Journal of Applied Biomedicine, 2005 (vol. 3), issue 3
Original Research Article
Nycthemeral change of some haematological parameters in horses
Giuseppe Piccione, Francesco Fazio, Elisabetta Giudice, Fortunata Grasso, Massimo Morgante
J Appl Biomed 3:123-128, 2005 | DOI: 10.32725/jab.2005.015 
Many physiological processes of domestic animals exhibit daily rhythmicity. The goal of the present study was to investigate, in horses, the existence of the daily rhythms of some haematological parameters: red blood cells (RBC), white blood cells (WBC), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and platelets (PLT). Blood samples from 6 Thoroughbred mares were collected at 4-hour intervals for 48 hours (starting at 08:00 hours on day 1 and finishing at 04:00 on day 2) via an intravenous cannula inserted into the jugular vein. ANOVA showed...
Population genetics of 11 nuclear and 1 mitochondrial short tandem repeat loci in a population of South Bohemia, Czech Republic
Tomáš Vaněček, Pavel Trubač, František Vorel, Miroslav Šíp
J Appl Biomed 3:129-131, 2005 | DOI: 10.32725/jab.2005.016
A population study on 11 nuclear and 1 mitochondrial short tandem repeats (STR) (CSF1PO, TPOX, THO1, F13A01, FESFPS, vWA, D16S539, D7S820, D13S317, LPL, F13B and mitochondrial (CA)n repeat (mtCA)) was performed, and allele frequencies together with other forensic genetics characteristics, were estimated from a sample of 111 - 253 unrelated individuals living in the area of South Bohemia, Czech Republic. All loci, except F13B, meet Hardy-Weinberg expectations. Significant differences of allele frequencies were found in three (vWA, D16S539 and F13B) or two loci (vWA, D16S539) when compared with reference or known Czech data, respectively....
Protective influence of Pongamia pinnata (Karanja) on blood ammonia and urea levels in ammonium chloride-induced hyperammonemia: antihyperammonemic effect of the leaf extract
Musthafa Mohamed Essa, Perumal Subramanian, Ganapathy Suthakar, Tamilarasan Manivasagam, Kadiyala Babu Dakshayani
J Appl Biomed 3:133-138, 2005 | DOI: 10.32725/jab.2005.017
The present study was undertaken to investigate the antihyperammonemic efficacy of the leaf extract of Pongamia pinnata, an indigenous plant used in Ayurvedic Medicine in India (PPEt), on blood ammonia, plasma urea, uric acid, non-protein nitrogen and serum creatinine in control and ammonium chloride induced hyperammonemic rats. The levels of blood ammonia, circulatory urea, uric acid, non-protein nitrogen and creatinine increased significantly in rats treated with ammonium chloride and decreased significantly in rats treated with PPEt and ammonium chloride. There were no significant changes in the body weights of the experimental animals when...
Prediction of a new broad-spectrum reactivator capable of reactivating acetylcholinesterase inhibited by nerve agents
Vlastimil Dohnal, Kamil Kuča, Daniel Jun
J Appl Biomed 3:139-145, 2005 | DOI: 10.32725/jab.2005.018
A methodology combining molecular structure represented by fragments, and artificial neural network (ANN) was applied for the prediction of a new acetylcholinesterase (AChE; EC 3.1.1.7) reactivator. We searched for a new structure of the AChE reactivator with the capability of reactivating AChE inhibited by almost all actual nerve agents. For this purpose, we have tested in vitro seventeen potential AChE reactivators for reactivation of AChE inhibited by sarin, cyclosarin, agent VX and tabun. The results obtained were used as input data for prediction by ANN. Using ANN we have predicted new AChE reactivators.
Reviews
Current ethical problems in cell biology
Josef Berger
J Appl Biomed 3:109-113, 2005 | DOI: 10.32725/jab.2005.013
We selected important ethical conflicts of interest in current cell biology and which remain still unresolved: the use of human biological material, patents for biological material, cloning and use of stem cells, genetic engineering, onset of human life and death, and the use of vertebrate biomodels. New data from cell and molecular biology cannot facilitate the solution of such problems - on the contrary, they make these solutions more difficult. A solution may be found within the socio-cultural problems. Intensive research is necessary both to accomplish economical and medical benefit and to clarify ethical rules. Thus, essential cell biology seems...
Inhibitors of histone-deacetylase
Jiřina Vávrová, Martina Řezáčová, Jan Osterreicher
J Appl Biomed 3:115-121, 2005 | DOI: 10.32725/jab.2005.014
Histone acetyltransferase and histone deacetylase activities determine the acetylation status of histones, and have the ability to regulate gene expression through chromatin remodeling. A controlled balance between histone acetylation and histone deacetylation appears to be essential for normal cell growth. In cancer cells, some genes are repressed by inappropriate recruitment of histone deacetylases. The histone deacetylase inhibitors (HDACI) belong to the class of anticancer drugs that are effective in killing proliferating and non-proliferating tumor cells. In this review we discuss molecular mechanisms involved in the induction of cell cycle arrest,...
Brief Communications
Positron emission tomography (PET) in residual post-treatment Hodgkin's disease masses
Gustavo H. Marin, Jorge Dellagiovanna, Pablo Urdampilleta
J Appl Biomed 3:147-153, 2005 | DOI: 10.32725/jab.2005.019
Hodgkin's Disease (HD) is one of the malignant diseases with good chances for a cure. The prognosis for cure depends on the initial stages and the outcome after initial treatment. Stages I or II at diagnosis, or a complete remission after initial treatment are good indicators for the best prognosis to patients.A frequent problem faced by clinicians is met at the post-therapy stage because of the difficulty of distinguishing between dead tissue and disease activity in residual masses. Considering that these two situations include therapy options that run from treatment abstention to autologous stem cell transplantation, it is extremely important...