Journal of Applied Biomedicine, 2016 (vol. 14), issue 1

Contents

J Appl Biomed 14:v, 2016 | DOI: 10.1016/S1214-021X(15)00079-4  

Reviewers Acknowledgements

J Appl Biomed 14:I-II, 2016 | DOI: 10.1016/S1214-021X(15)00080-0

Modulation of ionizing radiation-induced effects by NU7441, KU55933 and VE821 in peripheral blood lymphocytes

Adela Kmochova, Ales Tichy, Lenka Zarybnicka, Zuzana Sinkorova, Jirina Vavrova, Vit Rehacek, Kamila Durisova, Klara Kubelkova, Jaroslav Pejchal, Kamil Kuca

J Appl Biomed 14:19-24, 2016 | DOI: 10.1016/j.jab.2015.07.001  

We evaluated the impact of ataxia-telangiectasia mutated kinase inhibitor KU55933, DNA-dependent protein kinase inhibitor NU7441 and ataxia telangiectasia and rad3-related kinase inhibitor VE821 in human peripheral lymphocytes in vitro. The lymphocytes were divided into 5 groups: non-irradiated control, irradiated group (2 Gy) and 3 groups pretreated with inhibitors 30 min before irradiation. We used flow cytometry to evaluate phosphorylated H2AX (γ-H2AX) and cytotoxicity (Apoptest). Micronucleus assay was used to assess genotoxicity. After irradiation, γ-H2AX, incidence of micronuclei (MN), nucleoplasmatic bridges (NPBs) and nuclear...

Atorvastatin up-regulates the expression and activity of renal Cytochrome P450 3A2 in diabetic rats

Hassan Malekinejad, Shahin Alizadeh-Fanalou, Rahim Hobbenaghi, Shirin Rokhsartalb-Azar

J Appl Biomed 14:25-34, 2016 | DOI: 10.1016/j.jab.2015.08.001  

Effects of atorvastatin on the expression of Cytochrome P450 3A2 and its enzymatic activity in the kidney of diabetic rats were investigated. Diabetes was induced by injection of streptozotocine (50 mg/kg, b.w., i.p.) in male Wistar rats. The animals were assigned into four groups including control (C), non-treated diabetic (D), atorvastatin-treated diabetic (AD) and atorvastatin-treated non-diabetic (A) groups. Metabolism of testosterone was examined in the presence of renal microsomes. The expression of CYP 3A2 at mRNA level was examined by means of PCR technique. The atorvastatin administration resulted in a remarkable improvement of diabetes-induced...

Can we improve clinical results of tonsillectomy using lasers?

David Slouka, Pavel Polenik, Ondrej Hes, David Smid, Karel Slama, Petr Hosek, Ludmila Boudova

J Appl Biomed 14:35-40, 2016 | DOI: 10.1016/j.jab.2015.08.002  

Current medicine uses a variety of high-tech devices to obtain maximum results with minimally invasive procedures. Our goal was to determine the benefits of laser medicine in tonsillectomy in comparison with traditional tonsillectomy, harmonic scalpel and radio frequency scalpel. Forty adult patients with chronic tonsillitis, scheduled for bilateral tonsillectomy, were divided into four groups in a prospective study. The left side tonsillectomy was performed using a traditional technique. The right side tonsillectomy was performed using four different methods: Ho:YAG laser, Er,Cr:YSGG laser, radiofrequency scalpel and harmonic scalpel. Peroperative...

Pharmacological study of a cannabinoid-containing eyedrop formulation in dogs and mice

Aline Maria Stolf, Daiana Lugarini, Anabel de Oliveira, Adriana Pastre Sereniki, Caroline Santos Capitelli, Douglas Hideki Tanaka, Fabiano Montiani Ferreira, Maria Aparecida Barbato Frazão Vital, Alexandra Acco

J Appl Biomed 14:41-48, 2016 | DOI: 10.1016/j.jab.2015.04.005  

Cannabinoids have been indicated for the treatment of glaucoma in humans. However, pharmacological studies in other species are lacking. Healthy Beagle dogs were treated with 0.1% cannabinoid eyedrops for 3 or 7 days. Intraocular pressure (IOP) and pupillary diameter (PD) were measured. To evaluate whether the topical cannabinoid formulation affects the motor and central nervous systems, a parallel study was performed. Male Swiss mice received ocular or intraperitoneal (i.p.) cannabinoid solution for 1 day (acute) or 7 days (subacute) and were tested in the open field (OF), elevated plus maze (EPM), open field habituation test (HT), and marble burying...

Cetrorelix and Triptorelin active immunization influences follicle development and receptor expressions of ovaries in mice

Wei Suocheng, Gong Zhuandi, An Lifeng, Zhang Taojie, Zhang Fengwei, Chen Shien

J Appl Biomed 14:49-57, 2016 | DOI: 10.1016/j.jab.2015.06.003  

The study investigated comparatively the effects of active immunization of Cetrorelix and Triptorelin on the follicle development, histological structures, and expressions of FSHR and LHR proteins in the ovary. One hundred and five female mice, 35 days old, and body weight of 26.56 ± 2.89 g, were randomly assigned into Cetrorelix (CET), Triptorelin (TRI) and control groups (CG). Mice in CET-1, CET-2 and CET-3 (n = 15) were subcutaneously injected with 0.1, 0.2 and 0.4 mL Cetrorelix antigen (100 μg/mL) for seven days, respectively. Mice in TRI-1, TRI-2 and TRI-3 were injected with 0.1, 0.2 and 0.4 mL Triptorelin antigen (100 μg/mL)...

Effect of dietary supplementation of ginger and turmeric rhizomes on ectonucleotidases, adenosine deaminase and acetylcholinesterase activities in synaptosomes from the cerebral cortex of hypertensive rats

Ayodele Jacob Akinyemi, Gustavo Roberto Thome, Vera Maria Morsch, Naiara Stefanello, Pauline da Costa, Andréia Cardoso, Jeferson Ferraz Goularte, Adriane Belló-Klein, Akintunde Afolabi Akindahunsi, Ganiyu Oboh, Maria Rosa Chitolina Schetinger

J Appl Biomed 14:59-70, 2016 | DOI: 10.1016/j.jab.2015.06.001  

Ginger and turmeric rhizomes are used in folk medicine for the treatment of several cerebrovascular diseases with limited scientific basis for their action. Hence, in this study, we investigate the effects of two Zingiberaceae varieties (ginger and turmeric) on ectonucleotidases (NTPDase and 5'-nucleotidase), adenosine deaminase (ADA) and acetylcholinesterase (AChE) activities in synaptosomes of cerebral cortex from l-NAME induced hypertensive rats. The animals were divided into seven groups (n = 10): normotensive control rats; hypertensive rats; hypertensive rats treated with atenolol; normotensive and hypertensive rats treated with 4% supplementation...

Enhanced hypoxic tolerance by Seabuckthorn is due to upregulation of HIF-1α and attenuation of ER stress

Kanika Jain, Geetha Suryakumar, Rajendra Prasad, Lilly Ganju, Shashi Bala Singh

J Appl Biomed 14:71-83, 2016 | DOI: 10.1016/j.jab.2015.10.001  

An imbalance in the redox homeostasis causes activation of multifaceted signaling responses which may be protective or deleterious. Amelioration of oxidative stress is one of the major modes of action of herbal supplements like Seabuckthorn (SBT). While the antioxidant potential of SBT is known, investigations into its effect on stress inducible signaling cascades are in progress. Here, we examine the impact of SBT on hypoxic tolerance and the mechanism behind its cardioprotective action. The efficacy of SBT was evaluated using the onset of gasping time (GT) at an altitude of 9754 m as the indicator for hypoxic tolerance. SBT led to a 100% increase...

The relationship between amniotic epithelial cells and their microenvironment

Katarzyna Kopaczka, Katarzyna Skowron, Emanuel Kolanko, Piotr Czekaj

J Appl Biomed 14:1-17, 2016 | DOI: 10.1016/j.jab.2015.10.004  

Human amniotic epithelial cells (hAEC) are characterized by a great ability to differentiate, and immunomodulatory properties toward mother's immune system cells. These features have been described as being able to change during pregnancy. Thanks to their unique properties: low immunogenicity and high effectiveness of transplantations, amniotic epithelial cells constitute a very attractive source of stem cells for practical purposes in regenerative medicine and transplantology. In this review, we focus on natural factors potentially determining hAEC immunophenotype during pregnancy. Recognition of the impact of specific factors on hAEC would help in...