcrowding is rapid triage of patients, which makes it possible to focus care on the high-risk patients and minimize the impact of crowding on patient prognosis. Methods: The triage algorithm assessed self-sufficiency, oximetry, systolic blood pressure, and the Glasgow coma scale. Compliance with the triage protocol was defined as fulfillment of all protocol steps, including assignment of the correct level of care. Triage was considered successful if...
Review Article
Selected widely prescribed pharmaceuticals: toxicity of the drugs and the products of their photochemical degradation to aquatic organisms
©árka Klementová *, Martina Poncarová
J Appl Biomed 22:1-11, 2024 | DOI: 10.32725/jab.2024.007
Cholesterol-lowering drugs, antidiabetics, antiarrhythmics, antidepressants, and antibiotics belong to the most prescribed drugs worldwide. Because of the manufacture, excretion, and improper disposal of leftover drugs, the drugs enter waste waters and, subsequently, surface waters. They have been detected in surface waters all over the world, from concentrations of ng/l to concentrations several orders of magnitude higher. Since pharmaceuticals are designed to be both biologically and chemically stable, photochemical degradation by sun radiation represents a way of transformation in the natural environment. This review provides a survey of how selected...