Journal of Applied Biomedicine, 2015 (vol. 13), issue 4

Contents

J Appl Biomed 13:v, 2015 | DOI: 10.1016/S1214-021X(15)00057-5  

Depletion of androgen receptor (AR) in mesenchymal stem cells (MSCs) inhibits induction of CD4+CD25+FOX3+ regulatory T (Treg) cells via androgen TGF-β interaction

Abdullah Alawad, Saleh Altuwaijri, Ahmed Aljarbu, Ilona Kryczek, Yuanjie Niu, Fahd A. Al-sobayil, Chawnshang Chang, Ali Bayoumi, Weiping Zou, Volker Rudat, Mohamed Hammad

J Appl Biomed 13:263-271, 2015 | DOI: 10.1016/j.jab.2015.06.002  

MSCs produce CD4(+)CD25(+)FOX3(+) regulatory T (Treg) cells from activated peripheral blood mononuclear cells (PBMC), T-CD4+ and T-CD8+ cells in vitro and in vivo. Here we investigated whether the deficiency of androgen/AR in MSCs influence Treg induction from total PBMC, splenocytes, CD4+CD25-through AR/TGF-β interaction. Eight to 12-week-old wild type and general androgen receptor knockout (ARKO) mice were used. MSCs were collected, characterized and function of Treg cells was studied. Our result showed that depletion of AR suppressed the immunosuppressive effect of MSCs, and demonstrated that WT-MSC-induced Treg cell expansion...

The effect of different doses of atropine on gastric myoelectrical activity in fasting experimental pigs

Jan Bures, Jaroslav Kvetina, Ilja Tacheci, Michal Pavlik, Martin Kunes, Stanislav Rejchrt, Kamil Kuca, Marcela Kopacova

J Appl Biomed 13:273-277, 2015 | DOI: 10.1016/j.jab.2015.04.004  

Electrogastrography (EGG) is a non-invasive method for the assessment of gastric myoelectrical activity. Porcine EGG is comparable with human one. There are no data on the impact of moderate to high doses of atropine on EGG, neither in humans nor experimental pigs. The purpose of this study was to evaluate the effect of different doses of atropine on EGG in experimental pigs.Six fasting pigs entered the study three times in a random order. The baseline EGG recording lasted 20 min, followed by a 105-min EGG trial recording. Intramuscular atropine 1.5 mg (part 1), 3.0 mg (part 2) and 4.5 mg (part 3) was administrated after the baseline EGG.Atropine...

Radiosensitizing potential of Plumbagin in B16F1 melanoma tumor cells through mitochondrial mediated programmed cell death

Bola Sadashiva Satish Rao, Mandala Rayabandla Sunil Kumar, Shubhankar Das, Kiran Aithal, Nayanabhirama Udupa

J Appl Biomed 13:279-288, 2015 | DOI: 10.1016/j.jab.2015.07.003  

The radiosensitizing potential of Plumbagin (PLB) against chemo- and radioresistant B16F1 melanoma cells growing in vitro was investigated. Clonogenic assay revealed a sensitization enhancement ratio (SER) of 1.5 for PLB treatment in combination with radiation. PLB pretreatment for 1 h prior to radiation resulted in elevated intracellular ROS levels compared to the group treated with radiation alone. Alkaline comet assay analysis revealed PLB's potential to enhance the radiation induced DNA damage. Cell cycle studies have shown enhanced G₂/M arrest for combination treatment of PLB with radiation. Cell death exerted by PLB combination...

Serum ferritin levels may have a pro-atherosclerotic role in coronary artery disease patients with sleep disordered breathing

Fernanda S. Hackenhaar, Denis Martinez, Cristini Klein, Tássia M. Medeiros, Paulo V.G. Alabarse, Marco V. Wainstein, Sandro C. Gonçalves, Mara S. Benfato

J Appl Biomed 13:289-298, 2015 | DOI: 10.1016/j.jab.2015.03.006  

Elevated ferritin levels may lead to oxidative stress, and are associated with coronary artery disease (CAD). Sleep disordered breathing (SDB) is frequently present in atherosclerosis patients, and causes endothelial dysfunction leading to atherosclerotic plaque progression. Hypoxic conditions, such as SDB, may upregulate ferritin. The aim of this study was to evaluate ferritin levels in CAD patients and to correlate ferritin levels with parameters related to CAD progression, including SDB. We studied 27 patients with CAD (defined as >30% coronary narrowing) and 29 controls. We found that ferritin was increased in CAD patients, and was positively...

A comparison of the reactivating and therapeutic efficacy of two novel bispyridinium oximes (K920, K923) with the oxime K203 and trimedoxime in tabun-poisoned rats and mice

Jiri Kassa, Vendula Sepsova, Anna Horova, Kamil Musilek

J Appl Biomed 13:299-304, 2015 | DOI: 10.1016/j.jab.2015.07.002  

The potency of two novel oximes (K920, K923) to reactivate tabun-inhibited acetylcholinesterase and to reduce acute toxicity of tabun was compared with the oxime K203 and trimedoxime using in vivo methods. The study determining percentage of reactivation of tabun-inhibited peripheral acetylcholinesterase (diaphragm) and central acetylcholinesterase (brain) in tabun-poisoned rats showed that the reactivating efficacy of both newly developed oximes is lower than the reactivating potency of the oxime K203 and trimedoxime. The therapeutic efficacy of both newly developed oximes roughly corresponds to their weak reactivating efficacy. Their potency...

Lead compound bearing caffeic scaffold induces EGFR suppression in solid tumor cancer cells

Chawannuch Mudjupa, Sherif Abdelhamed, Alaa Refaat, Satoru Yokoyama, Ikuo Saiki, Opa Vajragupta

J Appl Biomed 13:305-317, 2015 | DOI: 10.1016/j.jab.2015.05.001  

A small molecule EGFR inhibitor, 4-(2-(3-(4-(4-(trifluoromethyl)phenyl)thiazol-2-yl)ureido)vinyl)-1,2-phenylene diacetate ( CIU1) was designed in silico by using caffeic scaffold as core structure. The designed compound showed anti-proliferative action against different solid tumor cell lines, particularly metastatic breast cancer cells. CIU1 inhibited the growth of EGFR-overexpressing MDA-MB-468 triple-negative breast cancer cells and wild-type non-small-cell lung cancer H460 cells with IC₅₀ values of 8.96 μM and 12.98 μM, respectively, these anti-proliferative effects of CIU1 were comparable to gefitinib...

Alzheimer's disease: Potential preventive, non-invasive, intervention strategies in lowering the risk of cognitive decline - A review study

Blanka Klimova, Kamil Kuca

J Appl Biomed 13:257-261, 2015 | DOI: 10.1016/j.jab.2015.07.004  

The number of elderly people nowadays is rapidly increasing, especially in the developed countries. This brings about serious problems such as occurrence of diseases connected with aging population. The most frequent aging disease seems to be Alzheimer's disease (AD). The purpose of this article is to briefly describe the individual phases of this disease with special focus on the preclinical stage of AD since in this phase people might be already affected by some brain changes but they are still mentally and physically healthy individuals. Thus, the main goal of this article is to explore the preventive, non-invasive, intervention strategies which...