Journal of Applied Biomedicine, 2020 (vol. 18), issue 1
Original Research Article
Molecular design, synthesis and biological characterization of novel Resveratrol derivative as potential anticancer agent targeting NF-κB
Zuhier Awan, Hussam Ibrahim Kutbi, Aftab Ahmad, Rabbani Syed, Faten A. S. Alsulaimany, Noor Ahmad Shaik
J Appl Biomed 18:8-17, 2020 | DOI: 10.32725/jab.2020.001 
Resveratrol (RESV), an anticancer nutraceutical compound, is known to show poor bioavailability inside the human body. Therefore, this study has designed multiple chemical analogs of RESV compound for improving its pharmacokinetic as well as its anti-cancer properties. Initially, the drug likeliness and ADME-toxicity properties of these new chemical analogs were tested with the help of diverse computational approaches. Then the best predicted RESV derivative is synthesized by the organic method, and its NF-κB mediated anti-tumor activity assessed on histiocytic lymphoma U-937 cells. The new synthetic RESV analog, i.e. (E)-3-(prop-2-yn-1-yloxy)-5-(4-(prop-2-yn-1-yloxy)...
Toxicity assessment of Hypericum olympicum subsp. olympicum L. on human lymphocytes and breast cancer cell lines
Necmiye Balikci, Mehmet Sarimahmut, Ferda Ari, Nazlihan Aztopal, Mustafa Zafer Özel, Engin Ulukaya, Serap Celikler
J Appl Biomed 18:18-25, 2020 | DOI: 10.32725/jab.2020.002
There is a limited number of studies about the constituents of Hypericum olympicum subsp. olympicum and its genotoxic and cytotoxic potency. We examined the possible antigenotoxic/genotoxic properties of methanolic extract of H. olympicum subsp. olympicum (HOE) on human lymphocytes by employing sister chromatid exchange, micronucleus and comet assay and analyzed its chemical composition by GCxGC-TOF/MS. The anti-growth activity against MCF-7 and MDA-MB-231 cell lines was assessed by using the ATP viability assay. Cell death mode was investigated with fluorescence staining and ELISA assays. The major components of the flower...
A decision support system for the prediction of mortality in patients with acute kidney injury admitted in intensive care unit
Selda Kayaalti, Ömer Kayaalti, Bekir Hakan Aksebzeci
J Appl Biomed 18:26-32, 2020 | DOI: 10.32725/jab.2020.004
Intensive care unit (ICU) is a very special unit of a hospital, where healthcare professionals provide treatment and, later, close follow-up to the patients. It is crucial to estimate mortality in ICU patients from many viewpoints. The purpose of this study is to classify the status of patients with acute kidney injury (AKI) in ICU as early mortality, late mortality, and survival by the application of Classification and Regression Trees (CART) algorithm to the patients' attributes such as blood urea nitrogen, creatinine, serum and urine neutrophil gelatinase-associated lipocalin (NGAL), alkaline phosphatase, lactate dehydrogenase (LDH), gamma-glutamyl...
Review Article
The benefits of ascorbate to protect healthy cells in the prevention and treatment of oncological diseases
Pavel Klener, Matthew Scott Alexander, Joseph John Cullen, Věra Stejskal, Jiří Slíva, Lucie Kotlářová, Pavel Kostiuk, Zdeněk Procházka, Marta Kučerová
J Appl Biomed 18:1-7, 2020 | DOI: 10.32725/jab.2020.003
Health status is determined by the balance of oxidants and antioxidants which protects healthy cells against the threat of internal and external risk factors. Antioxidants such as ascorbate (vitamin C, ascorbic acid) are of fundamental importance in this respect. Ascorbate neutralizes potential damage caused by cellular oxidative stress which may be the greatest risk of damage to healthy tissue. Cellular oxidative stress is mediated by external factors (e.g. psychological stress, physical exertion, drugs, various diseases, environmental pollution, preservatives, smoking, and alcohol) and internal factors (products of cellular metabolism including reactive...