Journal of Applied Biomedicine, 2003 (vol. 1), issue 2

To the mystery of cell biology

Karel Smetana

J Appl Biomed 1:51-53, 2003 | DOI: 10.32725/jab.2003.008  

Comparison of the reactions to stress produced by X-rays or electromagnetic fields (50Hz) and heat: induction of heat shock genes and cell cycle effects in human cells

Sergey V. Tokalov, Stefan Pieck, Herwig O. Gutzeit

J Appl Biomed 1:85-93, 2003 | DOI: 10.32725/jab.2003.013  

The effects of irradiation (200kV X-ray, 5 Gy), extremely low frequency electromagnetic fields (ELF-EMF, 50 Hz, 60±0.2 μT) and/or heat shock (HS, 41°C for 30 min) on the expression of several heat shock genes (HSP27, HSP60, HSP70, HSP75, HSP78, HSP90) in human HL-60 leukaemia cells was studied by RT-PCR. In addition, the effects of these stressors on cell cycle progression in exponentially and asynchronously growing cell cultures were studied by flow cytometry. The dynamics of cell division during successive cell cycles was monitored by fluorescence-labelling of the cells with carboxyfluorescein succinimidyl ester (CFSE). Finally, the cell...

Are nucleoli participating in programmed cell death? (MINI-REVIEW AND RECENT OBSERVATIONS)

Karel Smetana

J Appl Biomed 1:93-97, 2003 | DOI: 10.32725/jab.2003.014  

Despite numerous publications on nucleoli, their participation in terminal maturation and programmed cell death have not been studied extensively. On the other hand, some observations clearly indicate that terminal maturation and programmed cell death are accompanied by marked nucleolar changes which reflect the cessation of nucleolar biosynthetic activities. In addition, nucleolar changes in the course of terminal maturation may precede programmed cell death.

Capillary electrophoresis determination of huperzine A: A new prospective anti-dementia drug

Eladia M. Peña-Méndez, Tomáš Hottmar, Josef Havel, Jiří Patočka

J Appl Biomed 1:99-106, 2003 | DOI: 10.32725/jab.2003.015  

Huperzine A is an alkaloid isolated from the Chinese plant Huperzia serrata. The absorption spectra and electrophoretic behaviour of the compound were studied. The compound shows two absorption maxima (λ_max 230 and 310nm). The protonation constant was determined by spectrophotometry and capillary zone electrophoresis (CZE) and the recommended value is pK=7.70±0.07. A CZE method for the determination of Huperzine A in pharmaceutical tablets and serum has been developed. The method is based on separation of the compound as a protonated species with electrophoretic mobility determined as 50.7 x 10^-9 m²V^-1s^-1....

Nuclear terrorism

Pavel Kuna, Otakar Neruda, Leoš Navrátil, Jan Matzner, Renata Žišková

J Appl Biomed 1:55-59, 2003 | DOI: 10.32725/jab.2003.009  

Neutron weapons are designed for the destruction of living organisms; they are not designed for the destruction of building and technical facilities. The miniature nuclear fission weapons (about 1 kt) have similar characteristics and they are most suitable for terrorist attacks. Irradiation of mammals by fission neutrons results in a more severe deterministic (acute) as well as stochastic (late) radiation damage in comparison with gamma rays. Traditional physical shielding materials (steel, lead) cannot provide protection, and biological protection mechanisms (tissue hypoxia, radioprotective chemical drugs) are also not effecive. Water and plastic...

The chemistry, pharmacology, and toxicology of the biologically active constituents of the herb Hypericum perforatum L.

Jiří Patočka

J Appl Biomed 1:61-70, 2003 | DOI: 10.32725/jab.2003.010  

St. John's wort (Hypericum perforatum) has been used as a medical herb for over 2000 years. Over the past two decades, its application as a standardized plant extract for treating depression has undergone rigorous scientific investigation, and its effectiveness has been shown in studies comparing it with other commonly used antidepressants and placebos. Safety and tolerability studies have revealed that Hypericum preparations have better safety and tolerability profiles than synthetic antidepressants. The indications for St. John's wort preparations are mild or moderate depression. The mechanism of the antidepressant action of Hypericum...

Opacity simulation technique for confocal microscope image processing

Miloslav Druckmüller

J Appl Biomed 1:71-75, 2003 | DOI: 10.32725/jab.2003.011  

3D-visualization of small transparent objects like cells by means of classical methods often gives 3D sensation insufficient for the understanding of objects topology. Method introducing artificial opacity are described in this article.

Why do circadian biorhythms age?

Josef Berger

J Appl Biomed 1:77-84, 2003 | DOI: 10.32725/jab.2003.012  

Circadian biorhythms change with age and such changes are caused by the loss of both the time and the space structure. These alterations of biorhythms are associated with poor health and the end of life but we do not know the extent to which they represent cell clock system injury. It seems that ageing of biorhythms in mammals, i.e. including humans, is caused by the ability of suprachiasmatic nuclei to drive oscillations in other tissues. Social synchronization extending photic stimuli, which diminishes during degeneration of nerve and optic system, enhances the quality of life and therefore further studies of the influence of health and social care...

Jubilee of Václav Řehout

Josef Berger, Jindřich Čítek

J Appl Biomed 12003  

Cooperation with the South African Police Service

Vladimír Vurm

J Appl Biomed 1:110, 2003  

The practical HRQOL assessement

Josef Dolista, Paul H. Möller

J Appl Biomed 1:110-111, 2003  

A system supporting electronic education

Michal Šerý, Milan Novák

J Appl Biomed 1:111, 2003  

Laser Florence 2002

Leoš Navrátil

J Appl Biomed 1:107-108, 2003  

Pharmacological days in Marseille 2002

Josef Berger

J Appl Biomed 1:107, 2003  

The joint EMBO/EU practical course on electron microscopy and stereology

Jana Nebesářová, Ivan Raška

J Appl Biomed 1:108, 2003  

5th conference on cell biology

Josef Berger

J Appl Biomed 1:108-109, 2003  

11th International Vasculitis and ANCA Workshop

Jiřina Bartůňková

J Appl Biomed 1:109, 2003