Journal of Applied Biomedicine, 2012 (vol. 10), issue 4
Original Research Article
Expression of Mycobacterium tuberculosis proteins MPT63 and MPT83 as a fusion: purification, refolding and immunological characterization
Taras Redchuk, Natalia Korotkevich, Oksana Gorbatiuk, Pavlo Gilchuk, Andrii Kaberniuk, Olena Oliynyk, Denis Kolibo, Serhiy Komisarenko
J Appl Biomed 10:169-176, 2012 | DOI: 10.2478/v10136-012-0013-z 
Proteins MPT63 and MPT83 which are common for both Mycobacterium tuberculosis and Mycobacterium bovis, due to their high immunogenicity, are thought to play a promising role in the development of immunodiagnostic reagents and vaccines. To enhance the antigenic and immunogenic properties of these proteins, fragments of the mpt83 and mpt63 genes were fused in tandem. In this article we present an effective method for the MPT63-MPT83 fusion product purification by metal-affinity chromatography and in vitro refolding. Our results demonstrate that the antigenic properties of the recombinant proteins obtained are comparable...
Cilostazol suppresses LPS-stimulated maturation of DC2.4 cells through inhibition of NF-κB pathway
Tianhua Chen, Zhiliang Li, Qiang Fu, Linlin Chen, Longxing Cao, Weiwei Zhang, Jianxin Diao
J Appl Biomed 10:177-186, 2012 | DOI: 10.2478/v10136-012-0012-0
Cilostazol is a phosphodiesterase-3 inhibitor that functions as a platelet aggregation inhibitor and is used for treating peripheral artery diseases and ischemic stroke. Dendritic cells (DCs) play an active role in the immunological processes related to atherosclerosis. Cilostazol has anti-atherogenic and anti-inflammatory effects, but the effects of cilostazol on DC maturation remain unknown. The purpose of this study was to determine the effects of cilostazol on lipopolysaccharide (LPS)-induced maturation of DCs. DC2.4 cells were treated with cilostazol for 12 h and subsequently stimulated with LPS to induce maturation. Cilostazol reduced the expression...
Acute poisoning with sarin causes alteration in oxidative homeostasis and biochemical markers in Wistar rats
Miroslav Pohanka, Jaroslav Románek, Jiří Pikula
J Appl Biomed 10:187-193, 2012 | DOI: 10.2478/v10136-012-0010-2
Sarin is a potent inhibitor of acetylcholinesterase (AChE). It is known as an agent of chemical warfare and is one of a number of nerve agents misused for chemical terrorism, e.g. on the Tokyo subway attacks. Though effect of sarin on the cholinergic system is well-known, long-term adverse effects and the role of oxidative stress in sarin toxicity remain unknown. The experiment reported here was carried out on laboratory Wistar rats intramuscularly exposed to 0.5-50% of sarin LD50 for one hour. A complex biochemical examination of plasma samples and an assessment of oxidative stress in the liver, kidney, spleen, cerebellum and frontal lobe...
Antioxidative/oxidative effects of strontium-doped bioactive glass as bone graft. In vivo assays in ovariectomised rats
Samira Jebahi, Hassane Oudadesse, Hafed el Feki, Tarek Rebai, Hassib Keskes, Pascal Pellen, Abdelfattah el Feki
J Appl Biomed 10:195-209, 2012 | DOI: 10.2478/v10136-012-0009-8
Recently, oxidative stress has been identified as a pivotal pathological factor inducing bone osteoporosis. This phenomenon is responsible for low bone density. It alters bone quality and generates bone fractures. Strontium is found to induce osteoblast activity by stimulating bone formation and reducing bone resorption by restraining osteoclasts. Bioglass (BG) has been used to repair bone defects, and, in combination with strontium (BG-Sr), offers an opportunity to treat this disease. This study investigated the potential role of BG-Sr in improving antioxidant activity and regenerative bone capacity, The effects of both BG-Sr and BG were tested on...