Journal of Applied Biomedicine, 2005 (vol. 3), issue 2
Original Research Article
Phagocytes may counteract the "open window" situation during a bout of moderate exercise performed by sedentary individuals: role of noradrenaline
Eduardo Ortega, Juan José García, Jose Maria Marchena, Carmen Barriga, Ana Beatriz Rodríguez
J Appl Biomed 3:75-82, 2005 | DOI: 10.32725/jab.2005.009 
Changes in the blood number of peripheral lymphocytes, monocytes and granulocytes at different times (basal, at 7 min, and immediately after exercise) during moderate exercise (55% VO2 max; 60 min) performed by sedentary men were investigated. These variations were also evaluated during a recovery period (45 min and 24 h after finishing the exercise), and were correlated with changes in catecholamines and cortisol. The T (total CD3+, CD4+, and CD8+) and B (CD19+) lymphocytes showed similar behaviour during exercise, although the changes were more marked in T cells: an increase 7 min after the start...
Synthesis and activity of 4-(2',4'-difluorobiphenylyl)-2-methylbutyric acid (deoxoflobufen) and its derivatives
Petr Bulej, Miroslav Kuchař, Vladimíra Panajotova, Alexandr Jegorov
J Appl Biomed 3:83-90, 2005 | DOI: 10.32725/jab.2005.010
The series of 4-(2',4'-difluorobiphenylyl)-2-methylbutyric acid (deoxoflobufen, 1) and its four amides and two salts were prepared and tested for anti-inflammatory activity in rats and mice, using as models carrageenan-induced paw oedema, pleuritis, and arachidonic acid-induced ear inflammation, and on leucotriene B4 production in cells.
Tea tree oil inhalations modify immunity in mice
Mateusz Golab, Oleg Burdzenia, Pawel Majewski, Krystyna Skwarlo-Sonta
J Appl Biomed 3:101-108, 2005 | DOI: 10.32725/jab.2005.012
Tea tree oil (TTO) is well known as an anti-microbial and immunomodulatory agent and the latter property was examined in this study. Male, C57BI10 x CBA/H (F1), mice were exposed to TTO vapour three times a day, for one week. During this period, half of the mice also received naltrexone (endogenous opioid receptor antagonist) in their drinking water. A day before the end of the TTO inhalation treatment a number of the mice were intra-peritoneally injected with Zymosan or PBS. Spleens and peritoneal exudates were collected 24 h after the injections. Cultured splenocytes were used in in vitro proliferation assays with PHA,...
Reviews
Selenium and the prostate
Wilfred N. Arnold
J Appl Biomed 3:59-66, 2005 | DOI: 10.32725/jab.2005.007
Evidence accrued over the last decade has supported an association between low serum selenium (Se) and increased incidence of prostate cancer in older men. Accordingly, questions and inquiry arose as to whether a dietary supplementation might afford protection, or delay disease progression. Indeed, a very large national study, Selenium and Vitamin E Cancer Prevention Trial (SELECT), to explore the potential benefits of a daily supplement of 200 microgram Se, or 400 I.U. of vitamin E, or both, is underway: the National Cancer Institute anticipates a total enrolment of 32,400 men and durations of 7 to 12 years for individual participants. It is remarkable...
Neuroprotective peptides as drug candidates against Alzheimer's diasease
Jiří Patočka, Jiřina Slaninová, Gabriela Kunešová
J Appl Biomed 3:67-73, 2005 | DOI: 10.32725/jab.2005.008
First described by Alois Alzheimer in 1907, Alzheimer's disease (AD) is the most common dementia type, affecting approximately 20 million people worldwide. As the population is getting older, AD is a growing health problem. AD is characterized by the presence of neuritic plaques containing the amyloid-beta peptide (Abeta) and an intraneuronal accumulation of tubule-associated protein called tau. Currently available treatment used in AD is based on acetylcholinesterase inhibitors, since in the course of AD there is a substantial loss in cholinergic neurons. Another registered drug used in more severe AD is the NMDA antagonist-memantine. From a drug...
Oxime reactivation of acetylcholinesterase inhibited by toxic phosphorus esters: in vitro kinetics and thermodynamics
Jiří Patočka, Jiří Cabal, Kamil Kuča, Daniel Jun
J Appl Biomed 3:91-99, 2005 | DOI: 10.32725/jab.2005.011
Owing to the threat of organophosphate exposures, not only to pesticides but also to nerve agents, it is very important to know the whole process of organophosphates-inhibited acetylcholinesterase (AChE, EC 3.1.1.7) reactivation. Although current antidotes against organophosphorus intoxications consist also of prophylactics, AChE reactivators are still needed especially in the case of intoxications with high doses of organophosphates, for which prophylactic treatment is not effective. For this reason, new AChE reactivators are still being developed. Our work summarizes accurately the whole reactivation process, and offers some help for scientists who...