Journal of Applied Biomedicine, 2013 (vol. 11), issue 4

Relationship between serum bilirubin and uric acid to oxidative stress markers in Italian and Czech populations

Libor Vítek, Ladislav Novotný, Aleš Žák, Barbora Staňková, Tomáš Zima, Angela Polito, Greco Cesare, Chiara Zerbinati, Luigi Iuliano

J Appl Biomed 11:209-221, 2013 | DOI: 10.2478/v10136-012-0030-y  

Recently, a South-to-North oxidative stress marker gradient has been reported; consistent with known differences in the incidence of coronary heart disease between southern and northern European countries. The aim of the present study was to compare the plasma concentrations of 7-oxocholesterol (7OxCH) and 7β-hydroxycholesterol (7BCH) with systemic antioxidants in healthy Italian and Czech subjects. The study was performed in healthy subjects of Italian (n=131) and Czech (n=84) origins. In all subjects routine biochemistry work-ups were performed; additionally, plasma oxysterols and the peroxyl radicals scavenging activity (PERSA) of the sera...

Nitrite contamination in hypotensive preparations of dinitrosyl iron complexes with glutathione

Rostislav Romanovich Borodulin, Vyacheslav Olegovich Shvydkiy, Lyudmila Nikolaevna Kubrina, Vasak Dzhanibekovich Mikoyan, Natalya Ilinishna Zaitseva, Tatyana Evgenjevna Sporyagina, Sergey Alexandrovich Kryuchkov, Anatoly Fjodorovich Vanin

J Appl Biomed 11:223-233, 2013 | DOI: 10.2478/v10136-012-0025-8  

The content of nitrite admixture in preparations of dinitrosyl iron complexes (DNIC) with glutathione synthesized by treatment of aqueous solutions of Fe²⁺ + glutathione with gaseous NO (complex 1) or by mixing solutions of S-nitrosoglutathione (GS-NO) with solutions of Fe²⁺ + glutathione (complex 2) was determined using the Griess method and HPLC as well as from the level of HNO₂ formed upon interaction of gaseous NO with acidified distilled water. In both preparations, DNIC were predominantly represented by the binuclear form (B-DNIC). In complex 1, the appearance of nitrite in DNIC solutions was induced by nitrogen...

Effects of therapeutic ultrasound on the nuclear envelope and nuclear pore complexes

Naděžda Vaškovicová, Zdena Druckmüllerová, Roman Janisch, Jiřina Škorpíková, Vojtěch Mornstein

J Appl Biomed 11:235-242, 2013 | DOI: 10.2478/v10136-012-0042-7  

The effects of acoustic waves on membrane structures, and any resulting consequences of this treatment on membrane subunit structures, remain poorly understood, as are the principals of related clinical effects. With a focus on morphological changes in the nuclear envelope, the current study presents detailed observations of membrane structures exposed to therapeutic ultrasound. Ultrasound treatment most commonly resulted in distinct changes in the distribution of nuclear pore complexes (NPCs) and mean NPC number per unit area after 30 min of repair, as well as alterations in NPC diameters on the protoplasmic face of fractured nuclear membranes after...

The heterochromatin condensation state in central nuclear regions of individual granulocytes

Karel Smetana, Dana Mikulenková, Hana Klamová

J Appl Biomed 11:243-249, 2013 | DOI: 10.2478/v10136-012-0041-8  

The knowledge on the heterochromatin condensation state (HChCS) in central nuclear "gene rich" regions is limited although the heterochromatin reflects the gene silencing. Therefore, the HChCS in these regions was studied in proliferating granulocytic progenitors and terminally differentiated (mature) neutrophilic granulocytes. The HChCS was measured using computer assisted image optical densitometry at the single cell level. The bone marrow smears of patients with chronic myeloid leukemia represented a convenient model because of the increased number of progenitor and mature cells for density measurements. In addition, the heterochromatin density...

In memoriam Prof. Franz Halberg (1919-2013)

Germaine Cornelissen, Francine Halberg, Julia Halberg, Othild Schwartzkopff

J Appl Biomed 11:251, 2013 | DOI: 10.1016/S1214-021X(14)60067-3