Journal of Applied Biomedicine, 2017 (vol. 15), issue 2

Contents

J Appl Biomed 15:v, 2017 | DOI: 10.1016/S1214-021X(17)30130-8  

Lumbrokinase for degradation and reduction of amyloid fibrils associated with amyloidosis

Sanjay K. Metkar, Agnishwar Girigoswami, Ramachandran Murugesan, Koyeli Girigoswami

J Appl Biomed 15:96-104, 2017 | DOI: 10.1016/j.jab.2017.01.003  

Amyloidosis is a group of diseases caused by the accumulation of insoluble protein aggregates in different parts of the body. Repeated subcutaneous injection of insulin hormones in diabetic patients leads to localized amyloidosis that is found to be cytotoxic. Thus, agents that can dissociate these aggregates are critically needed. In the present study, insulin amyloid dissociation was demonstrated by the treatment of an enzyme lumbrokinase (LK) isolated from earthworm. Thioflavin T (ThT) fluorescence, solution turbidity, particle size analysis, FTIR, CD, atomic force microscopy and cell viability assay were employed to support the dissociation of...

One-pot and eco-friendly synthesis of silver nanocrystals using Adiantum raddianum: Toxicity against mosquito vectors of medical and veterinary importance

Marimuthu Govindarajan, Fatma Saeed AlQahtani, Mashael Marzouq AlShebly, Giovanni Benelli

J Appl Biomed 15:87-95, 2017 | DOI: 10.1016/j.jab.2016.10.004  

Mosquitoes (Diptera: Culicidae) represent a key threat for millions of humans and animals worldwide, since they act as vectors for devastating parasites and pathogens. Eco-friendly control tools are a priority. Plant-mediated biosynthesis of nanoparticles is rapid and cost-effective. Here we biosynthesized poly-dispersed silver nanocrystals (AgNPs) using a cheap aqueous leaf extract of Adiantum raddianum. AgNPs were characterized by UV-vis spectrophotometry, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX) and X-ray diffraction analysis...

Delta index of the estimated glomerular filtration rate to amend the overestimated Neutrophil Gelatinase-Associated Lipocalin (NGAL) level in systemic inflammatory response syndrome

Jong Weon Choi, Moon Hee Lee, Tatsuyoshi Fujii, Noriyoshi Fujii

J Appl Biomed 15:105-111, 2017 | DOI: 10.1016/j.jab.2016.12.002  

This study aimed to investigate a delta index of the estimated glomerular filtration rate (δeGFR-index) to amend an overestimated neutrophil gelatinase-associated lipocalin (NGAL) level in systemic inflammatory response syndrome (SIRS). A δeGFR-index was computed, and the NGAL/δeGFR-index was determined. Patients with SIRS exhibited significantly higher levels in NGAL, the δeGFR-index, and high sensitivity C-reactive protein (hsCRP) than non-SIRS inflammatory patients (216 ng/ml, 1.32, and 5.64 mg/dl vs. 153 ng/ml, 1.18, and 2.92 mg/dl, respectively, p < 0.05). After adjusting for potential confounders, the NGAL/δeGFR-index...

Electromagnetic field (10 Hz, 1 mT) protects mesenchymal stem cells from oxygen-glucose deprivation-induced cell death by reducing intracellular Ca²⁺ and reactive oxygen species

Jong Hyeok Jung, Jae Young Kim

J Appl Biomed 15:112-118, 2017 | DOI: 10.1016/j.jab.2016.11.003  

Protective effects of electromagnetic fields (EMFs) against oxygen and glucose deprivation (OGD)-induced human mesenchymal stem cell (MSC) death were studied. Cell survival, intracellular calcium and ROS/RNS levels were measured after culturing MSCs for 3 h under OGD with or without EMF exposure. The survival rate of cells cultured under OGD condition was significantly reduced compared to control cells, while cells cultured in OGD with 10 Hz/1 mT EMF exposure had higher survival ratio than that in equivalent non-exposed cells. This protective effect of EMF was not observed at different frequency/intensity combinations such as 10 Hz/0.01 mT, 10 Hz/0.1...

Assessment of antioxidant, antimicrobial and anti-osteosarcoma potential of four traditionally used Indian medicinal plants

Debasis Nayak, Sarbani Ashe, Pradipta Ranjan Rauta, Bismita Nayak

J Appl Biomed 15:119-132, 2017 | DOI: 10.1016/j.jab.2016.10.005  

In the present investigation Hibiscus rosa-sinensis (petals), Acorus calamus (rhizome), Moringa oleifera (leaves) and Cucurbita maxima (petals) were screened for their efficacy against osteosarcoma with elucidating a mechanism of their anticancer potentiality. The methanolic plant extracts revealed the presence of all major phytochemicals with quantitative analysis of flavonoids (98.15 ± 2.02 to 12.34 ± 0.57 mg of RUE/g) and total phenolics (26.40 ± 0.11 to 8.54 ± 0.10 mg of GAE/g). The antioxidant activity was assessed by standard DPPH, H₂O₂ scavenging, NO scavenging assays....

The effect of different doses of zinc supplementation on serum element and lactate levels in elite volleyball athletes

Gunay Eskici, Mehmet Gunay, Abdulkerim Kasim Baltaci, Rasim Mogulkoc

J Appl Biomed 15:133-138, 2017 | DOI: 10.1016/j.jab.2016.11.001  

The present study aims to examine the effect of different doses of zinc administration on serum element metabolism and fatigue. The study registered 20 female elite athletes. The subjects were divided into two groups in equal numbers. Group supplemented with 220 mg/day zinc sulfate. Group supplemented with 440 mg/day zinc sulfate. Athletes who were already engaged in their daily training routines were put to a 20-m shuttle run test to create fatigue before and after supplementation. Blood samples were collected from the subjects for a total of 7 times, before and after zinc supplementation and during rest and after exercise within one-week intervals...

Antibiotic treatment of asymptomatic Ureaplasma infection improves semen parameters in infertile men

Mohammad Hossein Ahmadi, Akbar Mirsalehian, Mohammad Ali Sadighi Gilani, Abbas Bahador, Malihe Talebi, Reza Salman Yazdi

J Appl Biomed 15:139-145, 2017 | DOI: 10.1016/j.jab.2016.11.004  

The role of asymptomatic infections caused by Ureaplasma species in male infertility and the efficacy of antibiotics in treatment of this failure is not yet definitely determined. A total of 165 infertile males having abnormal semen parameters (study group) as well as 165 healthy fertile men (control group) were included in this study. Semen samples were taken from all participants and after analyzing, undergone real-time PCR, microbial culture, and reactive oxygen species (ROS) as well as total antioxidant capacity (TAC) assays. Infected individuals of study group were treated with antibiotic. One month after the treatment completion, second...

Ioduria and type 1 diabetes mellitus - Relationships to selected clinical markers of diabetes in adults

Michala Vosátková, Denisa Žďárská Janíčková, Václav Zamrazil, Martina Salátová, Martin Hill, Karel Vondra

J Appl Biomed 15:146-150, 2017 | DOI: 10.1016/j.jab.2016.11.002  

There is a lack of data on the state of iodine reserves and the possible consequences of iodine deficits in diabetic patients. The main aims of this study were to: a) obtain information about iodine saturation in patients with type 1 diabetes; b) determine to what extent this saturation differs from the non-diabetic population; and c) determine whether iodine levels are related to several clinical and laboratory parameters characteristic of diabetic syndrome, including thyroid status.Subjects and methods: A total of 54 males and 51 females treated for type 1 diabetes mellitus (DM1) were included in this cross-sectional study. In addition to iodine...

Prediction of post-operative survival expectancy in thoracic lung cancer surgery with soft computing

Mohammad Saber Iraji

J Appl Biomed 15:151-159, 2017 | DOI: 10.1016/j.jab.2016.12.001  

Prediction of survival expectancy after surgery is so important. Soft computing approaches using training data are good approximations to model the different systems.We present many solutions to predict 1-year the post-operative survival expectancy in thoracic lung cancer surgery base on artificial intelligence. We implement multi-layer architecture of SUB- Adaptive neuro fuzzy inference system (MLA-ANFIS) approach with various combinations of multiple input features, neural networks, regression and ELM (extreme learning machine) based on the used thoracic surgery data set with sixteen input features. Our results contribute to the ELM (wave kernel)...