Journal of Applied Biomedicine, 2012 (vol. 10), issue 1
Original Research Article
Influence of circulating fibrocytes on the growth, proliferation and migration of keratinocytes and fibroblasts
Peng Liu, Gang Hu, Jie Feng, Yan Jin
J Appl Biomed 10:9-17, 2012 | DOI: 10.2478/v10136-011-0005-4 
Circulating fibrocytes (CFs) exhibit an extraordinary degree of plasticity and growth factor repertoire, and because of this they have been investigated for their role in the repair and regeneration of damaged tissues, but yet not adequately for their role in wound healing. In the present study, CFs were co-cultured with keratinocytes (KCs) or fibroblasts (Fbs) and the influences of CFs on the growth, proliferation and migration of KCs and Fbs were investigated. Our results showed that the CFs in the co-culture system could inhibit the growth, proliferation and migration of KCs, while CFs promoted the growth and proliferation of Fbs. Our study demonstrates...
Variant within CELSR2/PSRC1/SORT1, but not within CILP2/PBX4, PCSK9 and APOB genes, has a potential to influence statin treatment efficacy
Jaroslav Alois Hubáček, Věra Adámková, Věra Lánská, Dana Dlouhá, Jitka Rynekrová, Lukáš Zlatohlávek, Martina Prusíková, Richard Češka, Michal Vrablík
J Appl Biomed 10:19-28, 2012 | DOI: 10.2478/v10136-012-0001-3
Statins have become a cornerstone of cardiovascular prevention. However, their lipid lowering efficacy and, thus also, impact on event risk reduction, differ substantially between individuals. The major part of this inter-individual difference can be explained by genetic factors. Using the GWA approach, candidate genes that may modify the response to statin treatment have been detected. Variants rs646776 (CELSR2/PSRC1/SORT1), rs16996148 (CILP2/PBX4), rs11206510 (PCSK9) and rs693 (APOB) were analysed in 370 (146 males) dyslipidemic patients treated with statins (46.6% simvastatin, 41.5% atorvastatin, 11.9% lovastatin, 10...
The role of the light/dark cycle in the daily rhythm of serum proteins in Equus caballus
Giuseppe Piccione, Simona Marafioti, Claudia Giannetto, Caterina Faggio, Michele Panzera, Francesco Fazio
J Appl Biomed 10:29-34, 2012 | DOI: 10.2478/v10136-011-0019-y
This research was carried out on five clinically healthy Sella Italiana horses to determine the daily rhythm of total proteins and their fractions, to establish if these rhythms are endogenously generated and to assess the role of light as synchroniser of these rhythms. Blood samples were collected from each subject every 3 h over a period of 48 h, starting at 9:00 on day 1 and finishing at 9:00 on day 3, into vacutainer tubes without an anticoagulant via intravenous cannulas inserted into the jugular vein. Total serum proteins, albumin, α1-, α2-, β1-, β2- and γ-globulin concentrations...
Phagocytosis of insect haemocytes as a new alternative model
Josef Berger, Martina Jurčová
J Appl Biomed 10:35-40, 2012 | DOI: 10.2478/v10136-012-0003-1
Phagocytosis is an important function of both insect haemocytes and mammalian blood cells. Linden bugs and cotton leaf worms have been suggested as new alternative models for ecological and drug toxicology but no data on their haemocyte physiology have been published. Our assays with particle ingestion of the NBT test were carried out on prohaemocytes, granulocytes, plasmatocytes and spherulocytes of adult linden bug and cotton leaf worm larvae. We found that phagocytic activity is on average 10% in the linden bug, and 50% in cotton leaf worm haemocytes: the phagocytic index is 3.5 in both species and nitroblue tetrazolium reduction is 0.5 in the linden...
Jerte Valley cherry-based product modulates serum inflammatory markers in rats and ringdoves
Jonathan Delgado, María del Pilar Terrón, María Garrido, Carmen Barriga, Javier Espino, Sergio Damián Paredes, Ana Beatriz Rodríguez
J Appl Biomed 10:41-50, 2012 | DOI: 10.2478/v10136-011-0009-0
Ageing is commonly accompanied by a chronic subclinical inflammatory status that coexists with immune dysfunction. Consumption of foods rich in antioxidants is associated with a lower incidence of chronic diseases. The aim of this study was to evaluate the effect of the consumption of a Jerte Valley cherry-based beverage on the inflammatory load in two different animal species: rats and ringdoves (Streptopelia risoria); each divided into two age groups. To this purpose, circulating levels of both pro-inflammatory (IL-1β and TNF-α) and anti-inflammatory (IL-4 and IL-2) cytokines were measured before and after a 10-day treatment with...
Soman and VX: different effect on cellular signalling
Jaroslav Pejchal, Jan Österreicher, Jiří Kassa, Aleš Tichý, Zuzana Šinkorová, Lenka Zárybnická, Klára Kubelková, Kamil Kuča
J Appl Biomed 10:51-61, 2012 | DOI: 10.2478/v10136-011-0018-z
The purpose of our study was to examine the early expression of p21 and activated transcription factors ATF-2, CREB, Elk-1, p53 after soman and VX poisoning, to throw light on the pathogenetic mechanism of nerve agent-induced non-specific effects. Male Wistar rats were i.m. poisoned by soman (60 μg.kg-1 - 70% LD50) or VX (8 μg.kg-1 - 70% LD50). Samples were taken 4, 24, and 72 hours after poisoning, immunohistochemically stained and phospho-ATF-2Thr-69/71, phospho-CREBSer-133, phospho-Elk-1Ser-383, phospho-p53Ser-15, and protein p21 expressions were measured...
Reviews
Fullerene nanoparticles and their anti-oxidative effects: a comparison to other radioprotective agents
Jiřina Vávrová, Martina Řezáčová, Jaroslav Pejchal
J Appl Biomed 10:1-8, 2012 | DOI: 10.2478/v10136-012-0002-2
Radiation therapy occupies an important position in the treatment of malignant diseases in spite of the existence of radiation side effects on normal tissues. Thus, substances are being developed which are designed to reduce both the acute and long term radiation effects on healthy tissues. Currently a sulphur-containing compound amifostine (WR2721, ethyol) is used in clinical practice as a radioprotectant. However, it itself has considerable side effects including hypotension (found in 62% of patients), hypocalcaemia, diarrhoea, nausea, and vomiting. Carbon nanospheres, known as fullerenes, and their water soluble derivatives (e.g. C60(OH)24,...